We are hiring!

Strain identifier

BacDive ID: 11754

Type strain: No

Species: Aggregatibacter actinomycetemcomitans

Strain Designation: Y4

Strain history: Tokyo Med. Dent. Univ. <-- ATCC 43718 <-- H. Reynolds Y4 <-- S. S. Socransky.

NCBI tax ID(s): 1035194 (strain), 714 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7.1 (current version):
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7.1 (current version)

General

@ref: 4215

BacDive-ID: 11754

DSM-Number: 11123

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, human pathogen

description: Aggregatibacter actinomycetemcomitans Y4 is a microaerophile, mesophilic human pathogen that was isolated from subgingival dental plaque.

NCBI tax id

NCBI tax idMatching level
1035194strain
714species

strain history

doi: 10.13145/bacdive11754.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Aggregatibacter
  • species: Aggregatibacter actinomycetemcomitans
  • full scientific name: Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006
  • synonyms

    @refsynonym
    20215Actinobacillus actinomycetemcomitans
    20215Haemophilus actinomycetemcomitans
    20215Bacterium actinomycetemcomitans

@ref: 4215

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Aggregatibacter

species: Aggregatibacter actinomycetemcomitans

full scientific name: Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006

strain designation: Y4

type strain: no

Morphology

colony morphology

  • @ref: 4215
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4215BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
4215COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
4215positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4215
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837727897tryptophan-energy source
6837716199urea-hydrolysis
6837718257ornithine-degradation
6837717634D-glucose+builds acid from

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4215catalase+1.11.1.6
4215cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
4215-+++----+--+-
4215++-----+--+--
4215++-----+--+--
4215+++----+--+--
4215++++---+--+--
4215-++++/----+--+-
4215-++-----+--+-
4215-++-----+--+-
4215-++++---+--+-
4215-+++----+--+-

Isolation, sampling and environmental information

isolation

  • @ref: 4215
  • sample type: subgingival dental plaque

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways#Subgingival plaque
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_570.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_321;97_406;98_465;99_570&stattab=map
  • Last taxonomy: Aggregatibacter actinomycetemcomitans subclade
  • 16S sequence: AB595137
  • Sequence Identity:
  • Total samples: 8336
  • soil counts: 46
  • aquatic counts: 279
  • animal counts: 7969
  • plant counts: 42

Safety information

risk assessment

  • @ref: 4215
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus actinomycetemcomitans strain ATCC 43718 16S ribosomal RNA gene, partial sequenceAY3628841362ena1035194
67770Aggregatibacter actinomycetemcomitans gene for 16S ribosomal RNA, partial sequence, strain: JCM 8578AB5951371501ena714

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aggregatibacter actinomycetemcomitans Y4 Y4GCA_000318155scaffoldncbi1035194
66792Aggregatibacter actinomycetemcomitans Y41035194.3wgspatric1035194
66792Aggregatibacter actinomycetemcomitans Y42537561947draftimg1035194
66792Echinicola sp. 20G 20GGCA_015533855completepatric2781961

External links

@ref: 4215

culture collection no.: DSM 11123, ATCC 43718, JCM 8578

straininfo link

@refpassport
20218http://www.straininfo.net/strains/162502
20218http://www.straininfo.net/strains/113048

