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Strain identifier

BacDive ID: 11461

Type strain: Yes

Species: Paenibacillus alvei

Strain Designation: IMAB B-3-4, 662, Lochhead 127

Strain history: IAM 1258 <-- IFO 3343 <-- IMAB B-3-4 <-- ATCC 6344 <-- N. R. Smith NRS 662 <-- A. G. Lochhead 127.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2030

BacDive-ID: 11461

DSM-Number: 29

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen

description: Paenibacillus alvei IMAB B-3-4 is a mesophilic human pathogen that was isolated from foulbrood of bees.

NCBI tax id

NCBI tax idMatching level
44250species
1206781strain

strain history

doi: 10.13145/bacdive11461.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus alvei
  • full scientific name: Paenibacillus alvei (Cheshire and Cheyne 1885) Ash et al. 1994
  • synonyms

    • @ref: 20215
    • synonym: Bacillus alvei

@ref: 2030

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus alvei

full scientific name: Paenibacillus alvei (Cheshire and Cheyne 1885) Ash et al. 1994

strain designation: IMAB B-3-4, 662, Lochhead 127

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes78.409
6948084.666positive

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37614MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
2030NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1

culture temp

@refgrowthtypetemperaturerange
2030positivegrowth30mesophilic
37614positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 98.82

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 99.43

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose-fermentation
6837917306maltose+fermentation
6837916899D-mannitol-fermentation
6837965327D-xylose-fermentation
6837916988D-ribose+fermentation
683795291gelatin-hydrolysis
6837916199urea+hydrolysis
683794853esculin+hydrolysis
6837917632nitrate-reduction

enzymes

@refvalueactivityec
68379catalase-1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
44430--++-+++++--+--+-+--

Isolation, sampling and environmental information

isolation

@refsample type
2030foulbrood of bees
44430Foul brood of bees
67770Blood of bees

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Arthropoda#Insecta

taxonmaps

  • @ref: 69479
  • File name: preview.99_3682.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_1266;97_1501;98_2815;99_3682&stattab=map
  • Last taxonomy: Paenibacillus alvei subclade
  • 16S sequence: AJ320491
  • Sequence Identity:
  • Total samples: 2951
  • soil counts: 1858
  • aquatic counts: 305
  • animal counts: 422
  • plant counts: 366

Safety information

risk assessment

  • @ref: 2030
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paenibacillus alvei clone BALV-1R 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478098420ena1206781
20218Paenibacillus alvei 16S ribosomal RNA gene, partial sequenceAY862508815ena1206781
20218B.alvei 16S rRNAX573041552ena1206781
20218Paenibacillus alvei partial 16S rRNA gene, strain DSM 29TAJ3204911527ena1206781
20218Paenibacillus alvei gene for 16S rRNA, partial sequenceAB006944278ena1206781
20218Paenibacillus alvei gene for 16S rRNA, partial sequenceAB0732001501ena1206781
20218Paenibacillus alvei strain IFO3343 DNA for 16S ribosomal RNA, partial sequenceD783171433ena1206781
20218Paenibacillus alvei gene for 16S rRNA, partial sequence, strain: NBRC 3343AB6800691476ena44250
20218B.alvei 16S ribosomal RNAX606041434ena1206781

Genome sequences

  • @ref: 67770
  • description: Paenibacillus alvei DSM 29 DSM 29
  • accession: GCA_000293805
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1206781

GC content

@refGC-contentmethod
203046.2Buoyant density centrifugation (BD)
203044.6thermal denaturation, midpoint method (Tm)

External links

@ref: 2030

culture collection no.: DSM 29, ATCC 6344, CCM 2051, IFO 3343, LMG 13253, NBRC 3343, NCIB 9371, NCTC 6352, CCUG 1815, ATCC 8240, NCTC 3324, NRS 662, JCM 20131, CECT 2, CIP 66.18, CN 2198, IAM 1258, LMG 6922, NCCB 48008, NCFB 1153, NCIMB 8198, NCIMB 9371, NRRL B-383, VKM B-502

straininfo link

@refpassport
20218http://www.straininfo.net/strains/38950
20218http://www.straininfo.net/strains/38943
20218http://www.straininfo.net/strains/38945
20218http://www.straininfo.net/strains/38952
20218http://www.straininfo.net/strains/38953
20218http://www.straininfo.net/strains/38942
20218http://www.straininfo.net/strains/481211
20218http://www.straininfo.net/strains/122142
20218http://www.straininfo.net/strains/38961

