Strain identifier

BacDive ID: 11891

Type strain: Yes

Species: Mesorhizobium chacoense

Strain Designation: Pr5

Strain history: CIP <- 2001, E. Martinez Molina, Salamanca Univ., Salamanca, Spain: strain 19008

NCBI tax ID(s): 126843 (species)

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General

@ref: 6872

BacDive-ID: 11891

DSM-Number: 17287

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium chacoense Pr5 is a mesophilic, Gram-negative, motile bacterium that was isolated from root nodules of Prosopis alba.

NCBI tax id

  • NCBI tax id: 126843
  • Matching level: species

strain history

@refhistory
6872<- E. Velázquez, Univ. Salamanca, Spain; Pr5
118221CIP <- 2001, E. Martinez Molina, Salamanca Univ., Salamanca, Spain: strain 19008

doi: 10.13145/bacdive11891.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium chacoense
  • full scientific name: Mesorhizobium chacoense Velázquez et al. 2001

@ref: 6872

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium chacoense

full scientific name: Mesorhizobium chacoense Velázquez et al. 2001

strain designation: Pr5

type strain: yes

Morphology

cell morphology

  • @ref: 118221
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118221

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6872RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
33767MEDIUM 296 - for RhizobiumyesDistilled water make up to (800.000 ml);Agar (15.000 g);Yeast extract (1.000 g);Mannitol (10.000 g);Earth extract - M0541 (200.000 ml)
118221CIP Medium 296yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=296

culture temp

@refgrowthtypetemperaturerange
6872positivegrowth28mesophilic
33767positivegrowth30mesophilic
118221positivegrowth25-37mesophilic
118221nogrowth10psychrophilic
118221nogrowth41thermophilic
118221nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
118221NaClpositivegrowth0 %
118221NaClnogrowth2 %
118221NaClnogrowth4 %
118221NaClnogrowth6 %
118221NaClnogrowth8 %
118221NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate-assimilation24265
68369gelatin-hydrolysis5291
68369esculin+hydrolysis4853
68369urea+hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate-reduction17632
118221citrate-carbon source16947
118221esculin-hydrolysis4853
118221hippurate-hydrolysis606565
118221nitrate-reduction17632
118221nitrite-reduction16301

antibiotic resistance

  • @ref: 118221
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11822135581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11822115688acetoin-
11822117234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
118221oxidase+
118221beta-galactosidase-3.2.1.23
118221alcohol dehydrogenase-1.1.1.1
118221gelatinase-
118221amylase-
118221DNase-
118221caseinase-3.4.21.50
118221catalase-1.11.1.6
118221tween esterase-
118221gamma-glutamyltransferase+2.3.2.2
118221lecithinase-
118221lipase-
118221lysine decarboxylase-4.1.1.18
118221ornithine decarboxylase-4.1.1.17
118221phenylalanine ammonia-lyase-4.3.1.24
118221tryptophan deaminase-
118221urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118221-+++-+--+-++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6872----++-++/-+/-+/-+/-+/-+/----+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118221----------------------------+/---------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118221--------------------------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
6872root nodules of Prosopis albaProsopis albaChaco Arido regionArgentinaARGMiddle and South America
118221Plant, Root nodules of Prosopis albaChaco Arido regionArgentinaARGMiddle and South America1998

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root nodule

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68721Risk group (German classification)
1182211Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mesorhizobium chacoense strain LMG 19008 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceDQ659515738ena126843
6872Mesorhizobium chacoense 16S rRNA gene, strain PR5AJ2782491466ena126843

GC content

  • @ref: 6872
  • GC-content: 62.0
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 6872

culture collection no.: DSM 17287, CECT 5336, LMG 19008, CIP 107324

straininfo link

  • @ref: 81108
  • straininfo: 20638

literature

  • topic: Phylogeny
  • Pubmed-ID: 11411668
  • title: Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina).
  • authors: Velazquez E, Igual JM, Willems A, Fernandez MP, Munoz E, Mateos PF, Abril A, Toro N, Normand P, Cervantes E, Gillis M, Martinez-Molina E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-3-1011
  • year: 2001
  • mesh: Anti-Bacterial Agents/pharmacology, Argentina, Base Composition, Carbohydrate Metabolism, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Microbial Sensitivity Tests, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/microbiology, Plasmids, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Rhizobiaceae/*classification/drug effects/genetics/physiology, Rosales/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6872Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17287)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17287
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33767Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4747
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
81108Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID20638.1StrainInfo: A central database for resolving microbial strain identifiers
118221Curators of the CIPCollection of Institut Pasteur (CIP 107324)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107324