Strain identifier

BacDive ID: 1149

Type strain: Yes

Species: Heyndrickxia sporothermodurans

Strain Designation: M 215, MB581, MB 581

Strain history: CIP <- 1996, F.G. Priest, Heriot Watt Univ., Edinburgh, Scotland, UK: strain M 215 <- F. Lembke

NCBI tax ID(s): 46224 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3982

BacDive-ID: 1149

DSM-Number: 10599

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Heyndrickxia sporothermodurans M 215 is an aerobe, spore-forming, mesophilic bacterium that was isolated from UHT-milk.

NCBI tax id

  • NCBI tax id: 46224
  • Matching level: species

strain history

@refhistory
3982<- Lembke, Tetrapak GmbH
123274CIP <- 1996, F.G. Priest, Heriot Watt Univ., Edinburgh, Scotland, UK: strain M 215 <- F. Lembke

doi: 10.13145/bacdive1149.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Heyndrickxia
  • species: Heyndrickxia sporothermodurans
  • full scientific name: Heyndrickxia sporothermodurans (Pettersson et al. 1996) Gupta et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Bacillus sporothermodurans

@ref: 3982

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Heyndrickxia

species: Heyndrickxia sporothermodurans

full scientific name: Heyndrickxia sporothermodurans (Pettersson et al. 1996) Gupta et al. 2020

strain designation: M 215, MB581, MB 581

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
29921positiverod-shapedyes
69480yes96.598
69480positive100
123274positiverod-shapedno

pigmentation

  • @ref: 29921
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3982BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38662MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123274CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
3982positivegrowth30mesophilic
29921positivegrowth20-55
29921positiveoptimum37mesophilic
38662positivegrowth30mesophilic
123274positivegrowth22-45
123274nogrowth10psychrophilic
123274nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
29921positivegrowth05-09alkaliphile
123274positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29921aerobe
123274obligate aerobe

spore formation

@refspore formationconfidence
29921yes
69481yes100
69480yes100
123274yes

halophily

@refsaltgrowthtested relationconcentration
29921NaClpositivegrowth<5 %
123274NaClpositivegrowth0-4 %
123274NaClnogrowth6 %
123274NaClnogrowth8 %
123274NaClnogrowth10 %

observation

  • @ref: 29921
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2992117057cellobiose+carbon source
2992128757fructose+carbon source
2992117234glucose+carbon source
2992117754glycerol+carbon source
2992117306maltose+carbon source
2992129864mannitol+carbon source
2992137684mannose+carbon source
2992137657methyl D-glucoside+carbon source
2992117814salicin+carbon source
2992117992sucrose+carbon source
2992127082trehalose+carbon source
2992117151xylitol+carbon source
299214853esculin+hydrolysis
2992117632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
1232744853esculin+hydrolysis
123274606565hippurate+hydrolysis
12327417632nitrate-reduction
12327416301nitrite-reduction
12327417632nitrate-respiration

metabolite production

  • @ref: 123274
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12327415688acetoin-
12327417234glucose-

enzymes

@refvalueactivityec
29921catalase+1.11.1.6
29921gelatinase+
29921cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123274oxidase+
123274beta-galactosidase-3.2.1.23
123274alcohol dehydrogenase-1.1.1.1
123274gelatinase-
123274amylase-
123274DNase+
123274caseinase-3.4.21.50
123274catalase+1.11.1.6
123274tween esterase-
123274gamma-glutamyltransferase-2.3.2.2
123274lecithinase-
123274lipase-
123274lysine decarboxylase-4.1.1.18
123274ornithine decarboxylase-4.1.1.17
123274phenylalanine ammonia-lyase+4.3.1.24
123274protease-
123274urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123274-+++------++---+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123274+/----+/------++-----++/-----+++++--++---+/----+-+-------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
3982UHT-milkItalyITAEurope
123274Food, MilkItalyITAEurope1993

