Strain identifier
BacDive ID: 11868
Type strain: 
Species: Hoeflea alexandrii
Strain Designation: AM1V30
Strain history: <- I. Marín, CSIC <- L. Palacios and I. Marín
NCBI tax ID(s): 288436 (species)
version 8.1 (current version)
General
@ref: 6553
BacDive-ID: 11868
DSM-Number: 16655
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Hoeflea alexandrii AM1V30 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from culture of the toxin-producing marine dinoflagellate Alexandrium minutum AL1V.
NCBI tax id
- NCBI tax id: 288436
- Matching level: species
strain history
- @ref: 6553
- history: <- I. Marín, CSIC <- L. Palacios and I. Marín
doi: 10.13145/bacdive11868.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Rhizobiaceae
- genus: Hoeflea
- species: Hoeflea alexandrii
- full scientific name: Hoeflea alexandrii Palacios et al. 2006
@ref: 6553
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Rhizobiaceae
genus: Hoeflea
species: Hoeflea alexandrii
full scientific name: Hoeflea alexandrii Palacios et al. 2006
strain designation: AM1V30
type strain: yes
Morphology
cell morphology
- @ref: 31770
- gram stain: negative
- cell length: 2.5 µm
- cell width: 0.8 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 31770
- production: yes
Culture and growth conditions
culture medium
- @ref: 6553
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6553 | positive | growth | 30 | mesophilic |
| 31770 | positive | growth | 10-42 | |
| 31770 | positive | optimum | 30-42 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31770 | positive | growth | 06-09 | alkaliphile |
| 31770 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 31770
- oxygen tolerance: aerobe
spore formation
- @ref: 31770
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 31770 | NaCl | positive | growth | 0-11.8 % |
| 31770 | NaCl | positive | optimum | 0-6.8 % |
observation
- @ref: 31770
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31770 | 22599 | arabinose | + | carbon source |
| 31770 | 4853 | esculin | + | hydrolysis |
enzymes
- @ref: 31770
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
- @ref: 6553
- sample type: culture of the toxin-producing marine dinoflagellate Alexandrium minutum AL1V
- host species: Alexandrium minutum
- geographic location: Vigo, Instituto Español de Oceanografia
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Laboratory | #Lab enrichment |
| #Environmental | #Aquatic | #Marine |
| #Host | #Protozoa | #Dinoflagellate |
taxonmaps
- @ref: 69479
- File name: preview.99_1597.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_488;97_557;98_640;99_1597&stattab=map
- Last taxonomy: Hoeflea
- 16S sequence: AJ786600
- Sequence Identity:
- Total samples: 7663
- soil counts: 1521
- aquatic counts: 2709
- animal counts: 840
- plant counts: 2593
Safety information
risk assessment
- @ref: 6553
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6553
- description: Hoeflea alexandrii partial 16S rRNA gene, strain AM1V30T
- accession: AJ786600
- length: 1449
- database: ena
- NCBI tax ID: 288436
Genome sequences
- @ref: 66792
- description: Hoeflea alexandrii DSM 16655
- accession: GCA_024105735
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 288436
GC content
- @ref: 6553
- GC-content: 59.7
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 54.233 | no |
| gram-positive | no | 98.138 | no |
| anaerobic | no | 97.162 | no |
| aerobic | yes | 91.999 | yes |
| halophile | no | 58.208 | no |
| spore-forming | no | 96.58 | yes |
| motile | yes | 90.281 | yes |
| glucose-ferment | no | 92.712 | no |
| thermophile | no | 97.537 | yes |
| glucose-util | yes | 91.176 | no |
External links
@ref: 6553
culture collection no.: DSM 16655, CECT 5682
straininfo link
- @ref: 81085
- straininfo: 194449
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16902042 | Hoeflea alexandrii sp. nov., isolated from the toxic dinoflagellate Alexandrium minutum AL1V. | Palacios L, Arahal DR, Reguera B, Marin I | Int J Syst Evol Microbiol | 10.1099/ijs.0.64238-0 | 2006 | Animals, Base Composition, DNA, Bacterial/chemistry, Dinoflagellida/*microbiology, Fatty Acids/analysis, Marine Biology, Molecular Sequence Data, Phylogeny, Proteobacteria/chemistry/*classification/isolation & purification/physiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Spain, Species Specificity | Genetics |
| Phylogeny | 23159752 | Hoeflea suaedae sp. nov., an endophytic bacterium isolated from the root of the halophyte Suaeda maritima. | Chung EJ, Park JA, Pramanik P, Bibi F, Jeon CO, Chung YR | Int J Syst Evol Microbiol | 10.1099/ijs.0.045484-0 | 2012 | Bacterial Typing Techniques, Base Composition, Chenopodiaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phyllobacteriaceae/*classification/genetics/isolation & purification, *Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/analysis | Genetics |
| Phylogeny | 25899504 | Hoeflea olei sp. nov., a diesel-oil-degrading, anoxygenic, phototrophic bacterium isolated from backwaters and emended description of the genus Hoeflea. | Rahul K, Azmatunnisa M, Sasikala C, Ramana CV | Int J Syst Evol Microbiol | 10.1099/ijs.0.000277 | 2015 | Bacterial Typing Techniques, Bacteriochlorophyll A/chemistry, Base Composition, Carotenoids/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gasoline/*microbiology, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, Phyllobacteriaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, Water Pollutants, Chemical/*chemistry | Genetics |
| Phylogeny | 29603043 | Hoeflea prorocentri sp. nov., isolated from a culture of the marine dinoflagellate Prorocentrum mexicanum PM01. | Yang Q, Jiang ZW, Huang CH, Zhang RN, Li LZ, Yang G, Feng LJ, Yang GF, Zhang H, Zhang XL, Mu J | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1074-0 | 2018 | Aquatic Organisms/*microbiology, DNA, Bacterial, Dinoflagellida/*microbiology, Gram-Negative Aerobic Bacteria/chemistry/*classification/genetics/isolation & purification, Metabolomics/methods, Molecular Typing, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics | Phenotype |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6553 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16655) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16655 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31770 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28043 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 81085 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID194449.1 | StrainInfo: A central database for resolving microbial strain identifiers |