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1381732Identification and analysis of the gap region in the 23S ribosomal RNA from Actinobacillus actinomycetemcomitans.Haraszthy VI, Sunday GJ, Bobek LA, Motley TS, Preus H, Zambon JJJ Dent Res10.1177/002203459207100904011992Aggregatibacter actinomycetemcomitans/*genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics, DNA, Ribosomal/*genetics, DNA, Single-Stranded/genetics, Electrophoresis, Agar Gel, *Genes, Bacterial, Molecular Sequence Data, Polymerase Chain Reaction, *RNA Processing, Post-Transcriptional, RNA, Bacterial/genetics, RNA, Ribosomal, 23S/*genetics, Restriction Mapping, Single-Strand Specific DNA and RNA EndonucleasesGenetics
Pathogenicity1706323Evidence that the serotype b antigenic determinant of Actinobacillus actinomycetemcomitans Y4 resides in the polysaccharide moiety of lipopolysaccharide.Wilson ME, Schifferle REInfect Immun10.1128/iai.59.4.1544-1551.19911991Actinobacillus/*immunology, Adolescent, Adult, Age Factors, Antibodies, Bacterial/analysis, Antigens, Bacterial/analysis/*immunology, Child, Epitopes/*analysis, Humans, Immunodiffusion, Immunoglobulin G/analysis, Lipopolysaccharides/analysis/*immunology, Periodontitis/immunology/microbiology, Polysaccharides, Bacterial/analysis/*immunology
Metabolism7927715Identification of an immunoglobulin Fc receptor of Actinobacillus actinomycetemcomitans.Mintz KP, Fives-Taylor PMInfect Immun10.1128/iai.62.10.4500-4505.19941994Aggregatibacter actinomycetemcomitans/*immunology, Amino Acid Sequence, Avidin/metabolism, Bacterial Outer Membrane Proteins/*analysis, Humans, Immunoglobulin Fc Fragments/metabolism, Molecular Sequence Data, Receptors, Fc/*analysis/chemistry/physiologyPathogenicity
Enzymology8837475Isolation and characterization of hemolytic genes from Actinobacillus actinomycetemcomitans.Kato T, Kimizuka R, Okuda KFEMS Microbiol Lett10.1111/j.1574-6968.1996.tb08483.x1996Aggregatibacter actinomycetemcomitans/*genetics/*pathogenicity, Animals, Cloning, Molecular, DNA, Bacterial/genetics, Escherichia coli/genetics, *Genes, Bacterial, Hemolysin Proteins/genetics, Hemolysis/*genetics, Horses, Humans, In Vitro Techniques, Rabbits, Restriction Mapping, SheepPathogenicity
Enzymology9916112Cloning of the gene encoding the Actinobacillus actinomycetemcomitans serotype b OmpA-like outer membrane protein.Komatsuzawa H, Kawai T, Wilson ME, Taubman MA, Sugai M, Suginaka HInfect Immun10.1128/IAI.67.2.942-945.19991999Aggregatibacter actinomycetemcomitans/*genetics, Amino Acid Sequence, Animals, Bacterial Outer Membrane Proteins/*genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial, Escherichia coli, Gene Expression, Genes, Bacterial, Mice, Mice, Inbred BALB C, Molecular Sequence DataGenetics
Pathogenicity10093529Antigenic components of Actinobacillus actinomycetemcomitans lipopolysaccharide recognized by sera from patients with localized juvenile periodontitis.Gu K, Bainbridge B, Darveau RP, Page RCOral Microbiol Immunol10.1111/j.1399-302x.1998.tb00726.x1998Adolescent, Aggregatibacter actinomycetemcomitans/*immunology, Aggressive Periodontitis/blood/*immunology, Antibodies, Bacterial/biosynthesis/blood, Antibody Formation, Antigens, Bacterial/chemistry, Child, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G/biosynthesis/blood, Lipid A/immunology, Lipopolysaccharides/*chemistry/*immunology, O Antigens/immunology, Oligosaccharides/immunology
Phylogeny10588502Bacterial adhesion of Actinobacillus actinomycetemcomitans serotypes to titanium implants: SEM evaluation. A preliminary report.Okte E, Sultan N, Dogan B, Asikainen SJ Periodontol10.1902/jop.1999.70.11.13761999Aggregatibacter actinomycetemcomitans/classification/*physiology, *Bacterial Adhesion, Blade Implantation, Dental Implants/*microbiology, Microscopy, Electron, Scanning, Species Specificity
