Strain identifier

BacDive ID: 11724

Type strain: Yes

Species: Mannheimia ruminalis

Strain history: CIP <- 1999, CCUG <- Ö. Angen, SVS, Copenhagen, Denmark: strain HPA92 <- J.E. Phillips

NCBI tax ID(s): 85403 (species)

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General

@ref: 15472

BacDive-ID: 11724

DSM-Number: 21141

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Mannheimia ruminalis DSM 21141 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from sheep rumen.

NCBI tax id

  • NCBI tax id: 85403
  • Matching level: species

strain history

@refhistory
15472<- CIP {2007} <- Ö. Angen <- J.E. Philips
122309CIP <- 1999, CCUG <- Ö. Angen, SVS, Copenhagen, Denmark: strain HPA92 <- J.E. Phillips

doi: 10.13145/bacdive11724.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Mannheimia
  • species: Mannheimia ruminalis
  • full scientific name: Mannheimia ruminalis Angen et al. 1999

@ref: 15472

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Mannheimia

species: Mannheimia ruminalis

full scientific name: Mannheimia ruminalis Angen et al. 1999

type strain: yes

Morphology

cell morphology

  • @ref: 122309
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 122309

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15472COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40279MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicolayesDistilled water make up to (1000.000 ml);Yeast extract (1.000 g);Tryptocasein soy agar (40.000 g)
122309CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b

culture temp

@refgrowthtypetemperaturerange
15472positivegrowth37mesophilic
40279positivegrowth37mesophilic
122309positivegrowth25-37mesophilic
122309nogrowth15psychrophilic
122309nogrowth41thermophilic
122309nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122309
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122309citrate-carbon source16947
122309esculin-hydrolysis4853
122309glucose+fermentation17234
122309hippurate+hydrolysis606565
122309lactose+fermentation17716
122309nitrate+reduction17632
122309nitrite-reduction16301
122309sodium thiosulfate-builds gas from132112
122309glucose-degradation17234
68377D-glucose+builds acid from17634
68377ornithine-degradation18257
68377urea-hydrolysis16199

antibiotic resistance

  • @ref: 122309
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122309
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12230915688acetoin+
12230917234glucose-

enzymes

@refvalueactivityec
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377alkaline phosphatase+3.1.3.1
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
122309oxidase+
122309beta-galactosidase+3.2.1.23
122309alcohol dehydrogenase-1.1.1.1
122309catalase+1.11.1.6
122309gamma-glutamyltransferase-2.3.2.2
122309lysine decarboxylase-4.1.1.18
122309ornithine decarboxylase-4.1.1.17
122309phenylalanine ammonia-lyase-4.3.1.24
122309tryptophan deaminase-
122309urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122309-+---+----++-+------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122309----+----+-+---------+/-------+-+-----------------+

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
15472++-+---++---+
15472+++-+---++---
15472-++-+---++---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
15472sheep rumenUnited KingdomGBREurope
54276Sheep rumenUnited KingdomGBREurope
122309Animal, Sheep, rumenUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Organ#Rumen

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
154721Risk group (German classification)
1223091Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15472
  • description: Mannheimia ruminalis strain HPA92; CCUG 38470 16S ribosomal RNA gene, partial sequence
  • accession: AF053900
  • length: 1504
  • database: ena
  • NCBI tax ID: 85403

External links

@ref: 15472

culture collection no.: DSM 21141, CCUG 38470, CIP 106062, HPA 92

straininfo link

  • @ref: 80943
  • straininfo: 69231

literature

  • topic: Phylogeny
  • Pubmed-ID: 10028248
  • title: Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.
  • authors: Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard M
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-1-67
  • year: 1999
  • mesh: Base Composition, Base Sequence, DNA, Bacterial/*analysis, Mannheimia haemolytica/*classification/genetics, *Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/*chemistry

Reference

@idauthorscataloguedoi/urltitle
15472Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21141)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21141
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40279Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18150
54276Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38470)https://www.ccug.se/strain?id=38470
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
80943Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69231.1StrainInfo: A central database for resolving microbial strain identifiers
122309Curators of the CIPCollection of Institut Pasteur (CIP 106062)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106062