Strain identifier

BacDive ID: 11721

Type strain: Yes

Species: Mannheimia haemolytica

Strain Designation: 1266/56A&B, 1266 A & B

Strain history: CIP <- 1990, ATCC <- NCTC <- 1956, J.A. Watt, Edinburg, UK: strain 1266 A & B

NCBI tax ID(s): 75985 (species)

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General

@ref: 3966

BacDive-ID: 11721

DSM-Number: 10531

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Mannheimia haemolytica 1266/56A&B is a mesophilic, Gram-negative, rod-shaped human pathogen of the family Pasteurellaceae.

NCBI tax id

  • NCBI tax id: 75985
  • Matching level: species

strain history

@refhistory
3966<- ATCC <- NCTC <- J. A. Watt
119765CIP <- 1990, ATCC <- NCTC <- 1956, J.A. Watt, Edinburg, UK: strain 1266 A & B

doi: 10.13145/bacdive11721.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Mannheimia
  • species: Mannheimia haemolytica
  • full scientific name: Mannheimia haemolytica (Newsom and Cross 1932) Angen et al. 1999

  • synonyms

    @refsynonym
    20215Pasteurella hemolytica
    20215Pasteurella haemolytica

@ref: 3966

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Mannheimia

species: Mannheimia haemolytica

full scientific name: Mannheimia haemolytica (Newsom and Cross 1932) Angen et al. 1999

strain designation: 1266/56A&B, 1266 A & B

type strain: yes

Morphology

cell morphology

  • @ref: 119765
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis ability
3966beta1
1197651

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3966COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3966BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
41659MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicolayesDistilled water make up to (1000.000 ml);Yeast extract (1.000 g);Tryptocasein soy agar (40.000 g)
119765CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
119765CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b

culture temp

@refgrowthtypetemperaturerange
3966positivegrowth37mesophilic
41659positivegrowth37mesophilic
119765positivegrowth25-41
119765nogrowth15psychrophilic
119765nogrowth45thermophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
1197654853esculin-hydrolysis
11976517234glucose+fermentation
119765606565hippurate+hydrolysis
11976517716lactose+fermentation
11976517632nitrate+reduction
11976516301nitrite-reduction
119765132112sodium thiosulfate-builds gas from
6837717634D-glucose+builds acid from
6837715824D-fructose+builds acid from
6837717306maltose+builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

antibiotic resistance

  • @ref: 119765
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11976535581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837735581indole-
6836815688acetoin-
6836835581indole-
11976515688acetoin-
11976517234glucose+

enzymes

@refvalueactivityec
3966catalase+1.11.1.6
3966cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
119765oxidase+
119765beta-galactosidase-3.2.1.23
119765alcohol dehydrogenase-1.1.1.1
119765catalase+1.11.1.6
119765gamma-glutamyltransferase-2.3.2.2
119765lysine decarboxylase-4.1.1.18
119765ornithine decarboxylase-4.1.1.17
119765phenylalanine ammonia-lyase-4.3.1.24
119765tryptophan deaminase-
119765urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119765-+---+----++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
3966--------------------+

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3966++++---+-----
3966++++---+-----
3966-++++---+----
3966-++++---+----
3966-++++---+----
3966-++++---+--+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119765------------+-----------------------------------------+++------------------------------------------

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentisolation date
3966EdinburghUnited KingdomGBREurope
1197651956

taxonmaps

  • @ref: 69479
  • File name: preview.99_171.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_125;97_133;98_150;99_171&stattab=map
  • Last taxonomy: Mannheimia haemolytica
  • 16S sequence: NR_114448
  • Sequence Identity:
  • Total samples: 14795
  • soil counts: 383
  • aquatic counts: 410
  • animal counts: 13933
  • plant counts: 69

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3966yesyes2Risk group (German classification)
1197651Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pasteurella haemolytica 16S-23S intergenic spacer region, partial sequence, and tRNA-Glu gene, complete sequenceAF0319361391ena75985
20218Mannheimia haemolytica 16S ribosomal RNA gene, partial sequenceAF0606991504ena75985
20218Mannheimia haemolytica strain NCTC 9380 16S ribosomal RNA gene, partial sequenceM750801492ena75985
3966Mannheimia haemolytica strain NCTC 9380 16S ribosomal RNA, partial sequenceNR_1144481504nuccore75985

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mannheimia haemolytica NCTC9380GCA_900452985contigncbi75985
66792Mannheimia haemolytica strain DSM 1053175985.166wgspatric75985
66792Mannheimia haemolytica strain NCTC938075985.156wgspatric75985
66792Mannheimia haemolytica DSM 105312784746788draftimg75985
66792Mannheimia haemolytica NCTC 93802850321154draftimg75985
66792Mannheimia haemolytica DSM 10531GCA_004342115scaffoldncbi75985

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno89.425no
flagellatedno95.638no
gram-positiveno98.005no
anaerobicno98.419no
aerobicno90.992no
halophileno56.096no
spore-formingno98.425no
glucose-utilno56.969no
thermophileno98.056yes
glucose-fermentyes52.087no

External links

@ref: 3966

culture collection no.: DSM 10531, ATCC 33396, NCTC 9380, CIP 103426, CCM 5141, CCUG 408

straininfo link

  • @ref: 80940
  • straininfo: 40477

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028248Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard MInt J Syst Bacteriol10.1099/00207713-49-1-671999Base Composition, Base Sequence, DNA, Bacterial/*analysis, Mannheimia haemolytica/*classification/genetics, *Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/*chemistry
Phylogeny11702937Characterisation of a novel Mannheimia sp from Australian feedlot cattle.Blackalu P, Angen, Fegan N, Blackall L, Mutters R, Bisgaard MAust Vet J10.1111/j.1751-0813.2001.tb10787.x2001Animals, Australia, Base Composition, Base Sequence, Cattle, Cattle Diseases/*microbiology, DNA, Bacterial/chemistry, Genotype, Nasal Mucosa/microbiology, Nucleic Acid Hybridization, Pasteurella/*classification/genetics/isolation & purification, Pasteurella Infections/microbiology/*veterinary, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Respiratory Tract Infections/microbiology/*veterinary, Ribotyping/veterinaryGenetics
Phylogeny20023064Orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders 'Enterobacteriales' and Pasteurellales.Volkmann M, Skiebe E, Kerrinnes T, Faber F, Lepka D, Pfeifer Y, Holland G, Bannert N, Wilharm GInt J Syst Evol Microbiol10.1099/ijs.0.019026-02009Animals, Base Composition, DNA, Bacterial/genetics, Fatty Acids/metabolism, Feces/*microbiology, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sus scrofa/*microbiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3966Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10531)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10531
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41659Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15222
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68368Automatically annotated from API 20E
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80940Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40477.1StrainInfo: A central database for resolving microbial strain identifiers
119765Curators of the CIPCollection of Institut Pasteur (CIP 103426)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103426