Strain identifier

BacDive ID: 11339

Type strain: Yes

Species: Massilia oculi

Strain history: <- CCM <- CCUG <- Wristad, PHLS, Linköping, Sweden

NCBI tax ID(s): 945844 (species)

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General

@ref: 18192

BacDive-ID: 11339

DSM-Number: 26321

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Massilia oculi DSM 26321 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from human eye, endophthalmitis.

NCBI tax id

  • NCBI tax id: 945844
  • Matching level: species

strain history

  • @ref: 18192
  • history: <- CCM <- CCUG <- Wristad, PHLS, Linköping, Sweden

doi: 10.13145/bacdive11339.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia oculi
  • full scientific name: Massilia oculi Kämpfer et al. 2012

  • synonyms

    • @ref: 20215
    • synonym: Telluria oculi

@ref: 18192

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia oculi

full scientific name: Massilia oculi Kämpfer et al. 2012

type strain: yes

Morphology

cell morphology

  • @ref: 30283
  • gram stain: negative
  • cell length: 2 µm
  • cell width: 1 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 18192
  • type of hemolysis: gamma
  • incubation period: 1-2 days

pigmentation

  • @ref: 30283
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 18192
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18192positivegrowth30mesophilic
30283positivegrowth15-37

culture pH

  • @ref: 30283
  • ability: positive
  • type: growth
  • pH: 5.5-10.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 30283
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30283
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
30283370543-hydroxybutyrate+carbon source
3028316449alanine+carbon source
3028322599arabinose+carbon source
3028317234glucose+carbon source
3028326271proline+carbon source
3028317272propionate+carbon source
3028326546rhamnose+carbon source
3028317822serine+carbon source
3028353258sodium citrate+carbon source
302834853esculin+hydrolysis
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
18192catalase+1.11.1.6
18192cytochrome-c oxidase+1.9.3.1
30283cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
18192-----++-+++--++--+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
18192human eye, endophthalmitisLinköpingSwedenSWEEurope
55859Human eye,endophthalmitisLinköpingSwedenSWEEurope2000-03-06

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human
#Host Body-Site#Organ#Eye

Safety information

risk assessment

  • @ref: 18192
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18192
  • description: Massilia oculi partial 16S rRNA gene, type strain CCUG 43427AT
  • accession: FR773700
  • length: 1450
  • database: ena
  • NCBI tax ID: 945844

External links

@ref: 18192

culture collection no.: DSM 26321, CCM 7900, CCUG 43427 A

straininfo link

  • @ref: 80572
  • straininfo: 109598

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21441374Massilia oculi sp. nov., isolated from a human clinical specimen.Kampfer P, Lodders N, Martin K, Falsen EInt J Syst Evol Microbiol10.1099/ijs.0.032441-02011Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Endophthalmitis/*microbiology, Fatty Acids/analysis, Genes, rRNA, Gram-Negative Bacterial Infections/*microbiology, Humans, Lipids/analysis, Molecular Sequence Data, Oxalobacteraceae/chemistry/*classification/genetics/*isolation & purification, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Vitamin K 2/analysisPathogenicity
Phylogeny30443686Complete Genome Sequence of Massilia oculi sp. nov. CCUG 43427(T) (=DSM 26321(T)), the Type Strain of M. oculi, and Comparison with Genome Sequences of Other Massilia Strains.Song W, Wang S, Shen J, Zhu BCurr Microbiol10.1007/s00284-018-1597-72018Base Composition, Base Sequence, DNA, Bacterial/genetics, Endophthalmitis/*microbiology, *Genome, Bacterial, Gram-Negative Bacterial Infections/*microbiology, Humans, Oxalobacteraceae/classification/genetics/*isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Whole Genome SequencingGenetics
Phylogeny32559833Massilia arenae sp. nov., isolated from sand soil in the Qinghai-Tibetan Plateau.Zhang B, Yang R, Zhang G, Zhang D, Zhang W, Chen T, Liu GInt J Syst Evol Microbiol10.1099/ijsem.0.0040562020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sand/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Tibet, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18192Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26321)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26321
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30283Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2662428776041
55859Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43427 A)https://www.ccug.se/strain?id=43427
68369Automatically annotated from API 20NE
80572Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID109598.1StrainInfo: A central database for resolving microbial strain identifiers