Strain identifier

BacDive ID: 11442

Type strain: Yes

Species: Cohnella thermotolerans

Strain history: CIP <- 2004, CCUG <- S. Karlstad, Stora Enso, Makhoul L

NCBI tax ID(s): 1121347 (strain), 329858 (species)

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General

@ref: 7125

BacDive-ID: 11442

DSM-Number: 17683

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Cohnella thermotolerans DSM 17683 is an aerobe, spore-forming, mesophilic bacterium that was isolated from sample of industrial starch production.

NCBI tax id

NCBI tax idMatching level
329858species
1121347strain

strain history

@refhistory
7125<- P. Kämpfer
123517CIP <- 2004, CCUG <- S. Karlstad, Stora Enso, Makhoul L

doi: 10.13145/bacdive11442.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Cohnella
  • species: Cohnella thermotolerans
  • full scientific name: Cohnella thermotolerans Kämpfer et al. 2006

@ref: 7125

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Cohnella

species: Cohnella thermotolerans

full scientific name: Cohnella thermotolerans Kämpfer et al. 2006

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31660positiverod-shapedno
69480yes96.955
69480positive100
123517positivecoccus-shaped

colony morphology

  • @ref: 123517

pigmentation

  • @ref: 31660
  • production: yes

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_17683_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17683_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17683_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17683_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_17683_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7125CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
36517MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123517CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123517CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
7125positivegrowth30mesophilic
31660positivegrowth25-55
31660positiveoptimum25-30mesophilic
36517positivegrowth37mesophilic
57600positivegrowth37mesophilic
123517positivegrowth30-55
123517nogrowth10psychrophilic
123517nogrowth22psychrophilic

culture pH

@refabilitytypepH
31660positivegrowth7
31660positiveoptimum7
123517positivegrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 31660
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
31660yes
69481yes100
69480yes100
123517yes

halophily

@refsaltgrowthtested relationconcentration
123517NaClpositivegrowth0 %
123517NaClnogrowth2 %
123517NaClnogrowth4 %
123517NaClnogrowth6 %
123517NaClnogrowth8 %
123517NaClnogrowth10 %

observation

  • @ref: 31660
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3166022599arabinose+carbon source
3166017057cellobiose+carbon source
3166028757fructose+carbon source
3166028260galactose+carbon source
3166024265gluconate+carbon source
3166017234glucose+carbon source
3166017306maltose+carbon source
3166037684mannose+carbon source
3166028053melibiose+carbon source
3166026546rhamnose+carbon source
3166033942ribose+carbon source
316604853esculin+hydrolysis
12351716947citrate-carbon source
1235174853esculin+hydrolysis
123517606565hippurate+hydrolysis
12351717632nitrate-reduction
12351716301nitrite-reduction
12351717632nitrate-respiration

metabolite production

  • @ref: 123517
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12351715688acetoin-
12351717234glucose-

enzymes

@refvalueactivityec
31660cytochrome oxidase+1.9.3.1
123517oxidase-
123517beta-galactosidase+3.2.1.23
123517alcohol dehydrogenase-1.1.1.1
123517gelatinase-
123517amylase+
123517DNase-
123517caseinase-3.4.21.50
123517catalase+1.11.1.6
123517tween esterase-
123517gamma-glutamyltransferase+2.3.2.2
123517lecithinase-
123517lipase-
123517lysine decarboxylase-4.1.1.18
123517ornithine decarboxylase-4.1.1.17
123517protease-
123517tryptophan deaminase-
123517urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123517--+--+----++++-+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123517+++++-+++++++++++++++++++-+-+--++++++--+-+-------------+++--+-------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
7125sample of industrial starch productionSwedenSWEEurope
57600Industrial starchSwedenSWEEurope2002-12-01
123517Environment, Industrial starchSwedenSWEEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Engineered#Industrial

taxonmaps

  • @ref: 69479
  • File name: preview.99_76659.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_13584;97_16545;98_20532;99_76659&stattab=map
  • Last taxonomy: Cohnella thermotolerans subclade
  • 16S sequence: AJ971483
  • Sequence Identity:
  • Total samples: 563
  • soil counts: 248
  • aquatic counts: 49
  • animal counts: 119
  • plant counts: 147

