Strain identifier

BacDive ID: 11686

Type strain: Yes

Species: Actinobacillus scotiae

Strain Designation: M2000/95/1

Strain history: CIP <- 1998, G. Foster, SAC Vet. Services, Inverness, UK: strain M2000/95/1

NCBI tax ID(s): 182182 (species)

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General

@ref: 16201

BacDive-ID: 11686

DSM-Number: 22205

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped, animal pathogen

description: Actinobacillus scotiae M2000/95/1 is a microaerophile, mesophilic, Gram-negative animal pathogen that was isolated from viscera of porpoises .

NCBI tax id

  • NCBI tax id: 182182
  • Matching level: species

strain history

@refhistory
16201<- CCUG <- B. Holmes, NCTC <- ? <- G. Foster, SAC Veterinary Services, Inverness, United Kingdom; M2000/95/1
372191998, G. Foster, SAC Vet. Services, Inverness, UK: strain M2000/95/1
122527CIP <- 1998, G. Foster, SAC Vet. Services, Inverness, UK: strain M2000/95/1

doi: 10.13145/bacdive11686.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus scotiae
  • full scientific name: Actinobacillus scotiae Foster et al. 1998

@ref: 16201

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus scotiae

full scientific name: Actinobacillus scotiae Foster et al. 1998

strain designation: M2000/95/1

type strain: yes

Morphology

cell morphology

  • @ref: 122527
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis ability
16201gamma
1225271

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16201COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37219MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
122527CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
16201positivegrowth37mesophilic
37219positivegrowth37mesophilic
57177positivegrowth37mesophilic
122527positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16201microaerophile
57177microaerophile
122527facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1225274853esculin-hydrolysis
122527606565hippurate-hydrolysis
12252717632nitrate+reduction
12252716301nitrite-reduction
6837717306maltose-builds acid from
6837718257ornithine+degradation
6837716199urea+hydrolysis
6837727897tryptophan-energy source

antibiotic resistance

  • @ref: 122527
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12252735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837735581indole-
12252715688acetoin+

enzymes

@refvalueactivityec
16201catalase-1.11.1.6
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377urease+3.5.1.5
68377ornithine decarboxylase+4.1.1.17
122527oxidase-
122527beta-galactosidase+3.2.1.23
122527alcohol dehydrogenase-1.1.1.1
122527catalase-1.11.1.6
122527gamma-glutamyltransferase+2.3.2.2
122527lysine decarboxylase-4.1.1.18
122527ornithine decarboxylase+4.1.1.17
122527urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122527-+++-+----++-+------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16201++/---+++-+----
16201-++/---+++-+---

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling dateisolation date
16201viscera of porpoises (Phocoena phocoena)Phocoena phocoenaScottish coastlineUnited KingdomGBREurope
57177Porpoise visceraNorth Sea1995
122527Porpoise, visceraNorth Sea, ScotlandUnited KingdomGBREurope1995

isolation source categories

Cat1Cat2
#Host#Mammals
#Host Body-Site#Organ

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16201yes2Risk group (German classification)
1225271Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16201
  • description: Actinobacillus scotiae 16S rRNA gene, strain M2000/95/1
  • accession: Y09653
  • length: 1497
  • database: ena
  • NCBI tax ID: 182182

GC content

  • @ref: 16201
  • GC-content: 40.5

External links

@ref: 16201

culture collection no.: DSM 22205, CCUG 46379, CIP 105697, NCTC 12922

straininfo link

  • @ref: 80906
  • straininfo: 44155

literature

  • topic: Phylogeny
  • Pubmed-ID: 9734048
  • title: Actinobacillus scotiae sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from porpoises (Phocoena phocoena).
  • authors: Foster G, Ross HM, Patterson IA, Hutson RA, Collins MD
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-48-3-929
  • year: 1998
  • mesh: Actinobacillus/*classification, Animals, Base Sequence, Molecular Sequence Data, Phylogeny, Porpoises/*microbiology, RNA, Ribosomal, 16S/chemistry
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
16201Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22205)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22205
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37219Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17745
57177Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46379)https://www.ccug.se/strain?id=46379
68377Automatically annotated from API NH
68382Automatically annotated from API zym
80906Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44155.1StrainInfo: A central database for resolving microbial strain identifiers
122527Curators of the CIPCollection of Institut Pasteur (CIP 105697)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105697