Strain identifier
BacDive ID: 1142
Type strain:
Species: Sutcliffiella horikoshii
Strain Designation: PN 121, JP 277
Strain history: CIP <- 1997, DSMZ <- H. Outtrup, Novo Nordisc A/S: strain PN 121
NCBI tax ID(s): 1076414 (strain), 79883 (species)
General
@ref: 3249
BacDive-ID: 1142
DSM-Number: 8719
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, motile, rod-shaped
description: Sutcliffiella horikoshii PN 121 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Bacillaceae.
NCBI tax id
NCBI tax id | Matching level |
---|---|
79883 | species |
1076414 | strain |
strain history
@ref | history |
---|---|
3249 | <- H. Outtrup, Novo Nordisk A/S; JP 277 |
119967 | CIP <- 1997, DSMZ <- H. Outtrup, Novo Nordisc A/S: strain PN 121 |
doi: 10.13145/bacdive1142.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Sutcliffiella
- species: Sutcliffiella horikoshii
- full scientific name: Sutcliffiella horikoshii (Nielsen et al. 1995) Gupta et al. 2020
synonyms
- @ref: 20215
- synonym: Bacillus horikoshii
@ref: 3249
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Sutcliffiella
species: Sutcliffiella horikoshii
full scientific name: Sutcliffiella horikoshii (Nielsen et al. 1995) Gupta et al. 2020
strain designation: PN 121, JP 277
type strain: yes
Morphology
cell morphology
- @ref: 119967
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3249 | ALKALINE NUTRIENT AGAR (DSMZ Medium 31) | yes | https://mediadive.dsmz.de/medium/31 | Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31; with strain-specific modifications) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaCl 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water |
35805 | MEDIUM 113 - for Bacillus | yes | Distilled water make up to (900.000 ml);Columbia agar (39.000 g);Solution 1 - M00343 (100.000 ml) | |
119967 | CIP Medium 113 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=113 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3249 | positive | growth | 30 | mesophilic |
35805 | positive | growth | 30 | mesophilic |
119967 | positive | growth | 10-41 | |
119967 | no | growth | 45 | thermophilic |
119967 | no | growth | 55 | thermophilic |
culture pH
- @ref: 119967
- ability: no
- type: growth
- pH: 6
Physiology and metabolism
oxygen tolerance
- @ref: 119967
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
119967 | NaCl | positive | growth | 0-8 % |
119967 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | + | builds acid from | 28087 |
68371 | starch | + | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
119967 | esculin | + | hydrolysis | 4853 |
119967 | hippurate | + | hydrolysis | 606565 |
119967 | nitrate | - | reduction | 17632 |
119967 | nitrite | - | reduction | 16301 |
119967 | nitrate | - | respiration | 17632 |
metabolite production
- @ref: 119967
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
119967 | 15688 | acetoin | - | |
119967 | 17234 | glucose | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
119967 | oxidase | - | |
119967 | beta-galactosidase | + | 3.2.1.23 |
119967 | alcohol dehydrogenase | - | 1.1.1.1 |
119967 | gelatinase | + | |
119967 | amylase | - | |
119967 | DNase | - | |
119967 | caseinase | - | 3.4.21.50 |
119967 | catalase | + | 1.11.1.6 |
119967 | tween esterase | - | |
119967 | gamma-glutamyltransferase | - | 2.3.2.2 |
119967 | lysine decarboxylase | - | 4.1.1.18 |
119967 | ornithine decarboxylase | - | 4.1.1.17 |
119967 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119967 | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119967 | - | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | + | +/- | + | + | + | +/- | + | - | - | - | + | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119967 | + | + | - | - | - | - | - | + | - | - | - | - | - | - | - | + | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - | + | - | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - | + | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
taxonmaps
- @ref: 69479
- File name: preview.99_977.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_567;97_659;98_762;99_977&stattab=map
- Last taxonomy: Bacillus horikoshii
- 16S sequence: X76443
- Sequence Identity:
- Total samples: 3305
- soil counts: 1032
- aquatic counts: 557
- animal counts: 523
- plant counts: 1193
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
3249 | 1 | Risk group (German classification) |
119967 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Bacillus horikoshii gene for 16S rRNA | AB043865 | 1521 | ena | 1076414 |
20218 | Bacillus horikoshii DSM 8719, 16S rRNA gene | X76443 | 1529 | ena | 1076414 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Sutcliffiella horikoshii DSM 8719 | GCA_001648575 | contig | ncbi | 1076414 |
66792 | Bacillus horikoshii DSM 8719 | 1076414.5 | wgs | patric | 1076414 |
66792 | Bacillus horikoshii DSM 8719 | 2731957965 | draft | img | 1076414 |
GC content
- @ref: 3249
- GC-content: 41.3
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 89.654 | no |
flagellated | yes | 86.021 | no |
gram-positive | yes | 87.803 | no |
anaerobic | no | 98.736 | no |
aerobic | yes | 92.301 | no |
halophile | yes | 64.275 | no |
spore-forming | yes | 95.203 | no |
thermophile | no | 96.28 | yes |
glucose-util | yes | 87.589 | no |
glucose-ferment | no | 94.017 | no |
External links
@ref: 3249
culture collection no.: DSM 8719, ATCC 700161, CIP 105300
straininfo link
- @ref: 70813
- straininfo: 10536
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24614848 | Bacillus tianshenii sp. nov., isolated from a marine sediment sample. | Jiang Z, Zhang DF, Khieu TN, Son CK, Zhang XM, Cheng J, Tian XP, Zhang S, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.062224-0 | 2014 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oceans and Seas, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Metabolism | 27084709 | Bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern. | Zhou YX, Liu GH, Liu B, Chen GJ, Du ZJ | Antonie Van Leeuwenhoek | 10.1007/s10482-016-0692-7 | 2016 | Alginates/*metabolism, Bacillus/genetics/*isolation & purification/*metabolism, Cell Wall/metabolism, China, Fatty Acids/metabolism, Genotype, Glucuronic Acid/metabolism, Hexuronic Acids/metabolism, Nucleic Acid Hybridization, Peptidoglycan/metabolism, Phenotype, Phosphatidylethanolamines/metabolism, Phylogeny, Sequence Analysis, DNA, Sodium Chloride, Soil/chemistry, *Soil Microbiology, Vitamin K 2/metabolism | Phylogeny |
Genetics | 27417833 | Draft Whole-Genome Sequence of the Type Strain Bacillus horikoshii DSM 8719. | Hernandez-Gonzalez IL, Olmedo-Alvarez G | Genome Announc | 10.1128/genomeA.00641-16 | 2016 | ||
Phylogeny | 35195511 | Sutcliffiella deserti sp. nov., isolated from desert soil. | Bai P, Zhang S, Chen Y, Ping W, Pang H, Du J, Li Y, Zhang J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005259 | 2022 | *Bacillaceae/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Mongolia, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3249 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8719) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8719 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
35805 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17304 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
70813 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10536.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119967 | Curators of the CIP | Collection of Institut Pasteur (CIP 105300) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105300 |