Strain identifier

BacDive ID: 11833

Type strain: Yes

Species: Pelotomaculum thermopropionicum

Strain Designation: SI

Variant: Co-culture with BacDive ID 11834

Strain history: H. Imachi SI.

NCBI tax ID(s): 110500 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5142

BacDive-ID: 11833

DSM-Number: 13744

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic

description: Pelotomaculum thermopropionicum SI is an anaerobe, thermophilic bacterium that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor.

NCBI tax id

  • NCBI tax id: 110500
  • Matching level: species

strain history

@refhistory
5142<- H. Imachi, Nagaoka Univ. Technology, Japan; SI <- H. Imachi {1999}
67770H. Imachi SI.

doi: 10.13145/bacdive11833.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptococcaceae
  • genus: Pelotomaculum
  • species: Pelotomaculum thermopropionicum
  • full scientific name: Pelotomaculum thermopropionicum Imachi et al. 2002

@ref: 5142

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptococcaceae

genus: Pelotomaculum

species: Pelotomaculum thermopropionicum

full scientific name: Pelotomaculum thermopropionicum Imachi et al. 2002

strain designation: SI

variant: Co-culture with BacDive ID 11834

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 5142
  • name: PELOTOMACULUM MEDIUM (DSMZ Medium 960)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/960
  • composition: Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960) Composition: Na-pyruvate 2.19561 g/l Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Yeast extract 0.0998004 g/l Nitrilotriacetic acid 0.0127745 g/l NaCl 0.000998004 g/l FeCl2 x 4 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l ZnCl2 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5142positivegrowth55thermophilic
67770positivegrowth55thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5142
  • oxygen tolerance: anaerobe

observation

  • @ref: 67770
  • observation: quinones: MK-7, MK-7(H4)

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5142granular sludge in a thermophilic upflow anaerobic sludge blanket reactorJapanJPNAsia
67770Methanogenic granular sludge in a thermophilic upflow anaerobic sludge blanket reactor

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Waste#Activated sludge
#Condition#Anoxic (anaerobic)

Safety information

risk assessment

  • @ref: 5142
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5142
  • description: Pelotomaculum thermopropionicum SI gene for 16S rRNA
  • accession: AB035723
  • length: 1532
  • database: ena
  • NCBI tax ID: 370438

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pelotomaculum thermopropionicum SIGCA_000010565completencbi370438
66792Pelotomaculum thermopropionicum SI370438.4completepatric370438
66792Pelotomaculum thermopropionicum SI640427128completeimg370438

GC content

@refGC-contentmethod
514252.8
6777052.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes77.701no
flagellatedno92.981no
gram-positiveno90.824no
anaerobicyes99.709yes
halophileno96.291no
spore-formingyes90.049no
thermophileyes99.998yes
glucose-utilyes76.89no
aerobicno98.122yes
glucose-fermentno79.182no

External links

@ref: 5142

culture collection no.: DSM 13744, JCM 10971

straininfo link

  • @ref: 81052
  • straininfo: 100857

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12361280Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium.Imachi H, Sekiguchi Y, Kamagata Y, Hanada S, Ohashi A, Harada HInt J Syst Evol Microbiol10.1099/00207713-52-5-17292002Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Fermentation, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Peptococcaceae/*classification/genetics/isolation & purification/metabolism, Phenotype, Phylogeny, Propionates/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicStress
Metabolism27342638In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria.Tajima K, Han X, Hashimoto Y, Satoh Y, Satoh T, Taguchi SJ Biosci Bioeng10.1016/j.jbiosc.2016.06.00120163-Hydroxybutyric Acid/metabolism, Acetate-CoA Ligase/isolation & purification/*metabolism, Acetyl Coenzyme A/metabolism, Acyltransferases/isolation & purification/*metabolism, Coenzyme A-Transferases/isolation & purification/*metabolism, Enzyme Stability, Hydroxybutyrates/metabolism, Lactic Acid/metabolism, Peptococcaceae/*enzymology/metabolism, Polyesters/metabolism, Polyhydroxyalkanoates/*biosynthesis, Temperature, Thermus thermophilus/*enzymology/metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitle
5142Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13744)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13744
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
81052Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100857.1StrainInfo: A central database for resolving microbial strain identifiers