Strain identifier

BacDive ID: 11508

Type strain: Yes

Species: Paenibacillus popilliae

Strain history: CIP <- 1999, A. Yousten, Virginia Polytechnic Inst., Blacksburg, USA <- ATCC <- NRRL <- O. Lysenko and H. Tashiro

NCBI tax ID(s): 1212764 (strain), 78057 (species)

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General

@ref: 16471

BacDive-ID: 11508

DSM-Number: 22700

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic, motile, animal pathogen

description: Paenibacillus popilliae DSM 22700 is a microaerophile, spore-forming, mesophilic animal pathogen that was isolated from commercial spore dust.

NCBI tax id

NCBI tax idMatching level
78057species
1212764strain

strain history

@refhistory
16471<- CIP <- A. Yousten, VPI <- ATCC <- NRRL <- A. Lysenko and H. Tashiro
121940CIP <- 1999, A. Yousten, Virginia Polytechnic Inst., Blacksburg, USA <- ATCC <- NRRL <- O. Lysenko and H. Tashiro

doi: 10.13145/bacdive11508.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus popilliae
  • full scientific name: Paenibacillus popilliae (Dutky 1940) Pettersson et al. 1999

  • synonyms

    • @ref: 20215
    • synonym: Bacillus popilliae

@ref: 16471

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus popilliae

full scientific name: Paenibacillus popilliae (Dutky 1940) Pettersson et al. 1999

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.172
69480100positive
121940nopositiverod-shaped

colony morphology

  • @ref: 121940

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16471COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16471BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40281MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
121940CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
16471positivegrowth30mesophilic
40281positivegrowth30mesophilic
49440positivegrowth37mesophilic
121940positivegrowth22-30
121940nogrowth10psychrophilic
121940nogrowth37mesophilic
121940nogrowth45thermophilic
121940nogrowth55thermophilic

culture pH

  • @ref: 121940
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16471microaerophile
49440aerobe
121940facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100
121940yes

halophily

@refsaltgrowthtested relationconcentration
121940NaClpositivegrowth0 %
121940NaClnogrowth2 %
121940NaClnogrowth4 %
121940NaClnogrowth6 %
121940NaClnogrowth8 %
121940NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121940citrate-carbon source16947
121940esculin-hydrolysis4853
121940hippurate+hydrolysis606565
121940nitrate-reduction17632
121940nitrite-reduction16301
121940nitrate+respiration17632

metabolite production

  • @ref: 121940
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12194015688acetoin-
12194017234glucose-

enzymes

@refvalueactivityec
121940oxidase-
121940beta-galactosidase-3.2.1.23
121940alcohol dehydrogenase-1.1.1.1
121940gelatinase-
121940amylase-
121940DNase-
121940caseinase-3.4.21.50
121940catalase-1.11.1.6
121940tween esterase-
121940gamma-glutamyltransferase-2.3.2.2
121940lecithinase-
121940lipase-
121940lysine decarboxylase-4.1.1.18
121940ornithine decarboxylase-4.1.1.17
121940protease-
121940urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121940-+++------++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121940----------+/-+/-+--------+/-+/-++++/-+/----------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
16471commercial spore dustUSAUSANorth America
49440Commercial spore dust *)
121940Environment, Commercial spore dustMexicoMEXNorth AmericaGuanajuato

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Engineered product
#Engineered#Waste#Dust (Ash)
#Host#Microbial#Bacteria

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16471yes1Risk group (German classification)
1219401Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paenibacillus popilliae strain ATCC14706(T) 16S ribosomal RNA gene, partial sequenceAF0718591470ena1212764
20218Paenibacillus popilliae strain NRRL B-2309 16S ribosomal RNA gene, partial sequenceEF1904871464ena1212764
20218B.popilliae 16S ribosomal RNAX606331427ena1212764
16471Paenibacillus popilliae gene for 16S rRNA, partial sequenceAB0731981503ena1212764