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71251Risk group (German classification)
1235171Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7125
  • description: Cohnia thermotolerans partial 16S rRNA gene, type strain CCUG 47242T
  • accession: AJ971483
  • length: 1486
  • database: ena
  • NCBI tax ID: 329858

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cohnella thermotolerans DSM 17683GCA_000429825scaffoldncbi1121347
66792Cohnella thermotolerans DSM 176831121347.4wgspatric1121347
66792Cohnella thermotolerans DSM 176832524023169draftimg1121347

GC content

  • @ref: 7125
  • GC-content: 59

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes84.192yes
flagellatedyes79.253no
gram-positiveyes87.614yes
anaerobicno98.625yes
aerobicyes89.512yes
halophileno96.66no
spore-formingyes95.909yes
thermophileno93.571no
glucose-utilyes87.889yes
glucose-fermentno91.554no

External links

@ref: 7125

culture collection no.: DSM 17683, CCUG 47242, CIP 108492

straininfo link

  • @ref: 80680
  • straininfo: 110965

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16585694Cohnella thermotolerans gen. nov., sp. nov., and classification of 'Paenibacillus hongkongensis' as Cohnella hongkongensis sp. nov.Kampfer P, Rossello-Mora R, Falsen E, Busse HJ, Tindall BJInt J Syst Evol Microbiol10.1099/ijs.0.63985-02006Fatty Acids/analysis, Gram-Positive Endospore-Forming Bacteria/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Phylogeny18050908Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from ginseng cultivating soil.Yoon MH, Ten LN, Im WTJ Microbiol Biotechnol2007Base Composition, Fatty Acids/analysis, Gram-Positive Endospore-Forming Bacteria/*classification/isolation & purification/metabolism, Panax/growth & development/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Vitamin K 2/analysis, Xylans/*metabolismMetabolism
Phylogeny19654349Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils.Kim SJ, Weon HY, Kim YS, Anandham R, Jeon YA, Hong SB, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.013581-02009Bacillales/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Panax/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, WetlandsMetabolism
Phylogeny19915111Cohnella thailandensis sp. nov., a xylanolytic bacterium from Thai soil.Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC, Lee JSInt J Syst Evol Microbiol10.1099/ijs.0.015859-02009Anaerobiosis, Bacillales/*classification/genetics/isolation & purification/*metabolism, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology, Thailand, Vitamin K 2/analysis, Xylans/*metabolismMetabolism
Phylogeny20097800Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil.Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC, Lee JSInt J Syst Evol Microbiol10.1099/ijs.0.017855-02010Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Xylans/*metabolismGenetics
Phylogeny23315409Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.Hameed A, Hung MH, Lin SY, Hsu YH, Liu YC, Shahina M, Lai WA, Huang HC, Young LS, Young CCInt J Syst Evol Microbiol10.1099/ijs.0.045831-02013Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Medicago sativa/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Taiwan, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny24994779Cohnella nanjingensis sp. nov., an extracellular polysaccharide-producing bacterium isolated from soil.Huang Z, Yu YJ, Bao YY, Xia L, Sheng XF, He LYInt J Syst Evol Microbiol10.1099/ijs.0.066456-02014Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Polysaccharides, Bacterial/biosynthesis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny34161218Cohnella terricola sp. nov., isolated from soil.Kim J, Chhetri G, Kim I, Kang M, Seo TInt J Syst Evol Microbiol10.1099/ijsem.0.0048352021Bacillales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
7125Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17683)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17683
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31660Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2794128776041
36517Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6224
57600Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47242)https://www.ccug.se/strain?id=47242
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80680Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID110965.1StrainInfo: A central database for resolving microbial strain identifiers
123517Curators of the CIPCollection of Institut Pasteur (CIP 108492)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108492