Strain identifier

BacDive ID: 11766

Type strain: No

Species: Desulfitobacterium hafniense

Strain history: <- ATCC <- R. Beaudet, PCP-1

NCBI tax ID(s): 49338 (species)

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General

@ref: 4551

BacDive-ID: 11766

DSM-Number: 12420

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Desulfitobacterium hafniense DSM 12420 is an anaerobe, mesophilic bacterium that was isolated from methanogenic consortium from mixture of anaerobic sewage sludge and soil.

NCBI tax id

  • NCBI tax id: 49338
  • Matching level: species

strain history

  • @ref: 4551
  • history: <- ATCC <- R. Beaudet, PCP-1

doi: 10.13145/bacdive11766.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Desulfitobacteriaceae
  • genus: Desulfitobacterium
  • species: Desulfitobacterium hafniense
  • full scientific name: Desulfitobacterium hafniense Christiansen and Ahring 1996

  • synonyms

    • @ref: 20215
    • synonym: Desulfitobacterium frappieri

@ref: 4551

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptococcaceae

genus: Desulfitobacterium

species: Desulfitobacterium hafniense

full scientific name: Desulfitobacterium hafniense Christiansen and Ahring 1996 emend. Niggemyer et al. 2001

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4551DESULFITOBACTERIUM HAFNIENSE MEDIUM (DSMZ Medium 720)yeshttps://mediadive.dsmz.de/medium/720Name: DESULFITOBACTERIUM HAFNIENSE MEDIUM (DSMZ Medium 720) Composition: NaHCO3 2.59222 g/l Na-pyruvate 2.49252 g/l Na2S2O3 x 5 H2O 1.24626 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l K2HPO4 x 3 H2O 0.398804 g/l Na2S x 9 H2O 0.299103 g/l MgCl2 x 6 H2O 0.0997009 g/l NaCl 0.0997009 g/l CaCl2 x 2 H2O 0.0498504 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Thiamine HCl 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
4551DESULFITOBACTERIUM FRAPPIERI MEDIUM (DSMZ Medium 836)yeshttps://mediadive.dsmz.de/medium/836Name: DESULFITOBACTERIUM PCP-1 MEDIUM (DSMZ Medium 836) Composition: Na-pyruvate 6.05 g/l Yeast extract 1.0 g/l Na2SO3 0.63 g/l NH4Cl 0.53 g/l Na2CO3 0.5 g/l K2HPO4 0.35 g/l KH2PO4 0.27 g/l MgCl2 x 6 H2O 0.1 g/l CaCl2 x 2 H2O 0.07 g/l Na2S x 9 H2O 0.05 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

  • @ref: 4551
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4551
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4551
  • sample type: methanogenic consortium from mixture of anaerobic sewage sludge and soil
  • geographic location: Quebec
  • country: Canada
  • origin.country: CAN
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Sewage sludge
#Environmental#Microbial community
#Environmental#Terrestrial#Soil
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_832.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_99;96_501;97_574;98_660;99_832&stattab=map
  • Last taxonomy: Desulfitobacterium hafniense subclade
  • 16S sequence: U40078
  • Sequence Identity:
  • Total samples: 874
  • soil counts: 503
  • aquatic counts: 132
  • animal counts: 217
  • plant counts: 22

Safety information

risk assessment

  • @ref: 4551
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4551
  • description: Desulfitobacterium frappieri 16S ribosomal RNA gene, complete sequence
  • accession: U40078
  • length: 1655
  • database: ena
  • NCBI tax ID: 49338

GC content

  • @ref: 4551
  • GC-content: 46
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4551

culture collection no.: DSM 12420, ATCC 700357

straininfo link

  • @ref: 80984
  • straininfo: 43890

Reference

@idauthorscataloguedoi/urltitle
4551Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12420)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12420
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80984Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43890.1StrainInfo: A central database for resolving microbial strain identifiers