Strain identifier

BacDive ID: 11699

Type strain: No

Species: Haemophilus influenzae

Strain Designation: 644, HK 403

Strain history: CIP <- 1954, M. Pittman, N.I.H.: strain 644, serotype F <- H.K. Kilian: strain HK 403

NCBI tax ID(s): 123833 (strain), 727 (species)

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General

@ref: 3772

BacDive-ID: 11699

DSM-Number: 10000

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Haemophilus influenzae 644 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from blood and spinal fluid.

NCBI tax id

NCBI tax idMatching level
123833strain
727species

strain history

@refhistory
3772<- ATCC <- AMC, 36-A-8 <- M. Pittmann, 644
120107CIP <- 1954, M. Pittman, N.I.H.: strain 644, serotype F <- H.K. Kilian: strain HK 403

doi: 10.13145/bacdive11699.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus influenzae
  • full scientific name: Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Hemophilus influenzae
    20215Bacterium influenzae

@ref: 3772

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus influenzae

full scientific name: Haemophilus influenzae (Lehmann and Neumann 1896) Winslow et al. 1917

strain designation: 644, HK 403

type strain: no

Morphology

cell morphology

  • @ref: 120107
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 3772
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3772HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
3772COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
36178MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
120107CIP Medium 10_BIORADyeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10_BIORAD
120107CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
3772positivegrowth37mesophilic
36178positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3772
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837717634D-glucose+builds acid from
6837717306maltose-builds acid from
6837718257ornithine+degradation

enzymes

@refvalueactivityec
3772catalase+1.11.1.6
3772cytochrome-c oxidase+1.9.3.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377ornithine decarboxylase+4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
44934-+---+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3772++--++-+---+-
3772-++--++-+---+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
3772blood and spinal fluidUSAUSANorth America
44934Human blood & cerebrospinal fluid,meningitidisUSAUSANorth America
120107Cerebrospinal fluidUnited States of AmericaUSANorth America1944

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Blood
#Host Body Product#Fluids#Cerebrospinal fluid

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
37722Risk group (German classification)
1201072Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Haemophilus influenzae ATCC 9833 16S ribosomal RNA gene, partial sequenceAF2243061499ena123833
20218Haemophilus influenzae strain ATCC9833 16S ribosomal RNA gene, partial sequenceAY6137781537ena727

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haemophilus influenzae strain FDAARGOS_1561727.3049completepatric727
66792Haemophilus influenzae strain FDAARGOS_1561727.3040completepatric727

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.675no
flagellatedno96.269no
gram-positiveno97.811no
anaerobicno96.367yes
aerobicno95.343yes
halophileyes56.066no
spore-formingno98.493no
thermophileno98.255no
glucose-utilyes50.542no
glucose-fermentyes57.782no

External links

@ref: 3772

culture collection no.: DSM 10000, ATCC 9833, NCTC 8473, CCUG 6876, AMC 36-A-8, CIP 54.85

straininfo link

  • @ref: 80919
  • straininfo: 46306

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3772Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10000)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10000
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36178Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18312
44934Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 6876)https://www.ccug.se/strain?id=6876
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
80919Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46306.1StrainInfo: A central database for resolving microbial strain identifiers
120107Curators of the CIPCollection of Institut Pasteur (CIP 54.85)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2054.85