Strain identifier
BacDive ID: 11511
Type strain:
Species: Paenibacillus kobensis
Strain Designation: YK205
Strain history: CIP <- 1995, IFO <- 1955, T. Harada, Kobe Women's Univ., Japan: strain YK205
NCBI tax ID(s): 1220566 (strain), 59841 (species)
General
@ref: 3869
BacDive-ID: 11511
DSM-Number: 10249
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, motile
description: Paenibacillus kobensis YK205 is a spore-forming, mesophilic, motile bacterium that was isolated from soil.
NCBI tax id
NCBI tax id | Matching level |
---|---|
59841 | species |
1220566 | strain |
strain history
@ref | history |
---|---|
3869 | <- IFO |
67770 | NBRC 15729 <-- IFO 15729 <-- T. Harada YK205. |
120455 | CIP <- 1995, IFO <- 1955, T. Harada, Kobe Women's Univ., Japan: strain YK205 |
doi: 10.13145/bacdive11511.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Paenibacillaceae
- genus: Paenibacillus
- species: Paenibacillus kobensis
- full scientific name: Paenibacillus kobensis (Kanzawa et al. 1995) Shida et al. 1997
synonyms
- @ref: 20215
- synonym: Bacillus kobensis
@ref: 3869
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Paenibacillaceae
genus: Paenibacillus
species: Paenibacillus kobensis
full scientific name: Paenibacillus kobensis (Kanzawa et al. 1995) Shida et al. 1997
strain designation: YK205
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 95.851 | ||
69480 | 100 | positive | ||
120455 | yes | positive | rod-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3869 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92; with strain-specific modifications) Composition: Trypticase soy broth 30.0 g/l Yeast extract 3.0 g/l Distilled water |
41228 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
120455 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3869 | positive | growth | 30 | mesophilic |
41228 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
120455 | positive | growth | 30-37 | mesophilic |
120455 | no | growth | 10 | psychrophilic |
120455 | no | growth | 45 | thermophilic |
120455 | no | growth | 55 | thermophilic |
culture pH
- @ref: 120455
- ability: no
- type: growth
- pH: 6
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
120455 | yes |
compound production
- @ref: 3869
- compound: exo-(1-3)-ß-D glucanase
observation
- @ref: 67770
- observation: quinones: MK-7
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | + | builds acid from | 28087 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | + | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | - | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
120455 | citrate | - | carbon source | 16947 |
120455 | nitrate | + | reduction | 17632 |
120455 | nitrite | - | reduction | 16301 |
120455 | nitrate | - | respiration | 17632 |
metabolite production
- @ref: 120455
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 120455
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
120455 | oxidase | - | |
120455 | alcohol dehydrogenase | - | 1.1.1.1 |
120455 | gelatinase | - | |
120455 | catalase | + | 1.11.1.6 |
120455 | lysine decarboxylase | - | 4.1.1.18 |
120455 | ornithine decarboxylase | - | 4.1.1.17 |
120455 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120455 | - | - | + | + | - | - | - | - | - | - | + | + | - | - | - | - | + | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120455 | - | - | - | - | - | - | - | - | - | +/- | + | - | + | - | - | - | - | - | - | - | - | - | + | - | + | - | + | + | - | +/- | - | - | - | - | - | +/- | + | - | + | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120455 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
3869 | soil | Kobe City | Japan | JPN | Asia | |
67770 | Soil | Kobe City | Japan | JPN | Asia | |
120455 | Environment, Soil | Kobe | Japan | JPN | Asia | 1990 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_133374.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_2361;97_18918;98_33932;99_133374&stattab=map
- Last taxonomy: Paenibacillus kobensis subclade
- 16S sequence: AB073363
- Sequence Identity:
- Total samples: 3
- soil counts: 1
- plant counts: 2
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
3869 | 1 | Risk group (German classification) |
120455 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Paenibacillus kobensis gene for 16S rRNA, partial sequence | AB073363 | 1503 | ena | 59841 |
20218 | Paenibacillus kobensis DNA for 16S rRNA | D78471 | 1436 | ena | 59841 |
20218 | Paenibacillus kobensis gene for 16S rRNA, partial sequence, strain: NBRC 15729 | AB680952 | 1478 | ena | 59841 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Paenibacillus kobensis NBRC 15729 | 1220566.3 | wgs | patric | 1220566 |
67770 | Paenibacillus kobensis NBRC 15729 | GCA_004000825 | contig | ncbi | 1220566 |
GC content
- @ref: 3869
- GC-content: 51
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 85.407 | no |
gram-positive | yes | 85.352 | no |
anaerobic | no | 99.274 | no |
aerobic | yes | 89.897 | no |
halophile | no | 97.352 | no |
spore-forming | yes | 95.671 | no |
glucose-util | yes | 92.118 | no |
flagellated | yes | 78.2 | no |
thermophile | no | 99.006 | no |
glucose-ferment | no | 88.363 | no |
External links
@ref: 3869
culture collection no.: DSM 10249, IFO 15729, NBRC 15729, JCM 12164, ATCC 51900, CCM 4537, CIP 104576, HSCC 488, LMG 18049, NRRL B-23246
straininfo link
- @ref: 80743
- straininfo: 10547
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 8590679 | Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyze resistant curdlan. | Kanzawa Y, Harada A, Takeuchi M, Yokota A, Harada T | Int J Syst Bacteriol | 10.1099/00207713-45-3-515 | 1995 | Aerobiosis, Bacillus/*classification/genetics/metabolism, Bacterial Proteins/chemistry, Base Composition, Carbohydrate Metabolism, Culture Media, DNA, Bacterial/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Fatty Acids/chemistry, Glucans/*metabolism, Nucleic Acid Hybridization, Polysaccharides, Bacterial/*metabolism, Sequence Homology, Nucleic Acid, *Soil Microbiology, Spores, *beta-Glucans | Metabolism |
Phylogeny | 12361272 | Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune. | Dasman, Kajiyama S, Kawasaki H, Yagi M, Seki T, Fukusaki E, Kobayashi A | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1669 | 2002 | Bacillus/*classification/genetics/isolation & purification/metabolism, Base Composition, Base Sequence, Biodegradation, Environmental, Cyanobacteria/metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, Polysaccharides, Bacterial/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Soil Microbiology | Metabolism |
Phylogeny | 27188601 | Paenibacillus cisolokensis sp. nov., isolated from litter of a geyser. | Yokota A, Ningsih F, Nurlaili DG, Sakai Y, Yabe S, Oetari A, Santoso I, Sjamsuridzal W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001151 | 2016 | Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Indonesia, Nucleic Acid Hybridization, Paenibacillus/classification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3869 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10249) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10249 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
41228 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16499 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
80743 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10547.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120455 | Curators of the CIP | Collection of Institut Pasteur (CIP 104576) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104576 |