Phylogeny10767609Heterogeneity in the immune response to serotype b LPS of Actinobacillus actinomycetemcomitans in inbred strains of mice.Kato T, Honma K, Yamanaka A, Miura T, Okuda KFEMS Immunol Med Microbiol10.1111/j.1574-695X.2000.tb01458.x2000Actinobacillus Infections/immunology/microbiology, Aggregatibacter actinomycetemcomitans/classification/*immunology, Animals, Antibodies, Bacterial/*blood, Cytokines/metabolism, Enzyme-Linked Immunosorbent Assay, Female, Immunization, Lipopolysaccharides/*immunology, Mice, Mice, Inbred Strains, SerotypingMetabolism
Pathogenicity10899863Novel apoptosis-inducing activity in Bacteroides forsythus: a comparative study with three serotypes of Actinobacillus actinomycetemcomitans.Arakawa S, Nakajima T, Ishikura H, Ichinose S, Ishikawa I, Tsuchida NInfect Immun10.1128/IAI.68.8.4611-4615.20002000Aggregatibacter actinomycetemcomitans/classification/*pathogenicity, *Apoptosis, Bacterial Proteins/*toxicity, Bacteroides/*pathogenicity, Caspase 3, Caspases/metabolism, DNA Fragmentation, Enzyme Activation, HL-60 Cells, Humans, Jurkat Cells, Leukocytes/pathology, SerotypingPhylogeny
Pathogenicity10951380Influence of surface modifications to titanium on oral bacterial adhesion in vitro.Yoshinari M, Oda Y, Kato T, Okuda K, Hirayama AJ Biomed Mater Res10.1002/1097-4636(200011)52:2<388::aid-jbm20>3.0.co;2-e2000Aggregatibacter actinomycetemcomitans, *Bacterial Adhesion, *Dental Materials, Humans, Oral Health, Porphyromonas gingivalis, Surface Properties, *Titanium
Pathogenicity11426884Influence of surface modifications to titanium on antibacterial activity in vitro.Yoshinari M, Oda Y, Kato T, Okuda KBiomaterials10.1016/s0142-9612(00)00392-62001Aggregatibacter actinomycetemcomitans/*drug effects/growth & development/physiology, Animals, Bacterial Adhesion/*drug effects, Biocompatible Materials/*chemistry/pharmacology/toxicity, Calcium/pharmacology, Coated Materials, Biocompatible/chemistry/pharmacology/toxicity, Fluorine/pharmacology, L Cells/drug effects, Materials Testing, Mice, Mouth/microbiology, Nitrogen/pharmacology, Oxidation-Reduction, Porphyromonas gingivalis/*drug effects/growth & development/physiology, Surface Properties, Titanium/analysis/*chemistry/pharmacology/toxicity
Pathogenicity12019120Effect of ciprofloxacin on killing of Actinobacillus actinomycetemcomitans by polymorphonuclear leukocytes.Cacchillo DA, Walters JDAntimicrob Agents Chemother10.1128/AAC.46.6.1980-1984.20022002Aggregatibacter actinomycetemcomitans/*drug effects/*immunology, Anti-Infective Agents/*pharmacology, Ciprofloxacin/*pharmacology, Colony Count, Microbial, Humans, Immunity, Cellular/*drug effects, In Vitro Techniques, Neutrophils/*drug effects/*immunology, Oxidation-Reduction, Phagocytosis/*drug effects, Respiratory Burst/drug effects
16904783A simple viability-maintaining method produces homogenic cell suspensions of autoaggregating wild-type Actinobacillus actinomycetemcomitans.Karched M, Paul-Satyaseela M, Asikainen SJ Microbiol Methods10.1016/j.mimet.2006.06.0042006Aggregatibacter actinomycetemcomitans/growth & development/*physiology/ultrastructure, Colony Count, Microbial, Microscopy, Electron, Statistics, Nonparametric
Pathogenicity18173797Reduction of periodontal pathogens adhesion by antagonistic strains.Van Hoogmoed CG, Geertsema-Doornbusch GI, Teughels W, Quirynen M, Busscher HJ, Van der Mei HCOral Microbiol Immunol10.1111/j.1399-302X.2007.00388.x2008Actinomyces/physiology, Aggregatibacter actinomycetemcomitans/*physiology, Antibiosis/*physiology, Bacterial Adhesion/*physiology, Bacteriological Techniques, Colony Count, Microbial, Haemophilus parainfluenzae/physiology, Humans, Periodontitis/microbiology, Porphyromonas gingivalis/*physiology, Prevotella intermedia/*physiology, Streptococcus/classification/physiology, Streptococcus mitis/physiology, Streptococcus sanguis/physiologyPhylogeny
