Strain identifier
BacDive ID: 11865
Type strain:
Species: Aquamicrobium lusatiense
Strain Designation: S1
Strain history: CIP <- 2000, U. Lechner, Martin Luther Univ., Halle, Germany: strain S1
NCBI tax ID(s): 89772 (species)
General
@ref: 4200
BacDive-ID: 11865
DSM-Number: 11099
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative
description: Aquamicrobium lusatiense S1 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge.
NCBI tax id
- NCBI tax id: 89772
- Matching level: species
strain history
@ref | history |
---|---|
4200 | <- U. Lechner; S1 |
120678 | CIP <- 2000, U. Lechner, Martin Luther Univ., Halle, Germany: strain S1 |
doi: 10.13145/bacdive11865.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Phyllobacteriaceae
- genus: Aquamicrobium
- species: Aquamicrobium lusatiense
- full scientific name: Aquamicrobium lusatiense (Fritsche et al. 1999) Kämpfer et al. 2009
synonyms
@ref synonym 20215 Defluvibacter lusatiensis 20215 Defluvibacter lusatiae
@ref: 4200
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Phyllobacteriaceae
genus: Aquamicrobium
species: Aquamicrobium lusatiense
full scientific name: Aquamicrobium lusatiense (Fritsche et al. 1999) Kämpfer et al. 2009
strain designation: S1
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.941 | ||
120678 | negative | rod-shaped | yes |
pigmentation
- @ref: 120678
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4200 | MINERAL MEDIUM PH 7.25 (DSMZ Medium 465) | yes | https://mediadive.dsmz.de/medium/465 | Name: MINERAL MEDIUM PH 7.25 (DSMZ Medium 465; with strain-specific modifications) Composition: Na2HPO4 x 2 H2O 3.5 g/l KH2PO4 1.0 g/l (NH4)2SO4 0.5 g/l MgCl2 x 6 H2O 0.1 g/l None 0.05 g/l Ca(NO3)2 x 4 H2O 0.05 g/l Yeast extract 0.05 g/l Na2-EDTA 0.0005 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l FeSO4 x 7 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water |
4200 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
40260 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
120678 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4200 | positive | growth | 30 | mesophilic |
40260 | positive | growth | 30 | mesophilic |
120678 | positive | growth | 25-41 | |
120678 | no | growth | 5 | psychrophilic |
120678 | no | growth | 10 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120678
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.97 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120678 | NaCl | positive | growth | 0-4 % |
120678 | NaCl | no | growth | 6 % |
120678 | NaCl | no | growth | 8 % |
120678 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
120678 | 4853 | esculin | - | hydrolysis |
120678 | 17632 | nitrate | - | reduction |
120678 | 16301 | nitrite | - | reduction |
120678 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 120678
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
120678 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
120678 | oxidase | + | |
120678 | beta-galactosidase | - | 3.2.1.23 |
120678 | alcohol dehydrogenase | - | 1.1.1.1 |
120678 | gelatinase | - | |
120678 | amylase | - | |
120678 | DNase | - | |
120678 | caseinase | - | 3.4.21.50 |
120678 | catalase | + | 1.11.1.6 |
120678 | tween esterase | - | |
120678 | lecithinase | - | |
120678 | lipase | - | |
120678 | lysine decarboxylase | - | 4.1.1.18 |
120678 | ornithine decarboxylase | - | 4.1.1.17 |
120678 | protease | - | |
120678 | tryptophan deaminase | - | |
120678 | urease | - | 3.5.1.5 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
4200 | - | - | - | - | - | - | - | - | + | - | + | - | + | - | + | - | - | + | - | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120678 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
4200 | activated sludge | Schwarzheide | Germany | DEU | Europe | |
120678 | Environment, Activated sludge, industrial waste water treatment plant | Germany | DEU | Europe | 1988 |
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Activated sludge
taxonmaps
- @ref: 69479
- File name: preview.99_31812.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_3245;97_3983;98_23615;99_31812&stattab=map
- Last taxonomy: Aquamicrobium lusatiense subclade
- 16S sequence: AJ132378
- Sequence Identity:
- Total samples: 2524
- soil counts: 479
- aquatic counts: 765
- animal counts: 1097
- plant counts: 183
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4200 | 1 | Risk group (German classification) |
120678 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 4200
- description: Defluvibacter lusatiae 16S rRNA gene, partial, strain DSM11099T
- accession: AJ132378
- length: 1409
- database: ena
- NCBI tax ID: 89772
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Aquamicrobium lusatiense DSM 11099 | GCA_014201615 | scaffold | ncbi | 89772 |
66792 | Aquamicrobium lusatiense strain DSM 11099 | 89772.3 | wgs | patric | 89772 |
66792 | Aquamicrobium lusatiense DSM 11099 | 2861229971 | draft | img | 89772 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 80.687 | no |
gram-positive | no | 97.518 | no |
anaerobic | no | 97.877 | no |
aerobic | yes | 94.231 | no |
halophile | no | 85.726 | no |
spore-forming | no | 95.181 | no |
glucose-util | yes | 79.115 | no |
thermophile | no | 96.68 | yes |
motile | yes | 74.761 | no |
glucose-ferment | no | 91.753 | yes |
External links
@ref: 4200
culture collection no.: DSM 11099, CIP 106844
straininfo link
- @ref: 81082
- straininfo: 49030
literature
- topic: Phylogeny
- Pubmed-ID: 19622662
- title: Transfer of Defluvibacter lusatiensis to the genus Aquamicrobium as Aquamicrobium lusatiense comb. nov. and description of Aquamicrobium aerolatum sp. nov.
- authors: Kampfer P, Martin E, Lodders N, Jackel U
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.008730-0
- year: 2009
- mesh: Alphaproteobacteria/*classification/genetics/isolation & purification/metabolism, Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Ducks, Fatty Acids/chemistry/metabolism, Feces/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
4200 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11099) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11099 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
40260 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19019 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68369 | Automatically annotated from API 20NE | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
81082 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49030.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
120678 | Curators of the CIP | Collection of Institut Pasteur (CIP 106844) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106844 |