Strain identifier

BacDive ID: 11893

Type strain: Yes

Species: Mesorhizobium amorphae

Strain history: CIP <- 1999, E. Wang, Mexico Univ., Cuernavaca, Morelos, Mexico <- ACCC

NCBI tax ID(s): 71433 (species)

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General

@ref: 15978

BacDive-ID: 11893

DSM-Number: 21831

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium amorphae DSM 21831 is a mesophilic, Gram-negative, motile bacterium that was isolated from root nodule of Amorpha fruticosa.

NCBI tax id

  • NCBI tax id: 71433
  • Matching level: species

strain history

@refhistory
15978<- X. Sui, China Agric. Univ., College Biol. Sci., Beijing; ACCC 19665
67770IAM 15102 <-- LMG 18977 <-- P. de Lajudie R-2258 <-- E. Martínez-Romero <-- E. T. Wang.
116344CIP <- 1999, E. Wang, Mexico Univ., Cuernavaca, Morelos, Mexico <- ACCC

doi: 10.13145/bacdive11893.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium amorphae
  • full scientific name: Mesorhizobium amorphae Wang et al. 1999

@ref: 15978

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium amorphae

full scientific name: Mesorhizobium amorphae Wang et al. 1999

type strain: yes

Morphology

cell morphology

  • @ref: 116344
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 116344

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15978YMA-MEDIUM (MODIFIED) (DSMZ Medium 1031a)yeshttps://mediadive.dsmz.de/medium/1031aName: YMA-MEDIUM (modified) (DSMZ Medium 1031a) Composition: Agar 20.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l K2HPO4 0.5 g/l Na glutamate 0.5 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.0528 g/l NaCl 0.05 g/l FeCl3 x 6 H2O 0.00666 g/l Distilled water
38590MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
116344CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
15978positivegrowth28mesophilic
38590positivegrowth30mesophilic
67770positivegrowth28mesophilic
116344positivegrowth10-30
116344nogrowth37mesophilic
116344nogrowth41thermophilic
116344nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
116344NaClpositivegrowth0-2 %
116344NaClnogrowth4 %
116344NaClnogrowth6 %
116344NaClnogrowth8 %
116344NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116344esculin-hydrolysis4853
116344hippurate-hydrolysis606565
116344nitrate-reduction17632
116344nitrite-reduction16301
116344nitrate-respiration17632

metabolite production

  • @ref: 116344
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11634415688acetoin-
11634417234glucose-

enzymes

@refvalueactivityec
116344oxidase+
116344beta-galactosidase-3.2.1.23
116344alcohol dehydrogenase-1.1.1.1
116344gelatinase-
116344catalase+1.11.1.6
116344gamma-glutamyltransferase-2.3.2.2
116344lysine decarboxylase-4.1.1.18
116344ornithine decarboxylase-4.1.1.17
116344phenylalanine ammonia-lyase-4.3.1.24
116344tryptophan deaminase-
116344urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116344--++-++---++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116344---+/-+/-+/-+/----+/---------------------------+/-----+/-+/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116344++++++-+--+----+++++++-++-++++++++++++++--------+++--+--++++----------++-+-+--+++-+---+-++++-+++-+-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
15978root nodule of Amorpha fruticosaAmorpha fruticosaBeijingChinaCHNAsia
67770Nodule of Amorpha fruticosaAmorpha fruticosaBeijingChinaCHNAsia
116344Plant, Amorpha fruticosa, root noduleBeijingChinaCHNAsia1985

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_315.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_315&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: AF041442
  • Sequence Identity:
  • Total samples: 49047
  • soil counts: 28701
  • aquatic counts: 8865
  • animal counts: 7451
  • plant counts: 4030

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
159781Risk group (German classification)
1163441Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mesorhizobium amorphae gene for 16S rRNA, partial sequence, strain: NBRC 102496AB6818331410ena71433
20218Mesorhizobium amorphae strain LMG 18977 16S ribosomal RNA gene, partial sequence; internal transcribed spacer, complete sequence; and 23S ribosomal RNA, partial sequenceAF345257807ena71433
15978Mesorhizobium amorphae 16S ribosomal RNA gene, complete sequenceAF0414421473ena71433

External links

@ref: 15978

culture collection no.: DSM 21831, ACCC 19665, ATCC BAA 114, CIP 105910, JCM 21563, LMG 18977, NBRC 102496, IAM 15102, ICMP 15022, LMG 18261

straininfo link

  • @ref: 81110
  • straininfo: 12499

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028247Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov.Wang ET, van Berkum P, Sui XH, Beyene D, Chen WX, Martinez-Romero EInt J Syst Bacteriol10.1099/00207713-49-1-511999Base Composition, Base Sequence, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plasmids, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Rhizobiaceae/*classification/genetics, *Soil MicrobiologyGenetics
Phylogeny20087765Robinia pseudoacacia in Poland and Japan is nodulated by Mesorhizobium amorphae strains.Mierzwa B, Wdowiak-Wrobel S, Malek WAntonie Van Leeuwenhoek10.1007/s10482-010-9414-82010Alphaproteobacteria/genetics/*isolation & purification/*physiology, Bacterial Proteins/genetics, Base Composition, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, *Plant Root Nodulation, Poland, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Robinia/*microbiology/*physiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *SymbiosisGenetics
Phylogeny26296780Mesorhizobium waimense sp. nov. isolated from Sophora longicarinata root nodules and Mesorhizobium cantuariense sp. nov. isolated from Sophora microphylla root nodules.De Meyer SE, Wee Tan H, Heenan PB, Andrews M, Willems AInt J Syst Evol Microbiol10.1099/ijsem.0.0004302015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, New Zealand, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Sophora/*microbiologyGenetics
Phylogeny26704062Multilocus sequence analysis supports the taxonomic position of Astragalus glycyphyllos symbionts based on DNA-DNA hybridization.Gnat S, Malek W, Olenska E, Wdowiak-Wrobel S, Kalita M, Rogalski J, Wojcik MInt J Syst Evol Microbiol10.1099/ijsem.0.0008622015Astragalus Plant/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, Poland, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNATranscriptome
Phylogeny32193664Mesorhizobium alexandrii sp. nov., isolated from phycosphere microbiota of PSTs-producing marine dinoflagellate Alexandrium minutum amtk4.Yang X, Jiang Z, Zhang J, Zhou X, Zhang X, Wang L, Yu T, Wang Z, Bei J, Dong B, Dai Z, Yang Q, Chen ZAntonie Van Leeuwenhoek10.1007/s10482-020-01400-x2020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dinoflagellida/*microbiology, Fatty Acids/analysis, Genes, Bacterial, Mesorhizobium/*classification/genetics/*isolation & purification/physiology, *Microbiota, Nucleic Acid Hybridization, *Phylogeny, Quinones, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15978Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21831)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21831
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38590Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17982
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81110Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12499.1StrainInfo: A central database for resolving microbial strain identifiers
116344Curators of the CIPCollection of Institut Pasteur (CIP 105910)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105910