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_38671.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1672;97_2002;98_28353;99_38671&stattab=map
  • Last taxonomy: Bacillus sporothermodurans
  • 16S sequence: U49078
  • Sequence Identity:
  • Total samples: 721
  • soil counts: 172
  • aquatic counts: 163
  • animal counts: 322
  • plant counts: 64

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39821Risk group (German classification)
1232741Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus sporothermodurans strain DSM10599(T) rrnA operon 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF071850222ena46224
20218Bacillus sporothermodurans strain DSM10599(T) rrnB operon 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF071852386ena46224
3982Bacillus sporothermodurans 16S rRNA type I gene, partial sequenceU490781497ena46224

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Heyndrickxia sporothermodurans DSM 10599GCA_016785185contigncbi46224
66792Heyndrickxia sporothermodurans DSM 10599GCA_003055085scaffoldncbi46224
66792Heyndrickxia sporothermodurans DSM 10599GCA_016756695chromosomencbi46224
66792Bacillus sporothermodurans strain DSM 1059946224.153wgspatric46224
66792Bacillus sporothermodurans strain DSM 1059946224.121completepatric46224
66792Bacillus sporothermodurans strain DSM 1059946224.16wgspatric46224
66792Bacillus sporothermodurans strain L1_14046224.137wgspatric46224
66792Bacillus sporothermodurans strain L1_14146224.138wgspatric46224
66792Bacillus sporothermodurans strain L1_14246224.142wgspatric46224
66792Bacillus sporothermodurans strain L1_14346224.136wgspatric46224
66792Bacillus sporothermodurans strain L1_14446224.135wgspatric46224
66792Bacillus sporothermodurans strain L1_14546224.132wgspatric46224
66792Bacillus sporothermodurans strain L1_14646224.133wgspatric46224
66792Bacillus sporothermodurans strain L1_14946224.150wgspatric46224
66792Bacillus sporothermodurans strain L1_15046224.130wgspatric46224
66792Bacillus sporothermodurans strain L1_15146224.134wgspatric46224
66792Bacillus sporothermodurans strain L1_15246224.149wgspatric46224
66792Bacillus sporothermodurans strain L1_15346224.151wgspatric46224
66792Bacillus sporothermodurans strain L1_15446224.131wgspatric46224
66792Bacillus sporothermodurans strain L1_15546224.128wgspatric46224
66792Bacillus sporothermodurans strain L1_15646224.152wgspatric46224
66792Bacillus sporothermodurans strain L1_15746224.129wgspatric46224
66792Bacillus sporothermodurans strain L1_15846224.127wgspatric46224
66792Bacillus sporothermodurans strain L1_15946224.126wgspatric46224
66792Bacillus sporothermodurans strain L1_16046224.125wgspatric46224
66792Bacillus sporothermodurans strain L1_16146224.122wgspatric46224
66792Bacillus sporothermodurans strain M3420-1246224.145wgspatric46224
66792Bacillus sporothermodurans strain M5055-A46224.146wgspatric46224
66792Bacillus sporothermodurans strain M5233-646224.144wgspatric46224
66792Bacillus sporothermodurans strain M5311-146224.147wgspatric46224
66792Bacillus sporothermodurans strain M5311-246224.148wgspatric46224
66792Bacillus sporothermodurans strain M5352-10246224.124wgspatric46224
66792Bacillus sporothermodurans strain M5523-446224.143wgspatric46224
66792Bacillus sporothermodurans strain M5530-1746224.141wgspatric46224
66792Bacillus sporothermodurans strain M5547-2046224.139wgspatric46224
66792Bacillus sporothermodurans strain M5547-2546224.140wgspatric46224
66792Bacillus sporothermodurans strain M5572-646224.123wgspatric46224

GC content

@refGC-contentmethod
398236.7high performance liquid chromatography (HPLC)
2992136.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes87.832yes
anaerobicno98.937yes
halophileyes64.78no
spore-formingyes94.925no
glucose-utilyes85.543yes
aerobicyes93.544yes
flagellatedyes63.177no
thermophileno93.848no
motileyes85.408no
glucose-fermentno87.143no

