Strain identifier

BacDive ID: 11398

Type strain: Yes

Species: Aneurinibacillus thermoaerophilus

Strain Designation: L 420-91, L420-91

Strain history: CIP <- 1996, P. Messner, Bodenkultur Univ., Vienna, Austria: strain L420-91 <- F. Hollaus, Zuckerfabrik Leopoldsdorf, Austria

NCBI tax ID(s): 143495 (species)

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General

@ref: 3825

BacDive-ID: 11398

DSM-Number: 10154

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped

description: Aneurinibacillus thermoaerophilus L 420-91 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from sugar beet extraction juice.

NCBI tax id

  • NCBI tax id: 143495
  • Matching level: species

strain history

@refhistory
3825<- P. Messner, Univ. of Agricultural Sciences, Vienna <- F. Hollaus, Zuckerforschung Tulln <- Österr. Zuckerforschungsinst., Fuchsenbigl; L 420-91
123501CIP <- 1996, P. Messner, Bodenkultur Univ., Vienna, Austria: strain L420-91 <- F. Hollaus, Zuckerfabrik Leopoldsdorf, Austria

doi: 10.13145/bacdive11398.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Aneurinibacillus
  • species: Aneurinibacillus thermoaerophilus
  • full scientific name: Aneurinibacillus thermoaerophilus (Meier-Stauffer et al. 1996) Heyndrickx et al. 1997

  • synonyms

    • @ref: 20215
    • synonym: Bacillus thermoaerophilus

@ref: 3825

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Aneurinibacillus

species: Aneurinibacillus thermoaerophilus

full scientific name: Aneurinibacillus thermoaerophilus (Meier-Stauffer et al. 1996) Heyndrickx et al. 1997

strain designation: L 420-91, L420-91

type strain: yes

Morphology

cell morphology

  • @ref: 123501
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 123501

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3825NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39107MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123501CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
3825positivegrowth55thermophilic
39107positivegrowth55thermophilic
123501positivegrowth45-55thermophilic
123501nogrowth10psychrophilic
123501nogrowth22psychrophilic
123501nogrowth30mesophilic
123501nogrowth37mesophilic

culture pH

  • @ref: 123501
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 123501
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 123501
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
123501NaClpositivegrowth0-2 %
123501NaClnogrowth4 %
123501NaClnogrowth6 %
123501NaClnogrowth8 %
123501NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
123501citrate-carbon source16947
123501esculin-hydrolysis4853
123501hippurate-hydrolysis606565
123501nitrate-reduction17632
123501nitrite-reduction16301
123501nitrate-respiration17632

metabolite production

  • @ref: 123501
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12350115688acetoin-
12350117234glucose-

enzymes

@refvalueactivityec
123501oxidase+
123501beta-galactosidase-3.2.1.23
123501alcohol dehydrogenase-1.1.1.1
123501gelatinase+
123501amylase-
123501DNase+
123501caseinase+3.4.21.50
123501catalase-1.11.1.6
123501tween esterase+
123501gamma-glutamyltransferase-2.3.2.2
123501lecithinase-
123501lipase-
123501lysine decarboxylase-4.1.1.18
123501ornithine decarboxylase-4.1.1.17
123501protease-
123501tryptophan deaminase-
123501urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123501-+++-+----+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123501+/----+/------+/-----------+/------------------+/----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123501-------------------+-----------+-----------------+-----------+----------+--+--+-+--++---++++++--+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3825sugar beet extraction juiceLeopoldsdorfAustriaAUTEurope
123501Sugar beet extraction juiceLeopoldsdorfAustriaAUTEurope1991

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)
#Host Body Product#Plant#Juice (natural)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1034.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_162;96_593;97_694;98_809;99_1034&stattab=map
  • Last taxonomy: Aneurinibacillus thermoaerophilus subclade
  • 16S sequence: X94196
  • Sequence Identity:
  • Total samples: 3392
  • soil counts: 959
  • aquatic counts: 459
  • animal counts: 1694
  • plant counts: 280

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
38251Risk group (German classification)
1235011Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aneurinibacillus thermoaerophilus gene for 16S rRNA, partial sequence, strain:DSM 10154TAB1127261491ena143495
20218Aneurinibacillus thermoaerophilus isolate Ath2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723618952ena143495
20218Aneurinibacillus thermoaerophilus isolate Ath6 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU7236191172ena143495
20218Aneurinibacillus thermoaerophilus isolate Ath11 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU7236201088ena143495
20218B.thermoaerophilus 16S ribosomal RNA (strain L420-91, DSM 10154)X941961485ena143495

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aneurinibacillus thermoaerophilus strain L 420-91143495.3wgspatric143495
66792Aneurinibacillus thermoaerophilus DSM 101542634166356draftimg143495

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.575no
gram-positiveyes52.273no
anaerobicno96.308no
halophileno93.916no
spore-formingyes94.633no
thermophileyes86.531no
glucose-utilyes90.316no
aerobicyes77.99no
flagellatedyes55.761no
glucose-fermentno92.021no

External links

@ref: 3825

culture collection no.: DSM 10154, CIP 104816, LMG 17165

straininfo link

  • @ref: 80628
  • straininfo: 10039

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism12740380Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T.Pfoestl A, Hofinger A, Kosma P, Messner PJ Biol Chem10.1074/jbc.M3008582002003Acetylgalactosamine/analogs & derivatives/*biosynthesis, Amino Acid Sequence, Bacillaceae/enzymology/genetics/*metabolism, Base Sequence, Carbohydrate Epimerases/genetics/metabolism, DNA, Bacterial/genetics, Genes, Bacterial, Hydro-Lyases/genetics/metabolism, Molecular Sequence Data, Multigene Family, Nuclear Magnetic Resonance, Biomolecular, Nucleotidyltransferases/genetics/metabolism, Recombinant Proteins/genetics/metabolism, Restriction Mapping, Sequence Homology, Amino Acid, Thymine Nucleotides/*biosynthesisGenetics
Metabolism15012820An Aneurinibacillus sp. strain AM-1 produces a proline-specific aminopeptidase useful for collagen degradation.Murai A, Tsujimoto Y, Matsui H, Watanabe KJ Appl Microbiol10.1111/j.1365-2672.2004.02210.x2004Aminopeptidases/analysis/isolation & purification/*metabolism, Bacillaceae/*metabolism, Blotting, Western/methods, Collagen/*metabolism, Proline/metabolism, Sequence Analysis, Protein, Water PurificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3825Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10154)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10154
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39107Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16766
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80628Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10039.1StrainInfo: A central database for resolving microbial strain identifiers
123501Curators of the CIPCollection of Institut Pasteur (CIP 104816)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104816