Strain identifier

BacDive ID: 11685

Type strain: Yes

Species: Actinobacillus indolicus

Strain Designation: 46KC2

Strain history: CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain 46KC2 <- K. Moeller

NCBI tax ID(s): 51049 (species)

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General

@ref: 16244

BacDive-ID: 11685

DSM-Number: 22264

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Actinobacillus indolicus 46KC2 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from pig upper respiratory tract.

NCBI tax id

  • NCBI tax id: 51049
  • Matching level: species

strain history

@refhistory
16244<- CCUG <- K. Møller, Danish Vet. Lab., Copenhagen, Denmark
120091CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain 46KC2 <- K. Moeller

doi: 10.13145/bacdive11685.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus indolicus
  • full scientific name: Actinobacillus indolicus Møller et al. 1996

@ref: 16244

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus indolicus

full scientific name: Actinobacillus indolicus Møller et al. 1996

strain designation: 46KC2

type strain: yes

Morphology

cell morphology

  • @ref: 120091
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16244HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
39260MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
120091CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
16244positivegrowth37mesophilic
39260positivegrowth37mesophilic
120091positivegrowth25-41
120091nogrowth15psychrophilic
120091nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120091
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371xylitol-builds acid from17151
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
120091esculin-hydrolysis4853
120091hippurate-hydrolysis606565
120091nitrate+reduction17632
120091nitrite-reduction16301
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377ornithine-degradation18257
68377urea-hydrolysis16199

antibiotic resistance

  • @ref: 120091
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120091
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
68377alkaline phosphatase+3.1.3.1
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase+3.5.2.6
120091oxidase-
120091beta-galactosidase+3.2.1.23
120091alcohol dehydrogenase-1.1.1.1
120091catalase+1.11.1.6
120091gamma-glutamyltransferase-2.3.2.2
120091lysine decarboxylase-4.1.1.18
120091ornithine decarboxylase-4.1.1.17
120091urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120091-+--------++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120091+/----+/------+/-+/-+--------+/-+/-+++-+--++--++/-+/--+/-+---+--+--

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16244++++---++-+-+
16244+++++---++-+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
16244pig upper respiratory tract
54624Pig upper respiratory tract
120091Animal, Pig, respiratory tractDenmarkDNKEurope1990

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Oral cavity and airways#Airways

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
162441Risk group (German classification)
1200911Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16244
  • description: Actinobacillus indolicus strain 46KC2 16S ribosomal RNA gene, partial sequence
  • accession: AY362891
  • length: 1362
  • database: ena
  • NCBI tax ID: 51049

External links

@ref: 16244

culture collection no.: DSM 22264, CCUG 39029, CIP 105316

straininfo link

  • @ref: 80905
  • straininfo: 51110

literature

  • topic: Phylogeny
  • Pubmed-ID: 8863422
  • title: Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.
  • authors: Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian M
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-46-4-951
  • year: 1996
  • mesh: Actinobacillus/*classification, Animals, Base Sequence, DNA, Bacterial/analysis, DNA, Ribosomal/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Respiratory System/*microbiology, Swine/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
16244Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22264)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22264
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39260Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17322
54624Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39029)https://www.ccug.se/strain?id=39029
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
80905Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID51110.1StrainInfo: A central database for resolving microbial strain identifiers
120091Curators of the CIPCollection of Institut Pasteur (CIP 105316)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105316