Strain identifier

BacDive ID: 11332

Type strain: Yes

Species: Massilia haematophila

Strain history: <- P. Kämpfer, Univ. Giessen, Germany <- CCUG <- A. Wistedt, PHLS, Lund, Sweden

NCBI tax ID(s): 457923 (species)

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General

@ref: 16062

BacDive-ID: 11332

DSM-Number: 21946

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Massilia haematophila DSM 21946 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from human blood, 23-year-old man with multiple health problems.

NCBI tax id

  • NCBI tax id: 457923
  • Matching level: species

strain history

  • @ref: 16062
  • history: <- P. Kämpfer, Univ. Giessen, Germany <- CCUG <- A. Wistedt, PHLS, Lund, Sweden

doi: 10.13145/bacdive11332.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia haematophila
  • full scientific name: Massilia haematophila (Kämpfer et al. 2008) Kämpfer et al. 2011

  • synonyms

    @refsynonym
    20215Telluria haematophila
    20215Naxibacter haematophilus

@ref: 16062

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia haematophila

full scientific name: Massilia haematophila (Kämpfer et al. 2008) Kämpfer et al. 2011

type strain: yes

Morphology

cell morphology

  • @ref: 32451
  • gram stain: negative
  • cell length: 2 µm
  • cell width: 1 µm
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 32451
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16062
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16062positivegrowth28mesophilic
32451positivegrowth15-37
32451positiveoptimum25-30mesophilic

culture pH

@refabilitytypepHPH range
32451positivegrowth5.5-10.5alkaliphile
32451positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 32451
  • oxygen tolerance: aerobe

spore formation

  • @ref: 32451
  • spore formation: no

observation

  • @ref: 32451
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3245130089acetate+carbon source
3245117128adipate+carbon source
3245115361pyruvate+carbon source
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
32451cytochrome oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
16062------+------+--++-++
16062------+------+--++-++
16062------+------+--++-++
16062-------------+--++-++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
16062human blood, 23-year-old man with multiple health problemsSwedenSWEEurope
54191Human blood,23-yr-old man,multiple health problemsSwedenSWEEurope1997-08-01Lund

isolation source categories

Cat1Cat2Cat3
#Host#Human#Male
#Host Body Product#Fluids#Blood

Safety information

risk assessment

  • @ref: 16062
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16062
  • description: Naxibacter haematophilus partial 16S rRNA gene, type strain CCUG 38318T
  • accession: AM774589
  • length: 1433
  • database: ena
  • NCBI tax ID: 457923

External links

@ref: 16062

culture collection no.: DSM 21946, CCUG 38318, CCM 7480

straininfo link

  • @ref: 80565
  • straininfo: 17957

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18599716Naxibacter varians sp. nov. and Naxibacter haematophilus sp. nov., and emended description of the genus Naxibacter.Kampfer P, Falsen E, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.65516-02008Adult, Aged, 80 and over, Fatty Acids/metabolism, Humans, Japan, Male, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/chemistry/*classification/genetics/isolation & purification, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityPathogenicity
Phylogeny19783608Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples.Weon HY, Yoo SH, Kim SJ, Kim YS, Anandham R, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.015479-02009*Air Microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Oxalobacteraceae/*classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny25273514Massilia norwichensis sp. nov., isolated from an air sample.Orthova I, Kampfer P, Glaeser SP, Kaden R, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.068296-02014*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, *Phylogeny, Putrescine/analogs & derivatives/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, United KingdomGenetics
Phylogeny27334790Massiliapinisoli sp. nov., isolated from forest soil.Altankhuu K, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0012492016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome
Phylogeny28809142Massilia agri sp. nov., isolated from reclaimed grassland soil.Chaudhary DK, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0020022017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Grassland, Nepal, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome
Phylogeny28820094Massilia solisilvae sp. nov., Massilia terrae sp. nov. and Massilia agilis sp. nov., isolated from forest soil in South Korea by using a newly developed culture method.Altankhuu K, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0020762017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, Putrescine/chemistry, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16062Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21946)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21946
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32451Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2867328776041
54191Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38318)https://www.ccug.se/strain?id=38318
68369Automatically annotated from API 20NE
80565Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID17957.1StrainInfo: A central database for resolving microbial strain identifiers