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus popilliae ATCC 14706GCA_000315235contigncbi1212764
66792Paenibacillus popilliae ATCC 147061212764.3wgspatric1212764
66792Paenibacillus popilliae ATCC 147062537561634draftimg1212764

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes83.593no
gram-positiveyes80.846no
anaerobicno94.732no
aerobicyes64.067no
halophileno89.285no
spore-formingyes96.004no
thermophileno98.409yes
glucose-utilyes83.074no
flagellatedyes61.26no
glucose-fermentno80.368no

External links

@ref: 16471

culture collection no.: DSM 22700, ATCC 14706, CCUG 28881, CIP 106066, NCCB 75017, NRRL B-2309, KCTC 3806

straininfo link

  • @ref: 80740
  • straininfo: 10575

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny345972Characteristics of the constituent substrains of Bacillus popilliae growing in batch and continuous cultures.Sharpe ES, Bulla LA JrAppl Environ Microbiol10.1128/aem.35.3.601-609.19781978Bacillus/*growth & development, *Bacteriological Techniques, *Insect Control, *Pest Control, Biological, Species Specificity, Spores, Bacterial/growth & development
Cultivation5418948Characteristics of a new strain of Bacillus popilliae sporogenic in vitro.Sharpe ES, St Julian G, Crowell CAppl Microbiol10.1128/am.19.4.681-688.19701970Agar, Bacillus/*growth & development/pathogenicity, Coleoptera, Culture Media, Disaccharides/metabolism, Glycosides, Larva, Phosphates, Spores/*growth & development, YeastsMetabolism
Phylogeny10319474Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.Pettersson B, Rippere KE, Yousten AA, Priest FGInt J Syst Bacteriol10.1099/00207713-49-2-5311999Animals, Bacillus/*classification/*genetics/physiology, DNA Primers, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Insecta/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Genetics15644892Characterization of Paenibacillus popilliae rRNA operons.Dingman DWCan J Microbiol10.1139/w04-0682004Bacillus subtilis, Base Sequence, Blotting, Southern, Chromosomes, Bacterial, DNA Fingerprinting, DNA, Bacterial, DNA, Ribosomal/chemistry, Escherichia coli, Gene Order/genetics, Genes, rRNA, Gram-Positive Endospore-Forming Bacteria/*genetics, Molecular Sequence Data, Protein Biosynthesis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, RNA, Ribosomal, 5S/genetics, rRNA Operon/*geneticsPhylogeny
Metabolism15980329Putative VanRS-like two-component regulatory system associated with the inducible glycopeptide resistance cluster of Paenibacillus popilliae.Fraimow H, Knob C, Herrero IA, Patel RAntimicrob Agents Chemother10.1128/AAC.49.7.2625-2633.20052005Amino Acid Sequence, Animals, Anti-Bacterial Agents/*pharmacology, Bacteria/*drug effects/genetics/growth & development/metabolism, Bacterial Proteins/*genetics/*metabolism, Base Sequence, *Drug Resistance, Bacterial, *Gene Expression Regulation, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, *Multigene Family, Peptide Synthases/genetics/metabolism, Protein Kinases/genetics/*metabolism, Sequence Analysis, DNA, Signal Transduction, Transcription Factors/genetics/*metabolism, Vancomycin/pharmacology, Vancomycin ResistanceGenetics
Enzymology16535060Comparison of Pulsed-Field Gel Electrophoresis DNA Fingerprints of Field Isolates of the Entomopathogen Bacillus popilliae.Macdonald R, Kalmakoff JAppl Environ Microbiol10.1128/aem.61.6.2446-2449.19951995
25853059Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706(T).Iiyama K, Mon H, Mori K, Mitsudome T, Lee JM, Kusakabe T, Tashiro K, Asano S, Yasunaga-Aoki CMeta Gene10.1016/j.mgene.2015.03.0012015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16471Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22700)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22700
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40281Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18154
49440Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28881)https://www.ccug.se/strain?id=28881
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80740Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10575.1StrainInfo: A central database for resolving microbial strain identifiers
121940Curators of the CIPCollection of Institut Pasteur (CIP 106066)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106066