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics1446307Artificial antigens. Synthetic carbohydrate haptens immobilized on crystalline bacterial surface layer glycoproteins.Messner P, Mazid MA, Unger FM, Sleytr UBCarbohydr Res10.1016/s0008-6215(00)90929-71992Animals, Antibody Formation/immunology, Antigens/*immunology, Bacillus/*chemistry, Carbohydrate Sequence, Clostridium/*chemistry, Cross-Linking Reagents, Glutaral, Haptens/*immunology, *Membrane Glycoproteins, Mice, Molecular Sequence Data, Molecular Structure, Oligosaccharides/*immunology
Enzymology2043344Structure of the glycan chain from the surface layer glycoprotein of Bacillus alvei CCM 2051.Altman E, Brisson JR, Messner P, Sleytr UBBiochem Cell Biol10.1139/o91-0101991Bacillus/*analysis/ultrastructure, Carbohydrate Conformation, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Freeze Etching, Glycoproteins/*analysis, Magnetic Resonance Spectroscopy, Methylation, Molecular Structure, Molecular Weight, Polysaccharides/*chemistry
Genetics7721708Similarity of "core" structures in two different glycans of tyrosine-linked eubacterial S-layer glycoproteins.Messner P, Christian R, Neuninger C, Schulz GJ Bacteriol10.1128/jb.177.8.2188-2193.19951995Bacillus/chemistry, Bacterial Outer Membrane Proteins/*chemistry, *Bacterial Proteins, Carbohydrate Conformation, Carbohydrate Sequence, Clostridium/chemistry, Magnetic Resonance Spectroscopy, *Membrane Glycoproteins, Molecular Sequence Data, Molecular Structure, Polysaccharides/*chemistry, Tyrosine/chemistry
Genetics11425188A pyrophosphate bridge links the pyruvate-containing secondary cell wall polymer of Paenibacillus alvei CCM 2051 to muramic acid.Schaffer C, Muller N, Mandal PK, Christian R, Zayni S, Messner PGlycoconj J10.1023/a:10110623028892000Bacillus/*chemistry/cytology, Carbohydrate Conformation, Carbohydrate Sequence, Cell Wall/chemistry, Diphosphates/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Muramic Acids/*chemistry, Peptidoglycan/*chemistry/isolation & purification, Pyruvates/*chemistryEnzymology
Enzymology19304819Construction of a gene knockout system for application in Paenibacillus alvei CCM 2051T, exemplified by the S-layer glycan biosynthesis initiation enzyme WsfP.Zarschler K, Janesch B, Zayni S, Schaffer C, Messner PAppl Environ Microbiol10.1128/AEM.00087-092009Bacterial Proteins/analysis/genetics, Carbohydrates/analysis, Electrophoresis, Polyacrylamide Gel, Gene Knockout Techniques/*methods, Genetic Complementation Test, Genetic Vectors, Glycosyltransferases/genetics, Gram-Positive Bacteria/chemistry/*genetics, Membrane Glycoproteins/*biosynthesis, Models, Biological, Models, Molecular, Promoter Regions, Genetic
Metabolism20200052Protein tyrosine O-glycosylation--a rather unexplored prokaryotic glycosylation system.Zarschler K, Janesch B, Pabst M, Altmann F, Messner P, Schaffer CGlycobiology10.1093/glycob/cwq0352010Bacillus/chemistry/*metabolism, Bacterial Proteins/chemistry/genetics/*metabolism, Glycosylation, Mutation, Polysaccharides/chemistry/genetics/*metabolism, Reverse Transcriptase Polymerase Chain Reaction, Tyrosine/analogs & derivatives/chemistry/*metabolism
Phylogeny20513375Cell surface display of chimeric glycoproteins via the S-layer of Paenibacillus alvei.Zarschler K, Janesch B, Kainz B, Ristl R, Messner P, Schaffer CCarbohydr Res10.1016/j.carres.2010.04.0102010Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics/isolation & purification/metabolism, Base Sequence, Carbohydrate Sequence, Cell Wall/metabolism, Genetic Loci, Glycoproteins/chemistry/*genetics/isolation & purification/metabolism, Glycosylation, Molecular Sequence Data, Open Reading Frames/genetics, Paenibacillus/*cytology/*genetics, Phylogeny, Protein Engineering/*methods, Recombinant Fusion Proteins/chemistry/*genetics/isolation & purification/metabolismMetabolism
Genetics23105091Genome sequence of Paenibacillus alvei DSM 29, a secondary invader during European foulbrood outbreaks.Djukic M, Becker D, Poehlein A, Voget S, Daniel RJ Bacteriol10.1128/JB.01698-122012Animals, Bees/*microbiology, Disease Outbreaks, Europe, *Genome, Bacterial, Molecular Sequence Data, Paenibacillus/*classification/*geneticsPhylogeny