Metabolism18954350Differential effects of five Aggregatibacter actinomycetemcomitans strains on gingival epithelial cells.Shimada T, Sugano N, Nishihara R, Suzuki K, Tanaka H, Ito KOral Microbiol Immunol10.1111/j.1399-302X.2008.00449.x2008Actinobacillus Infections/*microbiology, Aggregatibacter actinomycetemcomitans/*pathogenicity, Cell Proliferation, Cells, Cultured, Epithelial Cells/metabolism/*microbiology, Gingiva/metabolism/*microbiology, Humans, Intercellular Adhesion Molecule-1/metabolism, Interleukin-8/metabolism, RNA, Messenger/metabolism, Species SpecificityPathogenicity
Metabolism20716951Differential epithelial cell response upon stimulation with the Aggregatibacter actinomycetemcomitans strains VT 1169, VT 1560 DAM(-) and ATCC 4318.Eberhard J, Banasch T, Jepsen S, Dommisch HEpigenetics10.4161/epi.5.8.131062010Adult, Bacterial Proteins/genetics/immunology/*metabolism, Epithelial Cells/immunology/*metabolism/microbiology, Female, *Gene Expression Regulation, Humans, Male, Mouth Diseases/genetics/immunology/*metabolism/microbiology, Pasteurellaceae/genetics/immunology/*metabolism, RNA, Messenger/biosynthesis/immunology, Site-Specific DNA-Methyltransferase (Adenine-Specific)Pathogenicity
Pathogenicity21420071Influence of different biomaterials on the viability of Aggregatibacter actinomycetemcomitans.Kasaj A, Willershausen B, Junker R, Callaway A, Krahn U, Kraft B, Pietsch MArch Oral Biol10.1016/j.archoralbio.2011.02.0132011Aggregatibacter actinomycetemcomitans/*drug effects, Animals, Biocompatible Materials/*pharmacology, Bone Matrix/physiology, Calcium Hydroxide/pharmacology, Calcium Phosphates/pharmacology, Cattle, Dental Enamel Proteins/pharmacology, Durapatite/pharmacology, Least-Squares Analysis, Linear Models, Materials Testing, Microbial Viability/drug effects, Nanostructures, Regenerative Medicine/methods
Pathogenicity25327222Antimicrobial effect of lemongrass oil against oral malodour micro-organisms and the pilot study of safety and efficacy of lemongrass mouthrinse on oral malodour.Satthanakul P, Taweechaisupapong S, Paphangkorakit J, Pesee M, Timabut P, Khunkitti WJ Appl Microbiol10.1111/jam.126672014Adult, Aggregatibacter actinomycetemcomitans/drug effects, Anti-Bacterial Agents/adverse effects/pharmacology/*therapeutic use, Double-Blind Method, Female, Halitosis/*drug therapy/microbiology, Humans, Male, Mouthwashes/adverse effects/pharmacology/*therapeutic use, Pilot Projects, Plant Oils/adverse effects/chemistry/pharmacology/*therapeutic use, Porphyromonas gingivalis/drug effects, Streptococcus mutans/drug effects, Terpenes/adverse effects/chemistry/pharmacology/*therapeutic use, Young Adult
Pathogenicity28823045Killing activity of LFchimera on periodontopathic bacteria and multispecies oral biofilm formation in vitro.Ruangcharoen S, Suwannarong W, Lachica MRCT, Bolscher JGM, Nazmi K, Khunkitti W, Taweechaisupapong SWorld J Microbiol Biotechnol10.1007/s11274-017-2334-22017Aggregatibacter actinomycetemcomitans/drug effects/physiology, Anti-Bacterial Agents/*pharmacology, Antimicrobial Cationic Peptides/*pharmacology, Biofilms/*drug effects, Humans, In Vitro Techniques, Microbial Sensitivity Tests, Microbial Viability/drug effects, Periodontitis/*microbiology, Plankton/drug effects
Pathogenicity31329599Synergistic effects of LFchimera and antibiotic against planktonic and biofilm form of Aggregatibacter actinomycetemcomitans.Lachica MRCT, Anutrakunchai C, Prajaneh S, Nazmi K, Bolscher JGM, Taweechaisupapong SPLoS One10.1371/journal.pone.02172052019Aggregatibacter actinomycetemcomitans/*physiology, Anti-Bacterial Agents/chemistry/*pharmacology, Antimicrobial Cationic Peptides/agonists/chemistry/genetics/*pharmacology, Biofilms/*drug effects/growth & development, Drug Synergism, Humans, Lactoferrin/agonists/chemistry/genetics/*pharmacology, Periodontitis/drug therapy/microbiology, Plankton/*growth & development, Recombinant Fusion Proteins/chemistry/genetics/pharmacology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4215Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11123)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11123
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta