Strain identifier
BacDive ID: 11050
Type strain:
Species: Rhodococcus nanhaiensis
Strain history: <- J. Li, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; SCSIO 10187
NCBI tax ID(s): 1081097 (species)
General
@ref: 17762
BacDive-ID: 11050
DSM-Number: 45608
keywords: 16S sequence, aerobe, mesophilic, Gram-positive
description: Rhodococcus nanhaiensis DSM 45608 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from marine sediment.
NCBI tax id
- NCBI tax id: 1081097
- Matching level: species
strain history
- @ref: 17762
- history: <- J. Li, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; SCSIO 10187
doi: 10.13145/bacdive11050.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- genus: Rhodococcus
- species: Rhodococcus nanhaiensis
- full scientific name: Rhodococcus nanhaiensis Li et al. 2012
@ref: 17762
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardiaceae
genus: Rhodococcus
species: Rhodococcus nanhaiensis
full scientific name: Rhodococcus nanhaiensis Li et al. 2012
type strain: yes
Morphology
cell morphology
- @ref: 30525
- gram stain: positive
- motility: no
colony morphology
@ref | colony color | medium used |
---|---|---|
69412 | Honey yellow (1005) | suter without tyrosine |
69412 | Maize yellow (1006) | ISP 7 |
69412 | Sand yellow (1002) | ISP 2 |
69412 | Sand yellow (1002) | ISP 4 |
69412 | Sand yellow (1002) | ISP 5 |
69412 | Sand yellow (1002), brown beige (1011) | suter with tyrosine |
multicellular morphology
@ref | forms multicellular complex | complex name | medium name |
---|---|---|---|
69412 | no | Aerial mycelium | ISP 2 |
69412 | no | Aerial mycelium | ISP 3 |
69412 | no | Aerial mycelium | ISP 4 |
69412 | no | Aerial mycelium | ISP 5 |
69412 | no | Aerial mycelium | ISP 6 |
69412 | no | Aerial mycelium | ISP 7 |
69412 | no | Aerial mycelium | suter with tyrosine |
69412 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name |
---|---|---|
30525 | yes | |
69412 | no | soluble pigment |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
17762 | https://www.dsmz.de/microorganisms/photos/DSM_45608.jpg | Medium 535 28°C | © Leibniz-Institut DSMZ |
69412 | DSM_45608_image3.jpeg | Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69412 | DSM_45608_image4.jpeg | Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
- @ref: 17762
- name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535
- composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17762 | positive | growth | 28 | mesophilic |
30525 | positive | growth | 15-35 | |
30525 | positive | optimum | 28 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
30525 | positive | growth | 05-08 |
30525 | positive | optimum | 6 |
Physiology and metabolism
oxygen tolerance
- @ref: 30525
- oxygen tolerance: aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
30525 | NaCl | positive | growth | 0-10 % |
30525 | NaCl | positive | optimum | 5 % |
69412 | NaCl | positive | growth | 0-2.5 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69412 | 22599 | arabinose | - | growth |
30525 | 28757 | fructose | + | carbon source |
69412 | 62968 | cellulose | - | growth |
69412 | 28757 | fructose | + | growth |
69412 | 17234 | glucose | - | growth |
69412 | 17268 | inositol | + | growth |
69412 | 37684 | mannose | - | growth |
69412 | 16634 | raffinose | - | growth |
69412 | 26546 | rhamnose | - | growth |
69412 | 17992 | sucrose | - | growth |
69412 | 18222 | xylose | - | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30525 | catalase | + | 1.11.1.6 |
30525 | cytochrome oxidase | + | 1.9.3.1 |
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | trypsin | - | 3.4.21.4 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69412 | - | - | - | + | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69412 | + | +/- | + | - | + | + | +/- | - | +/- | + | + | - | - | - | + | +/- | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 17762
- sample type: marine sediment
- geographic location: South China Sea (20° 44.8951' N 114° 15.2932' E) at a depth of 84.5 m
- country: China
- origin.country: CHN
- continent: Asia
- latitude: 20.7483
- longitude: 114.255
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 17762
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17762
- description: Rhodococcus nanhaiensis strain SCSIO 10187 16S ribosomal RNA gene, partial sequence
- accession: JN582175
- length: 1400
- database: ena
- NCBI tax ID: 1081097
GC content
@ref | GC-content | method |
---|---|---|
17762 | 63.7 | high performance liquid chromatography (HPLC) |
30525 | 63.7 |
External links
@ref: 17762
culture collection no.: DSM 45608, CCTCC AB 2011024, SCSIO 10187
straininfo link
- @ref: 80300
- straininfo: 405307
literature
- topic: Phylogeny
- Pubmed-ID: 22180608
- title: Rhodococcus nanhaiensis sp. nov., an actinobacterium isolated from marine sediment.
- authors: Li J, Zhao GZ, Long LJ, Wang FZ, Tian XP, Zhang S, Li WJ
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.038067-0
- year: 2011
- mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Oceans and Seas, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodococcus/*classification/genetics/isolation & purification, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
17762 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45608) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45608 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30525 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26857 | 28776041 | |
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69412 | Wink, J. | https://cdn.dsmz.de/wink/DSM%2045608.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
80300 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405307.1 | StrainInfo: A central database for resolving microbial strain identifiers |