Metabolism23204458Are the surface layer homology domains essential for cell surface display and glycosylation of the S-layer protein from Paenibacillus alvei CCM 2051T?Janesch B, Messner P, Schaffer CJ Bacteriol10.1128/JB.01487-122012Amino Acid Motifs, Amino Acid Sequence, Bacterial Proteins/*physiology, Cell Membrane/physiology, Cell Wall/metabolism, Gene Expression Regulation, Bacterial/physiology, Glycoproteins/genetics/metabolism, Glycosylation, Membrane Glycoproteins/genetics/*metabolism, Paenibacillus/*physiology, Plasmids/genetics, Protein Structure, Tertiary
Metabolism24058714The S-layer homology domain-containing protein SlhA from Paenibacillus alvei CCM 2051(T) is important for swarming and biofilm formation.Janesch B, Koerdt A, Messner P, Schaffer CPLoS One10.1371/journal.pone.00765662013Amino Acid Motifs, Bacterial Proteins/*chemistry/genetics/*metabolism, *Biofilms, Flagella/genetics/metabolism, Paenibacillus/*physiology, Protein Structure, Tertiary
25893145Description of a Putative Oligosaccharyl:S-Layer Protein Transferase from the Tyrosine O-Glycosylation System of Paenibacillus alvei CCM 2051(T).Ristl R, Janesch B, Anzengruber J, Forsthuber A, Blaha J, Messner P, Schaffer CAdv Microbiol10.4236/aim.2012.240692012
Metabolism26405108Flagellin glycosylation in Paenibacillus alvei CCM 2051T.Janesch B, Schirmeister F, Maresch D, Altmann F, Messner P, Kolarich D, Schaffer CGlycobiology10.1093/glycob/cwv0872015Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, Flagellin/*metabolism, Glycosylation, Glycosyltransferases/genetics/metabolism, Hexoses/metabolism, Molecular Sequence Data, Mutation, Paenibacillus/enzymology/genetics/*metabolism, *Protein Processing, Post-TranslationalGenetics
Phylogeny28555445Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater.Simon L, Skraban J, Kyrpides NC, Woyke T, Shapiro N, Cleenwerck I, Vandamme P, Whitman WB, Trcek JAntonie Van Leeuwenhoek10.1007/s10482-017-0891-x2017Base Composition, Carbohydrate Metabolism, Cell Wall/chemistry, Enzymes/metabolism, Fimbriae, Bacterial/genetics, Genome Size, Genome, Bacterial, Lakes/microbiology, Nucleic Acid Hybridization, Paenibacillus/chemistry/*classification/physiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Slovenia, Species Specificity, Sugars/metabolism, Waste Water/*microbiologyMetabolism
Pathogenicity29760411Paenialvin A-D, four peptide antibiotics produced by Paenibacillus alvei DSM 29.Meng J, Zhong Z, Qian PYJ Antibiot (Tokyo)10.1038/s41429-017-0001-32018Anti-Bacterial Agents/*metabolism/*pharmacology, Antineoplastic Agents/pharmacology, Bacillus subtilis/*drug effects, Cell Line, Tumor, Drug Resistance, Multiple, Bacterial, Escherichia coli/*drug effects, HeLa Cells, Humans, Mass Spectrometry, Methicillin-Resistant Staphylococcus aureus/*drug effects, Microbial Sensitivity Tests, Nuclear Magnetic Resonance, Biomolecular, Paenibacillus/*metabolism, Pseudomonas aeruginosa/*drug effects, Rhodobacteraceae/*drug effectsMetabolism
Metabolism34822536Antifungal Activity of Biocontrol Agents In Vitro and Potential Application to Reduce Mycotoxins (Aflatoxin B1 and Ochratoxin A).Illueca F, Vila-Donat P, Calpe J, Luz C, Meca G, Quiles JMToxins (Basel)10.3390/toxins131107522021Aflatoxin B1/*metabolism, Antifungal Agents/*pharmacology, Bacillus amyloliquefaciens/metabolism, Cell-Free System, Fungicides, Industrial/*pharmacology, In Vitro Techniques, Ochratoxins/*metabolism, Paenibacillus/metabolism, Pantoea/metabolism, *Pest Control, Biological, Poisons/*metabolismBiotechnology
36118843Inhibitory activities of propolis, nisin, melittin and essential oil compounds on Paenibacillus alvei and Bacillus subtilis.Sani AA, Pereira AFM, Furlanetto A, de Sousa DSM, Zapata TB, Rall VLM, Fernandes AJ Venom Anim Toxins Incl Trop Dis10.1590/1678-9199-JVATITD-2022-00252022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2030Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29)https://www.dsmz.de/collection/catalogue/details/culture/DSM-29
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37614Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10240
44430Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 1815)https://www.ccug.se/strain?id=1815
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)