Strain identifier

BacDive ID: 11690

Type strain: Yes

Species: Actinobacillus equuli subsp. haemolyticus

Strain Designation: F 154, 8-11272

Strain history: CIP <- 2002, CCUG <- 1986, R. Mutters, Marburg, Germany <- Carman: strain 8-11272

NCBI tax ID(s): 202948 (subspecies)

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General

@ref: 16332

BacDive-ID: 11690

DSM-Number: 22436

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, animal pathogen

description: Actinobacillus equuli subsp. haemolyticus F 154 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from horse.

NCBI tax id

  • NCBI tax id: 202948
  • Matching level: subspecies

strain history

@refhistory
16332<- CCUG <- R. Mutters, Marburg, Germany
119127CIP <- 2002, CCUG <- 1986, R. Mutters, Marburg, Germany <- Carman: strain 8-11272

doi: 10.13145/bacdive11690.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus equuli subsp. haemolyticus
  • full scientific name: Actinobacillus equuli subsp. haemolyticus Christensen et al. 2002

@ref: 16332

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus equuli subsp. haemolyticus

full scientific name: Actinobacillus equuli subsp. haemolyticus Christensen et al. 2002

strain designation: F 154, 8-11272

type strain: yes

Morphology

cell morphology

  • @ref: 119127
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119127
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16332COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39929MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119127CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b
119127CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
16332positivegrowth37mesophilic
39929positivegrowth37mesophilic
119127positivegrowth25-41
119127nogrowth15psychrophilic
119127nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119127
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119127esculin+hydrolysis4853
119127hippurate-hydrolysis606565
119127nitrate+reduction17632
119127nitrite+reduction16301
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377urea+hydrolysis16199
68377tryptophan-energy source27897

antibiotic resistance

  • @ref: 119127
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11912735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837735581indole-
11912715688acetoin-

enzymes

@refvalueactivityec
68377tryptophan deaminase-4.1.99.1
68377alkaline phosphatase+3.1.3.1
68377lipase+
68377urease+3.5.1.5
119127oxidase-
119127beta-galactosidase+3.2.1.23
119127alcohol dehydrogenase-1.1.1.1
119127catalase-1.11.1.6
119127gamma-glutamyltransferase-2.3.2.2
119127lysine decarboxylase-4.1.1.18
119127ornithine decarboxylase-4.1.1.17
119127urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119127-+++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119127----+------------++--+-++++---++------+----------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16332++++-++++-+--
16332-++++-++++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119127++---------++---+-+-----------------------------------------+--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
16332horseNew ZealandNZLAustralia and Oceania
47057HorseNew ZealandNZLAustralia and Oceania1979
119127Animal, HorseNew ZealandNZLAustralia and Oceania1979

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Equidae (Horse)

taxonmaps

  • @ref: 69479
  • File name: preview.99_7618.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_166;98_344;99_7618&stattab=map
  • Last taxonomy: Actinobacillus
  • 16S sequence: M75067
  • Sequence Identity:
  • Total samples: 270
  • soil counts: 23
  • aquatic counts: 1
  • animal counts: 245
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16332yes2Risk group (German classification)
1191271Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus equuli subsp. haemolyticus strain CCUG 19799 16S ribosomal RNA gene, partial sequenceAF3811871362ena202948
20218Actinobacillus sp. CCUG 19799 partial 16S rRNA gene, strain CCUG 19799AJ438657435ena202948
20218Actinobacillus capsulatus strain CCUG 19799 16S ribosomal RNA gene, partial sequenceM750671478ena717
16332Actinobacillus sp. F 154 16S ribosomal RNA gene, partial sequenceAF2477161461ena202948

Genome sequences

  • @ref: 66792
  • description: Actinobacillus equuli subsp. haemolyticus CCUG19799
  • accession: GCA_029773665
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 202948

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno95.079no
gram-positiveno85.874no
anaerobicno85.135no
aerobicno87.857no
halophileno54.512no
spore-formingno98.472no
glucose-fermentyes70.878no
thermophileno94.574no
glucose-utilyes69.774no
motileno85.101no

External links

@ref: 16332

culture collection no.: DSM 22436, CCUG 19799, NCTC 13195, CIP 107709

straininfo link

  • @ref: 80910
  • straininfo: 51091

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361259
  • title: Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov.
  • authors: Christensen H, Bisgaard M, Olsen JE
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1569
  • year: 2002
  • mesh: Actinobacillus/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Horses/*microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16332Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22436)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22436
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39929Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5175
47057Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 19799)https://www.ccug.se/strain?id=19799
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80910Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID51091.1StrainInfo: A central database for resolving microbial strain identifiers
119127Curators of the CIPCollection of Institut Pasteur (CIP 107709)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107709