Strain identifier

BacDive ID: 11555

Type strain: Yes

Species: Paenibacillus azoreducens

Strain Designation: CM1

Strain history: CIP <- 2001, NCIMB <- G. Mc Mullan, Univ. Ulster, UK, strain: CM1, Paenibacillus azoreducens

NCBI tax ID(s): 116718 (species)

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General

@ref: 5174

BacDive-ID: 11555

DSM-Number: 13822

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, motile, rod-shaped

description: Paenibacillus azoreducens CM1 is a facultative anaerobe, mesophilic, motile bacterium that was isolated from mixed bacterial culture originally from textile waste water.

NCBI tax id

  • NCBI tax id: 116718
  • Matching level: species

strain history

@refhistory
5174<- G. Mc Mullan; CM1 (Paenibacillus azoreductus)
116687CIP <- 2001, NCIMB <- G. Mc Mullan, Univ. Ulster, UK, strain: CM1, Paenibacillus azoreducens

doi: 10.13145/bacdive11555.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus azoreducens
  • full scientific name: Paenibacillus azoreducens Meehan et al. 2001

@ref: 5174

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus azoreducens

full scientific name: Paenibacillus azoreducens Meehan et al. 2001

strain designation: CM1

type strain: yes

Morphology

cell morphology

  • @ref: 116687
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 116687

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5174NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40700MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116687CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5174positivegrowth30mesophilic
40700positivegrowth30mesophilic
116687positivegrowth30-45
116687nogrowth10psychrophilic
116687nogrowth22psychrophilic
116687nogrowth55thermophilic

culture pH

  • @ref: 116687
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 116687
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
116687NaClpositivegrowth0-4 %
116687NaClnogrowth6 %
116687NaClnogrowth8 %
116687NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose+builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
116687citrate-carbon source16947
116687esculin+hydrolysis4853
116687hippurate+hydrolysis606565
116687nitrate+reduction17632
116687nitrite-reduction16301
116687nitrate+respiration17632

metabolite production

  • @ref: 116687
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11668715688acetoin-
11668717234glucose+

enzymes

@refvalueactivityec
116687oxidase+
116687beta-galactosidase+3.2.1.23
116687alcohol dehydrogenase-1.1.1.1
116687gelatinase+/-
116687amylase+
116687DNase+
116687caseinase-3.4.21.50
116687catalase+1.11.1.6
116687tween esterase+
116687gamma-glutamyltransferase-2.3.2.2
116687lecithinase-
116687lipase-
116687lysine decarboxylase-4.1.1.18
116687ornithine decarboxylase-4.1.1.17
116687urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116687--++-+------++--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116687+-+-++--+/-+++/-+----+--+/-+/-++++++++++--+++-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116687+++++-+++--+++++--++-++----------+-+-----------------------++-+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5174mixed bacterial culture originally from textile waste waterNorthern IrelandUnited KingdomGBREurope
116687Environment, WastewaterNorthern IrelandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Engineered#Waste#Industrial wastewater
#Host#Microbial#Bacteria

taxonmaps

  • @ref: 69479
  • File name: preview.99_44890.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_2482;97_25681;98_32571;99_44890&stattab=map
  • Last taxonomy: Paenibacillus azoreducens subclade
  • 16S sequence: AJ272249
  • Sequence Identity:
  • Total samples: 917
  • soil counts: 381
  • aquatic counts: 69
  • animal counts: 393
  • plant counts: 74

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51741Risk group (German classification)
1166871Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Paenibacillus sp. CM1 partial 16S rRNA gene
  • accession: AJ272249
  • length: 1500
  • database: ena
  • NCBI tax ID: 116718

External links

@ref: 5174

culture collection no.: DSM 13822, NCIMB 13761, CIP 107224

straininfo link

  • @ref: 80783
  • straininfo: 49530

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11594595Paenibacillus azoreducens sp. nov., a synthetic azo dye decolorizing bacterium from industrial wastewater.Meehan C, Bjourson AJ, McMullan GInt J Syst Evol Microbiol10.1099/00207713-51-5-16812001Azo Compounds/*metabolism, Bacillus/*classification/genetics/metabolism, Base Composition, Biodegradation, Environmental, Coloring Agents/metabolism, DNA, Ribosomal/analysis, Fatty Acids/analysis, *Industrial Waste, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water Microbiology, Water Purification/*methodsMetabolism
Phylogeny15879272Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum.Rivas R, Gutierrez C, Abril A, Mateos PF, Martinez-Molina E, Ventosa A, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.63513-02005Aerobiosis, Argentina, Base Composition, Carbohydrate Metabolism, Catalase/analysis, Cicer/*microbiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Esculin/metabolism, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Gram-Positive Endospore-Forming Rods/*classification/cytology/*isolation & purification/physiology, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Oxidoreductases/analysis, Phenazines, Phylogeny, Plant Roots/*microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial, beta-Glucosidase/analysisEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5174Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13822)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13822
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40700Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4636
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80783Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49530.1StrainInfo: A central database for resolving microbial strain identifiers
116687Curators of the CIPCollection of Institut Pasteur (CIP 107224)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107224