Strain identifier

BacDive ID: 11443

Type strain: Yes

Species: Cohnella hongkongensis

Strain Designation: HKU 3

Strain history: CIP <- 2003, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU3, Paenibacillus hongkongensis

NCBI tax ID(s): 178337 (species)

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General

@ref: 7104

BacDive-ID: 11443

DSM-Number: 17642

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Cohnella hongkongensis HKU 3 is an aerobe, spore-forming, mesophilic bacterium that was isolated from human blood.

NCBI tax id

  • NCBI tax id: 178337
  • Matching level: species

strain history

@refhistory
7104<- P. Kämpfer <- CCUG
399612003, K.Y. Yuen, Hong Kong, Paenibacillus hongkongensis: strain HKU3
119958CIP <- 2003, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU3, Paenibacillus hongkongensis

doi: 10.13145/bacdive11443.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Cohnella
  • species: Cohnella hongkongensis
  • full scientific name: Cohnella hongkongensis Kämpfer et al. 2006

  • synonyms

    • @ref: 20215
    • synonym: Paenibacillus hongkongensis

@ref: 7104

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Cohnella

species: Cohnella hongkongensis

full scientific name: Cohnella hongkongensis Kämpfer et al. 2006

strain designation: HKU 3

type strain: yes

Morphology

cell morphology

  • @ref: 119958
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119958

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7104CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
39961MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119958CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
7104positivegrowth30mesophilic
39961positivegrowth37mesophilic
58612positivegrowth37mesophilic
119958positivegrowth30-45
119958nogrowth10psychrophilic
119958nogrowth22psychrophilic
119958nogrowth55thermophilic

culture pH

  • @ref: 119958
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58612aerobe
119958obligate aerobe

spore formation

  • @ref: 119958
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
119958NaClnogrowth0 %
119958NaClnogrowth2 %
119958NaClnogrowth4 %
119958NaClnogrowth6 %
119958NaClnogrowth8 %
119958NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371D-arabitol+builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol+builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371L-arabinose+builds acid from30849
68371D-arabinose+builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
119958citrate-carbon source16947
119958esculin+hydrolysis4853
119958hippurate+hydrolysis606565
119958nitrate+reduction17632
119958nitrite-reduction16301

metabolite production

  • @ref: 119958
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11995815688acetoin-
11995817234glucose+

enzymes

@refvalueactivityec
119958oxidase-
119958beta-galactosidase+3.2.1.23
119958alcohol dehydrogenase-1.1.1.1
119958gelatinase-
119958amylase-
119958DNase-
119958caseinase-3.4.21.50
119958catalase+1.11.1.6
119958tween esterase-
119958gamma-glutamyltransferase-2.3.2.2
119958lecithinase-
119958lipase-
119958lysine decarboxylase-4.1.1.18
119958ornithine decarboxylase-4.1.1.17
119958urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119958-+++------+--+-++--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119958+-+++/-+--+/-++++++--++-++--++++++++-+/-+--++++/---+++/-+/--+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119958+++++++++++++-----+++++--++-+--+-++++----+------------++------------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7104human bloodHongkongChinaCHNAsia
58612Human bloodAsia
119958Human, BloodHong KongChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_164532.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_9809;97_79917;98_108097;99_164532&stattab=map
  • Last taxonomy: Cohnella hongkongensis subclade
  • 16S sequence: AF433165
  • Sequence Identity:
  • Total samples: 15
  • soil counts: 10
  • aquatic counts: 2
  • animal counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71041Risk group (German classification)
1199581Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7104
  • description: Paenibacillus hongkongensis 16S ribosomal RNA, partial sequence
  • accession: AF433165
  • length: 1468
  • database: ena
  • NCBI tax ID: 178337

GC content

  • @ref: 7104
  • GC-content: 47.6

External links

@ref: 7104

culture collection no.: DSM 17642, CCUG 49571, CIP 107898, DSM 15572

straininfo link

  • @ref: 80681
  • straininfo: 92048

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16585694Cohnella thermotolerans gen. nov., sp. nov., and classification of 'Paenibacillus hongkongensis' as Cohnella hongkongensis sp. nov.Kampfer P, Rossello-Mora R, Falsen E, Busse HJ, Tindall BJInt J Syst Evol Microbiol10.1099/ijs.0.63985-02006Fatty Acids/analysis, Gram-Positive Endospore-Forming Bacteria/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Phylogeny18050908Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from ginseng cultivating soil.Yoon MH, Ten LN, Im WTJ Microbiol Biotechnol2007Base Composition, Fatty Acids/analysis, Gram-Positive Endospore-Forming Bacteria/*classification/isolation & purification/metabolism, Panax/growth & development/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Vitamin K 2/analysis, Xylans/*metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitle
7104Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17642)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17642
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39961Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5459
58612Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 49571)https://www.ccug.se/strain?id=49571
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80681Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92048.1StrainInfo: A central database for resolving microbial strain identifiers
119958Curators of the CIPCollection of Institut Pasteur (CIP 107898)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107898