General

@ref: 4258

BacDive-ID: 11302

DSM-Number: 11249

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Opitutus terrae ACB90 is an anaerobe, mesophilic bacterium that was isolated from anoxic rice paddy soil.

NCBI tax id

• NCBI tax id: 107709
• Matching level: species

strain history

• @ref: 4258
• history: <- K.-J. Chin, Max-Planck-Institut für terrestrische Mikrobiologie, Marburg, Germany; ACB90 <- K.-J. Chin {1994}

doi: 10.13145/bacdive11302.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/verrucomicrobiota
• domain: Bacteria
• phylum: Verrucomicrobiota
• class: Opitutia
• order: Opitutales
• family: Opitutaceae
• genus: Opitutus
• species: Opitutus terrae
• full scientific name: Opitutus terrae Chin et al. 2001

@ref: 4258

domain: Bacteria

phylum: Verrucomicrobia

class: Opitutae

order: Opitutales

family: Opitutaceae

genus: Opitutus

species: Opitutus terrae

full scientific name: Opitutus terrae Chin et al. 2001 emend. Hahnke et al. 2016 emend. Tegtmeier et al. 2018

strain designation: ACB90

type strain: no

Morphology

colony morphology

• @ref: 4258
• incubation period: 8-14 days

Culture and growth conditions

culture medium

• @ref: 4258
• name: OPITUTUS MEDIUM (DSMZ Medium 295)
• growth: yes
• link: https://mediadive.dsmz.de/medium/295
• composition: Name: OPITUTUS MEDIUM (DSMZ Medium 295; with strain-specific modifications) Composition: Cellobiose 0.997009 g/l Na2CO3 0.997009 g/l NaCl 0.997009 g/l NaNO3 0.797607 g/l KCl 0.498504 g/l MgCl2 x 6 H2O 0.398804 g/l L-Cysteine HCl x H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

• @ref: 4258
• growth: positive
• type: growth
• temperature: 28
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 4258
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 4258
• sample type: anoxic rice paddy soil
• geographic location: Vercelli
• country: Italy
• origin.country: ITA
• continent: Europe

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_6944.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_382;96_3265;97_4004;98_5108;99_6944&stattab=map
• Last taxonomy: Opitutus terrae subclade
• 16S sequence: AJ229246
• Sequence Identity:
• Total samples: 5688
• soil counts: 1953
• aquatic counts: 3265
• animal counts: 289
• plant counts: 181

Safety information

risk assessment

• @ref: 4258
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 4258
• description: Opitutus terrae 16S rRNA gene, strain ACB90
• accession: AJ229246
• length: 1480
• database: ena
• NCBI tax ID: 107709

External links

@ref: 4258

culture collection no.: DSM 11249

straininfo link

• @ref: 80535
• straininfo: 49056

Reference