Strain identifier
BacDive ID: 11380
Type strain:
Species: Bacillus aneurinilyticus
Strain Designation: Murayama
Strain history: CIP <- 1994, O. Shida, Higeta Shoyu Lab., Chiba, Japan <- Y. Ito <- R. Kimura
NCBI tax ID(s): 649747 (strain), 1391 (species)
General
@ref: 2190
BacDive-ID: 11380
DSM-Number: 5562
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, motile
description: Bacillus aneurinilyticus Murayama is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.
NCBI tax id
NCBI tax id | Matching level |
---|---|
649747 | strain |
1391 | species |
strain history
@ref | history |
---|---|
2190 | <- NRRL; NRS 1589 <- H. Iizuka, Univ. of Tokyo, 1970; strain Murayama |
67770 | O. Shida <-- ATCC 12856 <-- Y. Ito <-- R. Kimura. |
116380 | CIP <- 1994, O. Shida, Higeta Shoyu Lab., Chiba, Japan <- Y. Ito <- R. Kimura |
doi: 10.13145/bacdive11380.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus aneurinilyticus
- full scientific name: Bacillus aneurinilyticus corrig. (ex Kimura and Aoyama 1952) Shida et al. 1994
synonyms
@ref synonym 20215 Bacillus aneurinolyticus 20215 Aneurinibacillus aneurinolyticus 20215 Aneurinibacillus aneurinilyticus
@ref: 2190
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Paenibacillaceae
genus: Aneurinibacillus
species: Aneurinibacillus aneurinilyticus
full scientific name: Aneurinibacillus aneurinilyticus (Shida et al. 1994) Shida et al. 1996 emend. Heyndrickx et al. 1997
strain designation: Murayama
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 92.688 | ||
69480 | 100 | positive | ||
116380 | yes | positive | rod-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2190 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
38159 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
116380 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2190 | positive | growth | 30 | mesophilic |
38159 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
116380 | positive | growth | 30-55 | |
116380 | no | growth | 10 | psychrophilic |
culture pH
- @ref: 116380
- ability: positive
- type: growth
- pH: 6
Physiology and metabolism
oxygen tolerance
- @ref: 116380
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
116380 | yes |
compound production
- @ref: 67770
- compound: Thiamine hydrolase
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
116380 | NaCl | positive | growth | 0-4 % |
116380 | NaCl | no | growth | 6 % |
116380 | NaCl | no | growth | 8 % |
116380 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: MK-7
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | - | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | - | builds acid from | 16024 |
68371 | D-fructose | - | builds acid from | 15824 |
68371 | D-glucose | - | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
116380 | citrate | - | carbon source | 16947 |
116380 | nitrate | + | reduction | 17632 |
116380 | nitrite | - | reduction | 16301 |
116380 | nitrate | - | respiration | 17632 |
metabolite production
- @ref: 116380
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 116380
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
116380 | oxidase | + | |
116380 | alcohol dehydrogenase | - | 1.1.1.1 |
116380 | gelatinase | - | |
116380 | amylase | - | |
116380 | DNase | + | |
116380 | caseinase | - | 3.4.21.50 |
116380 | catalase | - | 1.11.1.6 |
116380 | lecithinase | + | |
116380 | lysine decarboxylase | - | 4.1.1.18 |
116380 | ornithine decarboxylase | - | 4.1.1.17 |
116380 | urease | - | 3.5.1.5 |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116380 | +/- | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | country | origin.country | continent | sample type |
---|---|---|---|---|
38159 | Japan | JPN | Asia | |
67770 | Human feces | |||
116380 | Japan | JPN | Asia | Human, Feces |
taxonmaps
- @ref: 69479
- File name: preview.99_916.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_162;96_542;97_625;98_723;99_916&stattab=map
- Last taxonomy: Aneurinibacillus
- 16S sequence: X94194
- Sequence Identity:
- Total samples: 1947
- soil counts: 881
- aquatic counts: 218
- animal counts: 736
- plant counts: 112
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2190 | 1 | Risk group (German classification) |
116380 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Aneurinibacillus aneurinolyticus DNA for 16S ribosomal RNA | D78455 | 1422 | ena | 649747 |
20218 | Aneurinibacillus aneurinilyticus gene for 16S rRNA, partial sequence | AB101592 | 1489 | ena | 649747 |
20218 | Aneurinibacillus aneurinilyticus gene for 16S rRNA, partial sequence, strain:DSM 5562T | AB112724 | 1489 | ena | 649747 |
20218 | Aneurinibacillus aneurinilyticus isolate Aa15 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723616 | 545 | ena | 649747 |
20218 | B.aneurinolyticus 16S ribosomal RNA | X94194 | 1482 | ena | 649747 |
20218 | Aneurinibacillus aneurinilyticus gene for 16S rRNA, partial sequence | AB271755 | 1464 | ena | 649747 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Aneurinibacillus aneurinilyticus ATCC 12856 | 649747.3 | wgs | patric | 649747 |
66792 | Aneurinibacillus aneurinilyticus ATCC 12856 | 2563366762 | draft | img | 649747 |
67770 | Aneurinibacillus aneurinilyticus ATCC 12856 | GCA_000466385 | scaffold | ncbi | 649747 |
GC content
- @ref: 67770
- GC-content: 42.9
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
gram-positive | yes | 69.006 | no |
anaerobic | no | 96.069 | no |
halophile | no | 93.066 | no |
spore-forming | yes | 94.517 | no |
glucose-util | yes | 85.93 | no |
thermophile | no | 98.486 | yes |
flagellated | yes | 55.089 | no |
aerobic | yes | 81.369 | no |
motile | yes | 87.17 | no |
glucose-ferment | no | 92.231 | no |
External links
@ref: 2190
culture collection no.: DSM 5562, ATCC 12856, CIP 104007, IAM 1077, IFO 15521, JCM 9024, NBRC 15521, NRS 1589, LMG 15531, LMG 16001, NRRL NRS-1589
straininfo link
- @ref: 80610
- straininfo: 10735
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2190 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5562) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5562 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
38159 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15868 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
80610 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID10735.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116380 | Curators of the CIP | Collection of Institut Pasteur (CIP 104007) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104007 |