External links

@ref: 3982

culture collection no.: DSM 10599, LMG 17668, CIP 104910, LMG 17894

straininfo link

  • @ref: 70820
  • straininfo: 10235

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11837291Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.).Reva ON, Smirnov VV, Pettersson B, Priest FGInt J Syst Evol Microbiol10.1099/00207713-52-1-1012002Bacillus/*classification/*genetics/isolation & purification/physiology, DNA, Bacterial/analysis/genetics, DNA, Ribosomal Spacer/analysis/genetics, Genes, rRNA/genetics, Gossypium/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Random Amplified Polymorphic DNA Technique, Ribotyping, Sequence Analysis, DNAGenetics
Phylogeny16166720Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin.Albert RA, Archambault J, Rossello-Mora R, Tindall BJ, Matheny MInt J Syst Evol Microbiol10.1099/ijs.0.02337-02005Bacillus/chemistry/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil, *Soil Microbiology, Sphagnopsida/*metabolism, *Water MicrobiologyGenetics
Stress20417981The effects of wet heat treatment on the structural and chemical components of Bacillus sporothermodurans spores.Tabit FT, Buys EInt J Food Microbiol10.1016/j.ijfoodmicro.2010.03.0332010Bacillus/*chemistry/growth & development, Bacterial Proteins/analysis, Hot Temperature, *Microbial Viability, Picolinic Acids/analysis, Spores, Bacterial/*chemistry/growth & development, Sterilization/*methods
Phylogeny27055557Bacillus vini sp. nov. isolated from alcohol fermentation pit mud.Ma K, Chen X, Guo X, Wang Y, Wang H, Zhou S, Song J, Kong D, Zhu J, Dong W, He M, Hu G, Zhao B, Ruan ZArch Microbiol10.1007/s00203-016-1218-42016Alcohols/*metabolism, *Bacillus/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/metabolism, China, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Fermentation, Glycolipids/analysis, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylethanolamines/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil MicrobiologyMetabolism
Phylogeny27497907Development of a real-time PCR assay for direct detection and quantification of Bacillus sporothermodurans in ultra-high temperature milk.Abouelnaga M, Lamas A, Miranda JM, Osman M, Cepeda A, Franco CMJ Dairy Sci10.3168/jds.2016-108522016Animals, Bacillus/*classification, Milk/*microbiology, RNA, Ribosomal, 16S/genetics, Real-Time Polymerase Chain Reaction, Temperature
27692715Evaluation of modified stainless steel surfaces targeted to reduce biofilm formation by common milk sporeformers.Jindal S, Anand S, Huang K, Goddard J, Metzger L, Amamcharla JJ Dairy Sci10.3168/jds.2016-113952016Animals, Bacterial Adhesion, *Biofilms, Milk, Polytetrafluoroethylene, *Stainless Steel
Genetics31147427Genome Sequences of Bacillus sporothermodurans Strains Isolated from Ultra-High-Temperature Milk.Owusu-Darko R, Allam M, de Oliveira SD, Ferreira CAS, Grover S, Mtshali S, Ismail A, Mallappa RH, Tabit F, Buys EMMicrobiol Resour Announc10.1128/MRA.00145-192019Phylogeny
Genetics33530338Taxonomic Evaluation of the Heyndrickxia (Basonym Bacillus) sporothermodurans Group (H. sporothermodurans, H. vini, H. oleronia) Based on Whole Genome Sequences.Fiedler G, Herbstmann AD, Doll E, Wenning M, Brinks E, Kabisch J, Breitenwieser F, Lappann M, Bohnlein C, Franz CMAPMicroorganisms10.3390/microorganisms90202462021
36337108Recovery potential of cavitation-induced injured cells of common spore-forming bacteria in skim milk exposed to ultrasonication.Almalki TA, Anand SJDS Commun10.3168/jdsc.2021-01092021

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
3982Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10599)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10599
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29921Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126292
38662Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16871
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70820Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10235.1StrainInfo: A central database for resolving microbial strain identifiers
123274Curators of the CIPCollection of Institut Pasteur (CIP 104910)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104910