Strain identifier

BacDive ID: 1188

Type strain: No

Species: Bacillus spizizenii

Strain history: CIP <- 2001, C. Creff-Froger, AFSSA, Fougères, France: strain BGA, Bacillus subtilis

NCBI tax ID(s): 96241 (species)

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General

@ref: 323

BacDive-ID: 1188

DSM-Number: 618

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, motile, rod-shaped, human pathogen

description: Bacillus spizizenii DSM 618 is an obligate aerobe, mesophilic, Gram-positive human pathogen of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 96241
  • Matching level: species

strain history

@refhistory
323<- Inst. Veterinärmed. Bundesgesundheitsamt Berlin, BGA
123360CIP <- 2001, C. Creff-Froger, AFSSA, Fougères, France: strain BGA, Bacillus subtilis

doi: 10.13145/bacdive1188.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus spizizenii
  • full scientific name: Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus subtilis subsp. spizizenii

@ref: 323

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus spizizenii

full scientific name: Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

type strain: no

Morphology

cell morphology

  • @ref: 123360
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 123360

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
323NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35001MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123360CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
323positivegrowth30mesophilic
35001positivegrowth30mesophilic
123360positivegrowth22-45
123360nogrowth10psychrophilic
123360nogrowth55thermophilic

culture pH

  • @ref: 123360
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 123360
  • oxygen tolerance: obligate aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
323Mueller-Hinton Agar301aerob4034404434504436-3812-145038383030283622-243834102424814-163226403634-362040400222436
323Mueller-Hinton Agar30aerob40344044345042-44401050383630322634-36243634-361024-2624-2610-12163226403634-3612-144040020-2222-2436
323Mueller-Hinton Agar3728-302828-3030-32264842308463028222422281403082020612262430n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
323Mueller-Hinton Agar30aerob4436424840-42>50464018>5040403034283822-243838102628816362842383622-24424402424-2638
323Mueller-Hinton Agar30aerob4436424840-42>50464018>5040403034283822-243838102628816362842383622-24424402424-2638

halophily

  • @ref: 123360
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12336016947citrate-carbon source
1233604853esculin+hydrolysis
123360606565hippurate-hydrolysis
12336017632nitrate+reduction
12336016301nitrite-reduction
12336017632nitrate-respiration

metabolite production

  • @ref: 123360
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12336015688acetoin+
12336017234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123360oxidase-
123360beta-galactosidase+3.2.1.23
123360alcohol dehydrogenase-1.1.1.1
123360gelatinase+/-
123360amylase+
123360DNase+
123360caseinase+3.4.21.50
123360catalase+1.11.1.6
123360tween esterase-
123360gamma-glutamyltransferase+2.3.2.2
123360lecithinase-
123360lipase-
123360lysine decarboxylase-4.1.1.18
123360ornithine decarboxylase-4.1.1.17
123360urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123360--++------+----++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123360++-++-++-++----++++++++--------++++-+----+-++----+---+-+---++-+--+-------------++--------+++-+-----

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_21.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_20;99_21&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: DQ529249
  • Sequence Identity:
  • Total samples: 18201
  • soil counts: 3768
  • aquatic counts: 2854
  • animal counts: 9674
  • plant counts: 1905

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
323yes, in single cases1Risk group (German classification)
1233601Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Bacillus subtilis strain DSM 618 16S ribosomal RNA gene, partial sequence
  • accession: DQ529249
  • length: 1379
  • database: ena
  • NCBI tax ID: 1423

External links

@ref: 323

culture collection no.: DSM 618, CIP 107063

straininfo link

  • @ref: 70845
  • straininfo: 13196

literature

  • topic: Phylogeny
  • Pubmed-ID: 35902452
  • title: Antibiotic profiling of wild-type bacilli led to the discovery of new lanthipeptide subtilin-producing Bacillus spizizenii strains whose 16S rDNA sequences differ from the B. spizizenii typing strain.
  • authors: Helfrich M, Entian KD, Stein T
  • journal: Int Microbiol
  • DOI: 10.1007/s10123-022-00266-5
  • year: 2022
  • mesh: *Anti-Bacterial Agents/metabolism, *Bacillus/genetics, Bacillus subtilis/chemistry, Bacteriocins, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
323Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 618)https://www.dsmz.de/collection/catalogue/details/culture/DSM-618
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35001Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19262
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70845Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13196.1StrainInfo: A central database for resolving microbial strain identifiers
123360Curators of the CIPCollection of Institut Pasteur (CIP 107063)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107063