Strain identifier
BacDive ID: 11575
Type strain:
Species: Paenibacillus anaericanus
Strain Designation: MH21
Strain history: CIP <- 2007, DSMZ <- M. Horn, Bayreuth Univ., Bayreuth, Germany: strain MH21
NCBI tax ID(s): 170367 (species)
General
@ref: 6140
BacDive-ID: 11575
DSM-Number: 15890
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped
description: Paenibacillus anaericanus MH21 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from earthworm gut.
NCBI tax id
- NCBI tax id: 170367
- Matching level: species
strain history
@ref | history |
---|---|
6140 | <- M. Horn; MH21 |
116369 | CIP <- 2007, DSMZ <- M. Horn, Bayreuth Univ., Bayreuth, Germany: strain MH21 |
doi: 10.13145/bacdive11575.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Paenibacillaceae
- genus: Paenibacillus
- species: Paenibacillus anaericanus
- full scientific name: Paenibacillus anaericanus Horn et al. 2005
synonyms
- @ref: 20215
- synonym: Paenibacillus ginsengisoli
@ref: 6140
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Paenibacillaceae
genus: Paenibacillus
species: Paenibacillus anaericanus
full scientific name: Paenibacillus anaericanus Horn et al. 2005 emend. Kim et al. 2011
strain designation: MH21
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
31407 | negative | 3.5 µm | 0.75 µm | rod-shaped | yes | |
69480 | yes | 97.877 | ||||
69480 | positive | 100 | ||||
116369 | rod-shaped | no |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6140 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Soil extract 500.0 g/l Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
36815 | MEDIUM 328- for nutrient agar | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g) | |
116369 | CIP Medium 328 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6140 | positive | growth | 30 | mesophilic |
31407 | positive | growth | 5.0-40 | |
31407 | positive | optimum | 32.5 | mesophilic |
36815 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31407 | positive | growth | 5.8-8.5 | alkaliphile |
31407 | positive | optimum | 7.7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
31407 | facultative anaerobe |
116369 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
31407 | yes | |
69481 | yes | 100 |
69480 | yes | 100 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31407 | NaCl | positive | growth | 0.0-2.0 % |
31407 | NaCl | positive | optimum | 1 % |
observation
- @ref: 31407
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31407 | 22599 | arabinose | + | carbon source |
31407 | 17057 | cellobiose | + | carbon source |
31407 | 28757 | fructose | + | carbon source |
31407 | 17234 | glucose | + | carbon source |
31407 | 17754 | glycerol | + | carbon source |
31407 | 17716 | lactose | + | carbon source |
31407 | 17306 | maltose | + | carbon source |
31407 | 29864 | mannitol | + | carbon source |
31407 | 37684 | mannose | + | carbon source |
31407 | 506227 | N-acetylglucosamine | + | carbon source |
31407 | 16634 | raffinose | + | carbon source |
31407 | 18222 | xylose | + | carbon source |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | - | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | - | builds acid from |
68371 | 28066 | gentiobiose | - | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | + | builds acid from |
68371 | 28017 | starch | + | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | + | builds acid from |
68371 | 17992 | sucrose | - | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | + | builds acid from |
68371 | 17306 | maltose | + | builds acid from |
68371 | 17057 | cellobiose | + | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | + | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | - | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 17634 | D-glucose | - | builds acid from |
68371 | 12936 | D-galactose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | - | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
116369 | 17632 | nitrate | - | reduction |
116369 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 116369
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
31407 | catalase | + | 1.11.1.6 |
116369 | oxidase | + | |
116369 | catalase | + | 1.11.1.6 |
116369 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116369 | - | - | + | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116369 | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | + | + | + | - | - | + | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent |
---|---|---|---|---|---|---|
6140 | earthworm (Aporrectodea caliginosa) gut | Aporrectodea caliginosa | Bavaria, Bayreuth | Germany | DEU | Europe |
116369 | Animal, Gut of earthworm, Aporrectodea caliginosa | Bayreuth | Germany | DEU | Europe |
