Strain identifier

BacDive ID: 11729

Type strain: Yes

Species: Pasteurella multocida

Strain Designation: W-9217

Strain history: CIP <- 1989, NCTC <- 1962, G.R. Carter, Guelph, Canada: strain W-9217

NCBI tax ID(s): 747 (species)

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General

@ref: 6166

BacDive-ID: 11729

DSM-Number: 16031

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Pasteurella multocida W-9217 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from pig.

NCBI tax id

  • NCBI tax id: 747
  • Matching level: species

strain history

@refhistory
6166<- NCTC <- G. R. Carter; W-9217
121182CIP <- 1989, NCTC <- 1962, G.R. Carter, Guelph, Canada: strain W-9217

doi: 10.13145/bacdive11729.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Pasteurella
  • species: Pasteurella multocida
  • full scientific name: Pasteurella multocida (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Pasteurella gallicida
    20215Bacterium multocidum
    20215Micrococcus gallicidus

@ref: 6166

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Pasteurella

species: Pasteurella multocida subsp. multocida

full scientific name: Pasteurella multocida subsp. multocida (Lehmann and Neumann 1899) Mutters et al. 1985

strain designation: W-9217

type strain: yes

Morphology

cell morphology

  • @ref: 121182
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
61661-2 days
121182

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6166BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
6166COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
32748MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
6166positivegrowth37mesophilic
32748positivegrowth30mesophilic
121182positivegrowth30-37mesophilic
121182nogrowth15psychrophilic
121182nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121182
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
121182NaClpositivegrowth0-4 %
121182NaClnogrowth6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
12118216947citrate-carbon source
12118217234glucose+fermentation
12118217716lactose-fermentation
12118217632nitrate+reduction
12118216301nitrite-reduction
121182132112sodium thiosulfate-builds gas from
6837717634D-glucose+builds acid from

antibiotic resistance

  • @ref: 121182
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
12118235581indoleyes

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
6166catalase+1.11.1.6
6166cytochrome-c oxidase+1.9.3.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
121182oxidase+
121182beta-galactosidase-3.2.1.23
121182alcohol dehydrogenase-1.1.1.1
121182catalase+1.11.1.6
121182lysine decarboxylase-4.1.1.18
121182ornithine decarboxylase+4.1.1.17
121182tryptophan deaminase-
121182urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121182-+-+-------+-+------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6166++------------------+
6166++------------------+
6166++------------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
6166++-----+---+-
6166++-++-++---+-
6166-++-----+---+
6166-++-++-++---+
6166-++-+---+---+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121182++-+---+----------++-+--------------+--+----------------+------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
6166pigCanadaCANNorth America
121182Animal, PigsCanadaCANNorth America1962

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_446.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_298;97_330;98_371;99_446&stattab=map
  • Last taxonomy: Pasteurella multocida subclade
  • 16S sequence: AY078999
  • Sequence Identity:
  • Total samples: 28944
  • soil counts: 516
  • aquatic counts: 911
  • animal counts: 27398
  • plant counts: 119

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61662Risk group (German classification)
1211822Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pasteurella multocida subsp. multocida 16S ribosomal RNA gene, partial sequenceAF2944101364ena44283
20218Pasteurella multocida strain NCTC10322 16S ribosomal RNA gene, partial sequenceAF176289312ena747
20218P.multocida 16S rRNA, V3-V4-V5 variable regions, segment 1M23298100ena747
20218P.multocida 16S rRNA, V3-V4-V5 variable regions, segment 2M23299345ena747
20218P.multocida 16S ribosomal RNAM350181543ena747
6166Pasteurella multocida subsp. multocida strain NCTC 10322 16S ribosomal RNA gene, partial sequenceAY0789991468ena44283

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pasteurella multocida ATCC 43137GCA_000754275completencbi747
66792Pasteurella multocida NCTC10322GCA_900187275completencbi747
66792Pasteurella multocida strain 161215033201-1747.738completepatric747
66792Pasteurella multocida strain ATCC 43137747.1398completepatric747
66792Pasteurella multocida strain NCTC10322747.510completepatric747
66792Pasteurella multocida NCTC 103222858524007completeimg747
66792Pasteurella multocida W-92172597490141completeimg747

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno85.665no
flagellatedno96.238no
gram-positiveno97.887no
anaerobicno97.731no
aerobicno85.476no
halophileno53.351no
spore-formingno98.802no
glucose-utilno61.281yes
thermophileno98.42no
glucose-fermentno53.892yes

External links

@ref: 6166

culture collection no.: DSM 16031, ATCC 43137, NCTC 10322, CIP 103286, CCUG 17976, LMG 2851

straininfo link

  • @ref: 80948
  • straininfo: 3815

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity7714188Proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin.Shryock TR, White DW, Werner CS, Staples JMJ Clin Microbiol10.1128/jcm.33.2.331-335.19951995Animals, *Anti-Bacterial Agents, Cattle, Cattle Diseases/drug therapy, *Guidelines as Topic, *Macrolides, Mannheimia haemolytica/drug effects, Microbial Sensitivity Tests/methods/standards/*veterinary, Pasteurella Infections/drug therapy/veterinary, Pasteurella multocida/drug effects, Quality Control, Reference Standards, Respiratory Tract Infections/drug therapy/veterinary, Staphylococcus aureus/drug effects, Tylosin/*analogs & derivatives/standards/therapeutic use
Enzymology18202950Pasteurella multocida in scavenging family chickens and ducks: carrier status, age susceptibility and transmission between species.Mbuthia PG, Njagi LW, Nyaga PN, Bebora LC, Minga U, Kamundia J, Olsen JEAvian Pathol10.1080/030794507017848912008Aging/*physiology, Animal Husbandry, Animals, Carrier State/microbiology/transmission/*veterinary, Chickens/*microbiology, Ducks/*microbiology, Pasteurella Infections/epidemiology/microbiology/transmission/*veterinary, Pasteurella multocida/*isolation & purification, Poultry Diseases/epidemiology/*microbiology/*transmissionPhylogeny
22107097Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens.Mbuthia PG, Njagi LW, Nyaga PN, Bebora LC, Minga U, Christensen JP, Olsen JEAvian Pathol10.1080/03079457.2011.6232982011Analysis of Variance, Animals, Bacterial Load/veterinary, *Chickens, Cholera/immunology/*veterinary, Dexamethasone, Histological Techniques/veterinary, Immunosuppression Therapy/*veterinary, In Situ Hybridization, Fluorescence/veterinary, Pasteurella Infections/immunology/*veterinary, Pasteurella multocida/*pathogenicity, Poultry Diseases/*immunology/*microbiology, Time Factors
Genetics25342682Complete Genome Sequence of Type Strain Pasteurella multocida subsp. multocida ATCC 43137.Davenport KW, Daligault HE, Minogue TD, Bishop-Lilly KA, Bruce DC, Chain PS, Coyne SR, Frey KG, Jaissle J, Koroleva GI, Ladner JT, Lo CC, Palacios GF, Redden CL, Scholz MB, Teshima H, Johnson SLGenome Announc10.1128/genomeA.01070-142014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6166Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16031)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16031
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32748Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15066
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80948Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3815.1StrainInfo: A central database for resolving microbial strain identifiers
121182Curators of the CIPCollection of Institut Pasteur (CIP 103286)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103286