Archive BacDiveID:10.13145/bacdive11279.20230509.8.1

Strain identifier

BacDive ID: 11279

Type strain:

Species: Marinospirillum insulare

Strain Designation: K

Strain history: <- LMG <- M. Satomi, Natl. Res. Inst. Fish. Sci., Yokohama, Japan <- T. Fujii, Tokyo Univ. Marine Sci. & Technol., Dept. Food Sci. & Technol.

NCBI tax ID(s): 1122211 (strain), 217169 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 15860

BacDive-ID: 11279

DSM-Number: 21763

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, coccus-shaped

description: Marinospirillum insulare K is an aerobe, mesophilic, Gram-negative bacterium that was isolated from fermented fish brine .

NCBI tax id

NCBI tax id	Matching level
1122211	strain
217169	species

strain history

@ref: 15860
history: <- LMG <- M. Satomi, Natl. Res. Inst. Fish. Sci., Yokohama, Japan <- T. Fujii, Tokyo Univ. Marine Sci. & Technol., Dept. Food Sci. & Technol.

doi: 10.13145/bacdive11279.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Oceanospirillales
family: Oceanospirillaceae
genus: Marinospirillum
species: Marinospirillum insulare
full scientific name: Marinospirillum insulare Satomi et al. 2004

@ref: 15860

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Marinospirillum

species: Marinospirillum insulare

full scientific name: Marinospirillum insulare Satomi et al. 2004

strain designation: K

type strain: yes

Morphology

cell morphology

@ref	gram stain	cell length	cell width	cell shape	motility	confidence
29973	negative	2.5-7.5 µm	0.1-0.2 µm	coccus-shaped	yes
69480					yes	93.28
69480	negative					99.998

pigmentation

@ref: 29973
production: yes

Culture and growth conditions

culture medium

@ref: 15860
name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
growth: yes
link: https://mediadive.dsmz.de/medium/514
composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
15860	positive	growth	28	mesophilic
29973	positive	growth	04-37
29973	positive	optimum	25-30	mesophilic

culture pH

@ref	ability	type	pH	PH range
29973	positive	growth	6.5-10.0	alkaliphile
29973	positive	optimum	8

Physiology and metabolism

oxygen tolerance

@ref: 29973
oxygen tolerance: aerobe

spore formation

@ref	spore formation	confidence
29973	no
69481	no	100
69480	no	99.899

halophily

@ref	salt	growth	tested relation	concentration
29973	NaCl	positive	growth	0.5-10 %
29973	NaCl	positive	optimum	02-03 %

observation

@ref: 29973
observation: aggregates in clumps

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
29973	16449	alanine	+	carbon source
29973	31011	valerate	+	carbon source
29973	17632	nitrate	+	reduction

enzymes

@ref	value	activity	ec
29973	alkaline phosphatase	+	3.1.3.1
29973	catalase	+	1.11.1.6
29973	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

isolation

@ref: 15860
sample type: fermented fish brine (kusaya gravy)
geographic location: Niijima Island
country: Japan
origin.country: JPN
continent: Asia

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Seafood
#Host	#Fishes
#Condition	#Saline
#Engineered	#Food production	#Fermented

taxonmaps

@ref: 69479
File name: preview.99_84839.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15849;96_1225;97_1452;98_1770;99_84839&stattab=map
Last taxonomy: Marinospirillum insulare
16S sequence: AB681133
Sequence Identity:
Total samples: 223
soil counts: 61
aquatic counts: 67
animal counts: 84
plant counts: 11

Safety information

risk assessment

@ref: 15860
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Marinospirillum insulare gene for 16S rRNA, partial sequence, strain: NBRC 100033	AB681133	1460	ena	217169
15860	Marinospirillum insulare gene for 16S rRNA, partial sequence, strain:K	AB098510	1432	ena	217169

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Marinospirillum insulare DSM 21763	GCA_000686905	scaffold	ncbi	1122211
66792	Marinospirillum insulare DSM 21763	1122211.3	wgs	patric	1122211
66792	Marinospirillum insulare DSM 21763	2563366729	draft	img	1122211

GC content

@ref: 29973
GC-content: 42.1

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	100	no
motile	yes	90.18	yes
flagellated	yes	73.407	no
gram-positive	no	99.126	yes
anaerobic	no	92.946	yes
halophile	yes	69.529	no
spore-forming	no	94.391	yes
glucose-util	no	67.767	no
aerobic	yes	75.281	yes
thermophile	no	99.049	yes
glucose-ferment	no	87.322	no

External links

@ref: 15860

culture collection no.: DSM 21763, LMG 21802, NBRC 100033

straininfo link

@ref: 80512
straininfo: 93907

literature

topic: Phylogeny
Pubmed-ID: 14742475
title: Marinospirillum insulare sp. nov., a novel halophilic helical bacterium isolated from kusaya gravy.
authors: Satomi M, Kimura B, Hayashi M, Okuzumi M, Fujii T
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.02768-0
year: 2004
mesh: Animals, DNA Gyrase/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Fishes, Food Microbiology, Molecular Sequence Data, Oceanospirillaceae/classification/genetics/isolation & purification/*ultrastructure, Phylogeny, Quinones/metabolism, RNA, Ribosomal, 16S/genetics, Salts
topic2: Metabolism

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed	ID_cross_reference
15860	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21763)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-21763
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
29973	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information		28776041	26342
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
80512	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID93907.1	StrainInfo: A central database for resolving microbial strain identifiers