isolation source categories
- Cat1: #Host
- Cat2: #Invertebrates (Other)
- Cat3: #Annelida
taxonmaps
- @ref: 69479
- File name: preview.99_82263.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_18589;97_22986;98_28980;99_82263&stattab=map
- Last taxonomy: Paenibacillus anaericanus
- 16S sequence: FN673694
- Sequence Identity:
- Total samples: 63
- soil counts: 45
- aquatic counts: 3
- animal counts: 12
- plant counts: 3
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6140 | 1 | Risk group (German classification) |
116369 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Paenibacillus anaericanus partial 16S rRNA gene, strain DSM 15890 | FN673694 | 1475 | ena | 170367 |
6140 | Earthworm gut bacterium MH21 partial 16S rRNA gene | AJ318909 | 1512 | ena | 170367 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Paenibacillus anaericanus DSM 15890 | GCA_003994475 | scaffold | ncbi | 170367 |
66792 | Paenibacillus anaericanus strain DSM 15890 | 170367.3 | wgs | patric | 170367 |
66792 | Paenibacillus anaericanus DSM 15890 | 2916373010 | draft | img | 170367 |
GC content
- @ref: 6140
- GC-content: 42.6
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 87.511 | yes |
flagellated | yes | 77.136 | no |
gram-positive | yes | 83.034 | no |
anaerobic | no | 96.411 | no |
aerobic | yes | 52.952 | yes |
halophile | no | 94.184 | yes |
spore-forming | yes | 96.559 | no |
thermophile | no | 99.678 | yes |
glucose-util | yes | 84.019 | no |
glucose-ferment | no | 83.289 | no |
External links
@ref: 6140
culture collection no.: DSM 15890, ATCC BAA 844, CIP 109447, KCTC 13749
straininfo link
- @ref: 80803
- straininfo: 138998
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15879265 | Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. | Horn MA, Ihssen J, Matthies C, Schramm A, Acker G, Drake HL | Int J Syst Evol Microbiol | 10.1099/ijs.0.63484-0 | 2005 | Aerobiosis, Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Betaproteobacteria/*classification/cytology/isolation & purification/physiology, Cytochromes c/isolation & purification, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Digestive System/microbiology, Endospore-Forming Bacteria/*classification/cytology/isolation & purification/physiology, Flavobacterium/*classification/cytology/isolation & purification/physiology, Genes, rRNA, Gentian Violet, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Nitrates/metabolism, Nitrogen/metabolism, Nitrous Oxide/*metabolism, Oligochaeta/*microbiology, Oxidation-Reduction, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature | Enzymology |
Phylogeny | 17043912 | Paenibacillus ginsengisoli sp. nov., a novel bacterium isolated from soil of a ginseng field in Pocheon Province, South Korea. | Lee M, Ten LN, Baek SH, Im WT, Aslam Z, Lee ST | Antonie Van Leeuwenhoek | 10.1007/s10482-006-9102-x | 2007 | Aerobiosis, Anaerobiosis, Bacterial Typing Techniques, Base Composition, Carbon/metabolism, Catalase/analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Gram-Positive Endospore-Forming Bacteria/chemistry/*classification/*isolation & purification/physiology, Korea, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Oxidoreductases/analysis, Panax, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology | Enzymology |
Phylogeny | 20870883 | Reclassification of Paenibacillus ginsengisoli as a later heterotypic synonym of Paenibacillus anaericanus. | Kim KK, Lee KC, Lee JS | Int J Syst Evol Microbiol | 10.1099/ijs.0.025650-0 | 2010 | Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
Phylogeny | 24827707 | Paenibacillus selenii sp. nov., isolated from selenium mineral soil. | Xiang W, Wang G, Wang Y, Yao R, Zhang F, Wang R, Wang D, Zheng S | Int J Syst Evol Microbiol | 10.1099/ijs.0.063701-0 | 2014 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Selenium, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
6140 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15890) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15890 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
31407 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 27716 | ||
36815 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7191 | |||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | ||
68371 | Automatically annotated from API 50CH acid | ||||||
68382 | Automatically annotated from API zym | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
80803 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID138998.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
116369 | Curators of the CIP | Collection of Institut Pasteur (CIP 109447) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109447 |