Strain identifier
BacDive ID: 11833
Type strain:
Species: Pelotomaculum thermopropionicum
Strain Designation: SI
Variant: Co-culture with BacDive ID 11834
Strain history: H. Imachi SI.
NCBI tax ID(s): 110500 (species)
General
@ref: 5142
BacDive-ID: 11833
DSM-Number: 13744
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Pelotomaculum thermopropionicum SI is an anaerobe, thermophilic bacterium that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor.
NCBI tax id
- NCBI tax id: 110500
- Matching level: species
strain history
@ref | history |
---|---|
5142 | <- H. Imachi, Nagaoka Univ. Technology, Japan; SI <- H. Imachi {1999} |
67770 | H. Imachi SI. |
doi: 10.13145/bacdive11833.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Peptococcaceae
- genus: Pelotomaculum
- species: Pelotomaculum thermopropionicum
- full scientific name: Pelotomaculum thermopropionicum Imachi et al. 2002
@ref: 5142
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Peptococcaceae
genus: Pelotomaculum
species: Pelotomaculum thermopropionicum
full scientific name: Pelotomaculum thermopropionicum Imachi et al. 2002
strain designation: SI
variant: Co-culture with BacDive ID 11834
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5142
- name: PELOTOMACULUM MEDIUM (DSMZ Medium 960)
- growth: yes
- link: https://mediadive.dsmz.de/medium/960
- composition: Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960) Composition: Na-pyruvate 2.19561 g/l Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Yeast extract 0.0998004 g/l Nitrilotriacetic acid 0.0127745 g/l NaCl 0.000998004 g/l FeCl2 x 4 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l ZnCl2 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5142 | positive | growth | 55 | thermophilic |
67770 | positive | growth | 55 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 5142
- oxygen tolerance: anaerobe
observation
- @ref: 67770
- observation: quinones: MK-7, MK-7(H4)
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
5142 | granular sludge in a thermophilic upflow anaerobic sludge blanket reactor | Japan | JPN | Asia |
67770 | Methanogenic granular sludge in a thermophilic upflow anaerobic sludge blanket reactor |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Bioreactor | |
#Engineered | #Waste | #Activated sludge |
#Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 5142
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5142
- description: Pelotomaculum thermopropionicum SI gene for 16S rRNA
- accession: AB035723
- length: 1532
- database: ena
- NCBI tax ID: 370438
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pelotomaculum thermopropionicum SI | GCA_000010565 | complete | ncbi | 370438 |
66792 | Pelotomaculum thermopropionicum SI | 370438.4 | complete | patric | 370438 |
66792 | Pelotomaculum thermopropionicum SI | 640427128 | complete | img | 370438 |
GC content
@ref | GC-content | method |
---|---|---|
5142 | 52.8 | |
67770 | 52.8 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 77.701 | no |
flagellated | no | 92.981 | no |
gram-positive | no | 90.824 | no |
anaerobic | yes | 99.709 | yes |
halophile | no | 96.291 | no |
spore-forming | yes | 90.049 | no |
thermophile | yes | 99.998 | yes |
glucose-util | yes | 76.89 | no |
aerobic | no | 98.122 | yes |
glucose-ferment | no | 79.182 | no |
External links
@ref: 5142
culture collection no.: DSM 13744, JCM 10971
straininfo link
- @ref: 81052
- straininfo: 100857
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12361280 | Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium. | Imachi H, Sekiguchi Y, Kamagata Y, Hanada S, Ohashi A, Harada H | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1729 | 2002 | Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Fermentation, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Peptococcaceae/*classification/genetics/isolation & purification/metabolism, Phenotype, Phylogeny, Propionates/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic | Stress |
Metabolism | 27342638 | In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria. | Tajima K, Han X, Hashimoto Y, Satoh Y, Satoh T, Taguchi S | J Biosci Bioeng | 10.1016/j.jbiosc.2016.06.001 | 2016 | 3-Hydroxybutyric Acid/metabolism, Acetate-CoA Ligase/isolation & purification/*metabolism, Acetyl Coenzyme A/metabolism, Acyltransferases/isolation & purification/*metabolism, Coenzyme A-Transferases/isolation & purification/*metabolism, Enzyme Stability, Hydroxybutyrates/metabolism, Lactic Acid/metabolism, Peptococcaceae/*enzymology/metabolism, Polyesters/metabolism, Polyhydroxyalkanoates/*biosynthesis, Temperature, Thermus thermophilus/*enzymology/metabolism | Enzymology |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
5142 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13744) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13744 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
81052 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100857.1 | StrainInfo: A central database for resolving microbial strain identifiers |