Strain identifier

BacDive ID: 11676

Type strain: Yes

Species: Actinobacillus capsulatus

Strain Designation: 11-61, P243

Strain history: CIP <- 1989, NCTC <- 1981, W. Frederiksen, Stat. Ser., Copenhagen, Denmark: strain P243 <- 1963, S.N. Arseculeratne: strain 11-61

NCBI tax ID(s): 1120931 (strain), 717 (species)

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General

@ref: 8301

BacDive-ID: 11676

DSM-Number: 19761

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, animal pathogen

description: Actinobacillus capsulatus 11-61 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from rabbit joint.

NCBI tax id

NCBI tax idMatching level
717species
1120931strain

strain history

@refhistory
8301<- CIP <- NCTC <- W. Frederiksen; P243 <- S. N. Arseculeratne; 11-61
117176CIP <- 1989, NCTC <- 1981, W. Frederiksen, Stat. Ser., Copenhagen, Denmark: strain P243 <- 1963, S.N. Arseculeratne: strain 11-61

doi: 10.13145/bacdive11676.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus capsulatus
  • full scientific name: Actinobacillus capsulatus Arseculeratne 1962 (Approved Lists 1980)

@ref: 8301

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus capsulatus

full scientific name: Actinobacillus capsulatus Arseculeratne 1962 emend. Kuhnert et al. 2007

strain designation: 11-61, P243

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shape
69480negative99.955
117176negativerod-shaped

colony morphology

@reftype of hemolysis
8301gamma
117176

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8301BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
8301COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40096MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
117176CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
117176CIP Medium 118-byeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=118-b

culture temp

@refgrowthtypetemperaturerange
8301positivegrowth37mesophilic
40096positivegrowth37mesophilic
117176positivegrowth30-41
117176nogrowth15psychrophilic
117176nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117176
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.995

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117176nitrate+reduction17632
117176nitrite-reduction16301
117176sodium thiosulfate-builds gas from132112
68377D-glucose+builds acid from17634
68377D-fructose+builds acid from15824
68377maltose+builds acid from17306
68377tryptophan-energy source27897

antibiotic resistance

  • @ref: 117176
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11717635581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837735581indole-
11717615688acetoin+
11717617234glucose-

enzymes

@refvalueactivityec
8301cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377alkaline phosphatase+3.1.3.1
117176oxidase+
117176beta-galactosidase+3.2.1.23
117176alcohol dehydrogenase-1.1.1.1
117176gelatinase-
117176catalase+1.11.1.6
117176lysine decarboxylase-4.1.1.18
117176ornithine decarboxylase-4.1.1.17
117176urease+3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117176---+++---++++/-----++----++/-+/--+++++--+++--+------+/---

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
8301++++-++/-++----
8301-++++-++/-++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117176++-+--++++++-----+++++-----------+--+------------++--++-----+--------------+----+-+------+----+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8301rabbit jointSri LankaLKAAsia
45688Rabbit joint (hare arthritis ?)
117176Animal, Rabbit

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Leporidae (Rabbit/Hare)
#Host Body-Site#Limb#Joint

taxonmaps

  • @ref: 69479
  • File name: preview.99_137259.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_50187;97_64609;98_91465;99_137259&stattab=map
  • Last taxonomy: Actinobacillus capsulatus subclade
  • 16S sequence: AY362886
  • Sequence Identity:
  • Total samples: 139
  • aquatic counts: 3
  • animal counts: 136

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
8301yes2Risk group (German classification)
1171761Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus capsulatus partial 16S rRNA gene, strain P243AJ438675435ena717
20218Actinobacillus capsulatus strain NCTC 11408 16S ribosomal RNA gene, partial sequenceM750621476ena717
20218Actinobacillus capsulatus strain CCUG 12396 16S ribosomal RNA gene, partial sequenceM750691476ena717
8301Actinobacillus capsulatus strain CCUG 12396 16S ribosomal RNA gene, partial sequenceAY3628861362ena717

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus capsulatus DSM 19761GCA_000374285scaffoldncbi1120931
66792Actinobacillus capsulatus DSM 197611120931.3wgspatric1120931
66792Actinobacillus capsulatus DSM 197612519899771draftimg1120931

GC content

  • @ref: 8301
  • GC-content: 42.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.846no
flagellatedno95.098no
gram-positiveno97.956no
anaerobicno98.093no
aerobicno85.538no
halophileno60.452no
spore-formingno98.477no
glucose-utilyes54.144no
thermophileno98.838yes
glucose-fermentno50no

External links

@ref: 8301

culture collection no.: DSM 19761, ATCC 51571, CCUG 12396, CIP 103283, NCTC 11408

straininfo link

  • @ref: 80896
  • straininfo: 45302

literature

  • topic: Phylogeny
  • Pubmed-ID: 17329797
  • title: Emended description of Actinobacillus capsulatus Arseculeratne 1962, 38AL.
  • authors: Kuhnert P, Korczak BM, Christensen H, Bisgaard M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64659-0
  • year: 2007
  • mesh: Actinobacillus/*classification/genetics, Actinobacillus Infections/microbiology, Animals, Cricetinae, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Rabbits

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8301Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19761)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19761
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40096Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15063
45688Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 12396)https://www.ccug.se/strain?id=12396
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80896Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45302.1StrainInfo: A central database for resolving microbial strain identifiers
117176Curators of the CIPCollection of Institut Pasteur (CIP 103283)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103283