Strain identifier

BacDive ID: 11670

Type strain: Yes

Species: Actinobacillus ureae

Strain Designation: 3520/59

Strain history: CIP <- 1962, S.D. Henriksen, State Inst. of Public Health, Oslo, Norway: strain 3520/59, Pasteurella haemolytica var. ureae

NCBI tax ID(s): 887324 (strain), 723 (species)

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General

@ref: 2224

BacDive-ID: 11670

DSM-Number: 5568

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Actinobacillus ureae 3520/59 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from nose swab.

NCBI tax id

NCBI tax idMatching level
723species
887324strain

strain history

@refhistory
2224<- ATCC (Pasteurella ureae) <- S.D. Henriksen; 3520/59
376081962, S.D. Henriksen, State Inst. of Public Health, Oslo, Norway: strain 3520/59, Pasteurella haemolytica var. ureae
123738CIP <- 1962, S.D. Henriksen, State Inst. of Public Health, Oslo, Norway: strain 3520/59, Pasteurella haemolytica var. ureae

doi: 10.13145/bacdive11670.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus ureae
  • full scientific name: Actinobacillus ureae (Jones 1962) Mutters et al. 1986

  • synonyms

    • @ref: 20215
    • synonym: Pasteurella ureae

@ref: 2224

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus ureae

full scientific name: Actinobacillus ureae (Jones 1962) Mutters et al. 1986

strain designation: 3520/59

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.985
123738negativerod-shapedno

colony morphology

  • @ref: 123738
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2224COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
37608MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123738CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
2224positivegrowth37mesophilic
37608positivegrowth37mesophilic
44498positivegrowth37mesophilic
123738positivegrowth25-37mesophilic
123738nogrowth15psychrophilic
123738nogrowth41thermophilic
123738nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44498
  • oxygen tolerance: microaerophile

antibiogram

  • @ref: 2224
  • medium: M 693
  • Penicillin G: 34
  • oxacillin: 16-18
  • ampicillin: 34
  • ticarcillin: 40
  • mezlocillin: 36-38
  • cefalotin: 38
  • cefazolin: 32
  • cefotaxime: 44-46
  • aztreonam: 42
  • imipenem: 30
  • tetracycline: 28
  • chloramphenicol: 36-38
  • gentamycin: 14
  • amikacin: 16
  • vancomycin: 0
  • erythromycin: 24-26
  • lincomycin: 14-16
  • ofloxacin: 30
  • norfloxacin: 28
  • colistin: 14-16
  • pipemidic acid: 20-22
  • nitrofurantoin: 30
  • bacitracin: 12-14
  • polymyxin b: 16-18
  • kanamycin: 26
  • neomycin: 12
  • doxycycline: 26
  • ceftriaxone: 42
  • clindamycin: 22
  • fosfomycin: 24
  • moxifloxacin: 30
  • linezolid: 10-12
  • nystatin: 0
  • quinupristin/dalfopristin: 16-18
  • teicoplanin: 0
  • piperacillin/tazobactam: 38

spore formation

@refspore formationconfidence
69481no97
69480no99.997

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123738esculin-hydrolysis4853
123738hippurate+hydrolysis606565
123738nitrate+reduction17632
123738nitrite-reduction16301

antibiotic resistance

  • @ref: 123738
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 123738
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123738oxidase+
123738beta-galactosidase-3.2.1.23
123738alcohol dehydrogenase-1.1.1.1
123738catalase-1.11.1.6
123738gamma-glutamyltransferase-2.3.2.2
123738lysine decarboxylase-4.1.1.18
123738ornithine decarboxylase-4.1.1.17
123738tryptophan deaminase-
123738urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123738-+---+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123738----+/------++/------+------------+/-------------------

Isolation, sampling and environmental information

isolation

@refsample typesampling datecountryorigin.countrycontinentisolation date
2224nose swab
44498Human nose,46-yr-old man living in rural disctrict1959NorwayNOREurope
123738Nasal swabNorwayNOREurope1959

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Swab
#Host Body-Site#Organ#Nose

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
22242Risk group (German classification)
1237381Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinobacillus ureae strain Henrikson 3520/59 16S ribosomal RNA gene, partial sequenceM750751478ena723
20218Actinobacillus ureae strain CCUG 2139 16S ribosomal RNA gene, partial sequenceAY3629001362ena887324
20218Actinobacillus ureae partial 16S rRNA gene, strain P161AJ438663435ena723

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus ureae ATCC 25976GCA_000188255scaffoldncbi887324
66792Actinobacillus ureae ATCC 25976887324.3wgspatric887324
66792Actinobacillus ureae ATCC 25976649989900draftimg887324

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno97no
motileno89.764no
flagellatedno94.405no
gram-positiveno98.305no
anaerobicno97.89no
aerobicno86.978no
halophileno50no
spore-formingno98.673no
glucose-utilno55.868no
thermophileno99.332yes
glucose-fermentno51.958no

External links

@ref: 2224

culture collection no.: DSM 5568, ATCC 25976, NCTC 10219, CCUG 2139, CIP 62.8, CCM 5774

straininfo link

  • @ref: 80891
  • straininfo: 38738

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2224Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5568)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5568
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37608Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9953
44498Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2139)https://www.ccug.se/strain?id=2139
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80891Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38738.1StrainInfo: A central database for resolving microbial strain identifiers
123738Curators of the CIPCollection of Institut Pasteur (CIP 62.8)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2062.8