Strain identifier

BacDive ID: 11741

Type strain: Yes

Species: Pasteurella testudinis

Strain Designation: 90-23-79n

Strain history: CIP <- 1982, ATCC <- K.P. Snipes, California Univ., USA: strain 90-23-79n

NCBI tax ID(s): 1122938 (strain), 761 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16690

BacDive-ID: 11741

DSM-Number: 23072

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, animal pathogen

description: Pasteurella testudinis 90-23-79n is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from desert tortoise nares Gopherus agassizi.

NCBI tax id

NCBI tax idMatching level
1122938strain
761species

strain history

@refhistory
16690<- CCUG <- R. Mutters, Marburg, Germany <- ATCC <- K. P. Snipes, UCD
123486CIP <- 1982, ATCC <- K.P. Snipes, California Univ., USA: strain 90-23-79n

doi: 10.13145/bacdive11741.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Pasteurella
  • species: Pasteurella testudinis
  • full scientific name: Pasteurella testudinis Snipes and Biberstein 1982

@ref: 16690

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Pasteurella

species: Pasteurella testudinis

full scientific name: Pasteurella testudinis Snipes and Biberstein 1982

strain designation: 90-23-79n

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.987

colony morphology

  • @ref: 16690
  • type of hemolysis: beta
  • hemolysis ability: 1

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_23072_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23072_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23072_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23072_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23072_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16690COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16690BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38681MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicolayesDistilled water make up to (1000.000 ml);Yeast extract (1.000 g);Tryptocasein soy agar (40.000 g)
123486CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b

culture temp

@refgrowthtypetemperaturerange
16690positivegrowth37mesophilic
38681positivegrowth37mesophilic
47060positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 47060
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6837717634D-glucose+builds acid from
6837715824D-fructose+builds acid from
6837717306maltose+builds acid from
6837718257ornithine-degradation

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
16690catalase+1.11.1.6
16690cytochrome-c oxidase+1.9.3.1
68377proline-arylamidase-3.4.11.5
68377ornithine decarboxylase-4.1.1.17
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
16690++---+-+---+/-+/-+/-+/---+--+

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16690++++--+-+--+-
16690-++++--+-+--+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
16690desert tortoise nares Gopherus agassiziGopherus agassizisouthern California, Mojave DesertUSAUSANorth America
47060Desert tortoise nares;Gopherus agassiziCalifornia,Mojare desertUSAUSANorth America
123486Desert tortoise, naresCaliforniaUnited States of AmericaUSANorth America1981

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Desert
#Host#Reptilia

taxonmaps

  • @ref: 69479
  • File name: preview.99_5931.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_2833;97_3484;98_4407;99_5931&stattab=map
  • Last taxonomy: Pasteurella testudinis subclade
  • 16S sequence: AY362926
  • Sequence Identity:
  • Total samples: 635
  • soil counts: 10
  • aquatic counts: 79
  • animal counts: 544
  • plant counts: 2

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16690yes2Risk group (German classification)
1234862Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pasteurella testudinis DSM 23072 strain CCUG 19802 16S ribosomal RNA gene, partial sequenceL060901495ena1122938
16690Pasteurella testudinis DSM 23072 strain CCUG 19802 16S ribosomal RNA gene, partial sequenceAY3629261371ena1122938

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pasteurella testudinis NCTC12150GCA_900454705contigncbi761
66792Pasteurella testudinis DSM 23072GCA_019633665scaffoldncbi1122938
66792Pasteurella testudinis DSM 23072GCA_900176075scaffoldncbi1122938
66792Pasteurella testudinis DSM 230721122938.4wgspatric1122938
66792Pasteurella testudinis DSM 23072 strain DSM 230721122938.3wgspatric1122938
66792Pasteurella testudinis strain NCTC12150761.3wgspatric761
66792Pasteurella testudinis NCTC 121502878651225draftimg761
66792Pasteurella testudinis DSM 230722528768223draftimg1122938

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno89.656no
gram-positiveno97.981no
anaerobicno93.424no
aerobicno70.523yes
halophileno59.684no
spore-formingno98.658no
motileno88.78no
thermophileno99.25no
glucose-utilno54.289yes
glucose-fermentno50yes

External links

@ref: 16690

culture collection no.: DSM 23072, ATCC 33688, CCUG 19802, CIP 82.117, NCTC 12150, UCD 90-23-79n

straininfo link

  • @ref: 80959
  • straininfo: 40624

literature

  • topic: Phylogeny
  • Pubmed-ID: 26554864
  • title: Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles.
  • authors: Hansen MJ, Pennanen EAE, Bojesen AM, Christensen H, Bertelsen MF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000759
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Denmark, Fatty Acids/analysis, Fresh Water, Mouth/microbiology, Pasteurellaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Turtles/*microbiology, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16690Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23072)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23072
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38681Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11395
47060Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 19802)https://www.ccug.se/strain?id=19802
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
68369Automatically annotated from API 20NE
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80959Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40624.1StrainInfo: A central database for resolving microbial strain identifiers
123486Curators of the CIPCollection of Institut Pasteur (CIP 82.117)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2082.117