Strain identifier

BacDive ID: 11682

Type strain: Yes

Species: Actinobacillus suis

Strain Designation: 1276/61

Strain history: CIP <- 1989, CCM <- O. Mràz, Actinobacillus equuli <- C.A. van Dorssen: strain 1276/61

NCBI tax ID(s): 743972 (strain), 716 (species)

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General

@ref: 16330

BacDive-ID: 11682

DSM-Number: 22433

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, human pathogen

description: Actinobacillus suis 1276/61 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from porcine, septicaemia.

NCBI tax id

NCBI tax idMatching level
716species
743972strain

strain history

@refhistory
16330<- CCUG; CCUG 11624 <- W. Frederiksen, SSIC <- C. A. van Dorssen
123375CIP <- 1989, CCM <- O. Mràz, Actinobacillus equuli <- C.A. van Dorssen: strain 1276/61

doi: 10.13145/bacdive11682.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus suis
  • full scientific name: Actinobacillus suis van Dorssen and Jaartsveld 1962 (Approved Lists 1980)

@ref: 16330

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus suis

full scientific name: Actinobacillus suis van Dorssen and Jaartsveld 1962

strain designation: 1276/61

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.982
123375negativerod-shapedno

colony morphology

@reftype of hemolysishemolysis ability
16330alpha/beta1
1233751

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16330COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40618MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serumyesHorse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml)
123375CIP Medium 4yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4

culture temp

@refgrowthtypetemperaturerange
16330positivegrowth37mesophilic
40618positivegrowth37mesophilic
45577positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
45577microaerophile
123375obligate aerobe

spore formation

@refspore formationconfidence
69481no96
69480no99.994

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea+hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
123375esculin+hydrolysis4853
123375nitrate+reduction17632
123375nitrite+reduction16301
68374ornithine-degradation18257
68374arginine-hydrolysis29016
68374lysine-degradation25094
68374urea+hydrolysis16199
68374L-arabitol-builds acid from18403
68374D-galacturonic acid-builds acid from18024
68374Potassium 5-ketogluconate-builds acid from
68374D-mannitol-builds acid from16899
68374maltose-builds acid from17306
68374ribitol-builds acid from15963
68374palatinose+builds acid from18394
68374malonate-assimilation15792
68374tryptophan-energy source27897
68374D-glucose-builds acid from17634
68374sucrose-builds acid from17992
68374L-arabinose-builds acid from30849
68374D-arabitol-builds acid from18333
68374trehalose-builds acid from27082
68374L-rhamnose-builds acid from62345
68374myo-inositol-builds acid from17268
68374cellobiose-builds acid from17057
68374sorbitol-builds acid from30911
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377urea+hydrolysis16199
68377tryptophan-energy source27897

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6837435581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12337535581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837735581indole-
6837435581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
16330catalase+1.11.1.6
16330cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377alkaline phosphatase+3.1.3.1
68377lipase+
68377urease+3.5.1.5
68374L-aspartate arylamidase+3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase+3.2.1.21
68374lipase+
68374urease+3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase-4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
123375oxidase-
123375alcohol dehydrogenase-1.1.1.1
123375catalase+1.11.1.6
123375lysine decarboxylase-4.1.1.18
123375ornithine decarboxylase-4.1.1.17
123375urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123375-+++------++-++-----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
45577+-----+--------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123375+--+++---++++--------+-+++++++++--+---+-------+/---

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
45577---+---+-+---+----+-----+------+

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16330++++-++++-+--
16330-++++-++++-+-

Isolation, sampling and environmental information

isolation

@refsample typesampling date
16330porcine, septicaemia
45577Porcine,septicaemia1961
123375Animal, Pig, blood

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_414.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_166;98_344;99_414&stattab=map
  • Last taxonomy: Actinobacillus suis
  • 16S sequence: AY362899
  • Sequence Identity:
  • Total samples: 1356
  • soil counts: 7
  • aquatic counts: 45
  • animal counts: 1302
  • plant counts: 2

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
16330yesyes2Risk group (German classification)
1233751Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus suis partial 16S rRNA gene, strain P635AJ438660435ena716
16330Actinobacillus suis ATCC 33415 16S ribosomal RNA gene, partial sequenceAY3628991362ena743972

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus suis ATCC 33415GCA_000739435completencbi743972
66792Actinobacillus suis NCTC12996GCA_900187155completencbi716
66792Actinobacillus suis ATCC 33415743972.3completepatric743972
66792Actinobacillus suis strain NCTC12996716.12completepatric716
66792Actinobacillus suis NCTC 129962773858066completeimg716
66792Actinobacillus suis ATCC 334152597489880completeimg743972

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileno90.33no
flagellatedno93.991no
gram-positiveno98.081no
anaerobicno97.167no
aerobicno89.163yes
halophileno61.949no
spore-formingno98.547no
thermophileno99.053yes
glucose-utilno53.148no
glucose-fermentno55.473yes

External links

@ref: 16330

culture collection no.: DSM 22433, ATCC 33415, CCM 5586, CCUG 11624, CIP 103354, NCTC 12996

straininfo link

  • @ref: 80902
  • straininfo: 126053

literature

topicPubmed-IDtitleauthorsjournalDOIyearmesh
Phylogeny11081581Detection and subtyping of Actinobacillus pleuropneumoniae strains by PCR-RFLP analysis of the tbpA and tbpB genes.de la Puente-Redondo VA, del Blanco NG, Gutierrez-Martin CB, Mendez JN, Rodriquez Ferri EFRes Microbiol10.1016/s0923-2508(00)00135-22000Actinobacillus pleuropneumoniae/*classification/genetics, Animals, Carrier Proteins/*genetics, Iron-Binding Proteins, *Polymerase Chain Reaction, *Polymorphism, Restriction Fragment Length, Swine, Transferrin-Binding Proteins
Genetics25237027Genome Sequence of Actinobacillus suis Type Strain ATCC 33415T.Calcutt MJ, Foecking MF, Mhlanga-Mutangadura T, Reilly TJGenome Announc10.1128/genomeA.00926-142014
Phylogeny36213600Actinobacillus suis isolated from diseased pigs are phylogenetically related but harbour different number of toxin gene copies in their genomes.Kulathunga DGRS, Fakher AA, Costa MOVet Rec Open10.1002/vro2.452022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16330Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22433)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22433
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40618Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15142
45577Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 11624)https://www.ccug.se/strain?id=11624
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68374Automatically annotated from API ID32E
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80902Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID126053.1StrainInfo: A central database for resolving microbial strain identifiers
123375Curators of the CIPCollection of Institut Pasteur (CIP 103354)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103354