Strain identifier

BacDive ID: 11606

Type strain: Yes

Species: Paenibacillus dendritiformis

Strain Designation: T168

Strain history: CIP <- 1999, D. Gutnick, Tel Aviv Univ., Tel Aviv, Israel: strain T168 <- Bacillus Gen. Stock Center, Columbus, Ohio, USA: strain BGSC 30A1T

NCBI tax ID(s): 130049 (species)

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General

@ref: 7737

BacDive-ID: 11606

DSM-Number: 18844

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Paenibacillus dendritiformis T168 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from contamination of Bacillus subtilis 168 stock.

NCBI tax id

  • NCBI tax id: 130049
  • Matching level: species

strain history

@refhistory
7737<- CIP <- D. L. Gutnick; T168 <- Bacillus Gen. Stock Center, Columbus, USA; strain BGSC 30A1
118890CIP <- 1999, D. Gutnick, Tel Aviv Univ., Tel Aviv, Israel: strain T168 <- Bacillus Gen. Stock Center, Columbus, Ohio, USA: strain BGSC 30A1T

doi: 10.13145/bacdive11606.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus dendritiformis
  • full scientific name: Paenibacillus dendritiformis Tcherpakov et al. 1999

@ref: 7737

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus dendritiformis

full scientific name: Paenibacillus dendritiformis Tcherpakov et al. 1999

strain designation: T168

type strain: yes

Morphology

cell morphology

  • @ref: 118890
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118890
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7737CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
39411MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
118890CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
7737positivegrowth30mesophilic
39411positivegrowth37mesophilic
118890positivegrowth22-45
118890nogrowth10psychrophilic
118890nogrowth55thermophilic

culture pH

  • @ref: 118890
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 118890
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 118890
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
118890NaClpositivegrowth0-4 %
118890NaClnogrowth6 %
118890NaClnogrowth8 %
118890NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
118890citrate-carbon source16947
118890esculin+hydrolysis4853
118890hippurate+hydrolysis606565
118890nitrate-reduction17632
118890nitrite-reduction16301
118890nitrate+respiration17632

metabolite production

  • @ref: 118890
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11889015688acetoin-
11889017234glucose+

enzymes

@refvalueactivityec
118890oxidase+
118890beta-galactosidase+3.2.1.23
118890alcohol dehydrogenase-1.1.1.1
118890gelatinase+
118890amylase-
118890DNase-
118890caseinase-3.4.21.50
118890catalase+1.11.1.6
118890tween esterase-
118890gamma-glutamyltransferase-2.3.2.2
118890lecithinase-
118890lipase-
118890lysine decarboxylase-4.1.1.18
118890ornithine decarboxylase-4.1.1.17
118890protease+
118890urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118890-+++------++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118890+---+--+--+++-------++++++++/--+++--+++-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118890+-+++-+++++-----++++--+--+-----++-++-+---+-------++--++---+++-----------------+++-+-+-+-+-+---+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7737contamination of Bacillus subtilis 168 stockIsraelISRAsia
118890Contamination of Bacillus subtilis 168 stockIsraelISRAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Engineered#Laboratory#Lab enrichment
#Host#Microbial#Bacteria

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
77371Risk group (German classification)
1188901Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paenibacillus dendritiformis 16S ribosomal RNA gene, partial sequenceAY3598851471ena130049
7737Paenibacillus sp. T-168 16S rRNA geneY161281490ena58172

External links

@ref: 7737

culture collection no.: DSM 18844, CIP 105967, BGSC 30A1, BGSC 30A1T

straininfo link

  • @ref: 80832
  • straininfo: 69554

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7737Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18844)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18844
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39411Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18045
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
80832Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69554.1StrainInfo: A central database for resolving microbial strain identifiers
118890Curators of the CIPCollection of Institut Pasteur (CIP 105967)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105967