Strain identifier
BacDive ID: 11048
Type strain:
Species: Rhodococcus artemisiae
Strain history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65754 <- J. Li, Yunnan Univ., Kunming
NCBI tax ID(s): 714159 (species)
General
@ref: 16418
BacDive-ID: 11048
DSM-Number: 45380
keywords: 16S sequence, aerobe, mesophilic, Gram-positive, ovoid-shaped
description: Rhodococcus artemisiae DSM 45380 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from surface-sterilized stem of Artemisia annua L..
NCBI tax id
- NCBI tax id: 714159
- Matching level: species
strain history
- @ref: 16418
- history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65754 <- J. Li, Yunnan Univ., Kunming
doi: 10.13145/bacdive11048.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- genus: Rhodococcus
- species: Rhodococcus artemisiae
- full scientific name: Rhodococcus artemisiae Zhao et al. 2012
@ref: 16418
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardiaceae
genus: Rhodococcus
species: Rhodococcus artemisiae
full scientific name: Rhodococcus artemisiae Zhao et al. 2012
type strain: yes
Morphology
cell morphology
- @ref: 30260
- gram stain: positive
- cell shape: ovoid-shaped
- motility: no
colony morphology
@ref | colony color | medium used |
---|---|---|
69390 | Beige (1001) | ISP 2 |
69390 | Beige (1001) | ISP 6 |
69390 | Ivory (1014) | suter with tyrosine |
69390 | Light ivory (1015) | ISP 4 |
69390 | Light ivory (1015) | ISP 7 |
69390 | Light ivory (1015) | suter without tyrosine |
69390 | Oyster white (1013) | ISP 3 |
69390 | Oyster white (1013) | ISP 5 |
multicellular morphology
@ref | forms multicellular complex | complex name | medium name |
---|---|---|---|
69390 | no | Aerial mycelium | ISP 2 |
69390 | no | Aerial mycelium | ISP 3 |
69390 | no | Aerial mycelium | ISP 4 |
69390 | no | Aerial mycelium | ISP 5 |
69390 | no | Aerial mycelium | ISP 6 |
69390 | no | Aerial mycelium | ISP 7 |
69390 | no | Aerial mycelium | suter with tyrosine |
69390 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name |
---|---|---|
30260 | yes | |
69390 | no | Melanin |
69390 | no | soluble pigment |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
69390 | DSM_45380_image3.jpeg | Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69390 | DSM_45380_image4.jpeg | Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
16418 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
16418 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16418 | positive | growth | 28 | mesophilic |
30260 | positive | growth | 10-40 | |
30260 | positive | optimum | 20-37 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30260 | positive | growth | 06-09 | alkaliphile |
30260 | positive | optimum | 07-08 |
Physiology and metabolism
oxygen tolerance
- @ref: 30260
- oxygen tolerance: aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
30260 | NaCl | positive | growth | 0-7 % |
69390 | NaCl | positive | growth | 0-5 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30260 | 22599 | arabinose | + | carbon source |
30260 | 17057 | cellobiose | + | carbon source |
30260 | 28757 | fructose | + | carbon source |
30260 | 28260 | galactose | + | carbon source |
30260 | 17234 | glucose | + | carbon source |
30260 | 17754 | glycerol | + | carbon source |
30260 | 17306 | maltose | + | carbon source |
30260 | 29864 | mannitol | + | carbon source |
30260 | 37684 | mannose | + | carbon source |
30260 | 26546 | rhamnose | + | carbon source |
30260 | 33942 | ribose | + | carbon source |
30260 | 30911 | sorbitol | + | carbon source |
30260 | 17992 | sucrose | + | carbon source |
30260 | 53424 | tween 20 | + | carbon source |
30260 | 53423 | tween 40 | + | carbon source |
30260 | 18222 | xylose | + | carbon source |
69390 | 22599 | arabinose | - | growth |
69390 | 62968 | cellulose | - | growth |
69390 | 28757 | fructose | + | growth |
69390 | 17234 | glucose | + | growth |
69390 | 17268 | inositol | - | growth |
69390 | 37684 | mannose | + | growth |
69390 | 16634 | raffinose | - | growth |
69390 | 26546 | rhamnose | - | growth |
69390 | 17992 | sucrose | - | growth |
69390 | 18222 | xylose | - | growth |
68379 | 17632 | nitrate | + | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30260 | catalase | + | 1.11.1.6 |
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69390 | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69390 | +/- | +/- | + | +/- | + | +/- | +/- | +/- | - | + | + | - | - | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 16418
- sample type: surface-sterilized stem of Artemisia annua L.
- host species: Artemisia annua
- geographic location: Yunnan province
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Stem (Branch) |
#Host Body-Site | #Plant | #Sterilized plant part |
Safety information
risk assessment
- @ref: 16418
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16418
- description: Rhodococcus artemisiae strain YIM 65754 16S ribosomal RNA gene, partial sequence
- accession: GU367155
- length: 1516
- database: ena
- NCBI tax ID: 714159
GC content
@ref | GC-content | method |
---|---|---|
16418 | 66.2 | high performance liquid chromatography (HPLC) |
30260 | 66.2 |
External links
@ref: 16418
culture collection no.: DSM 45380, CCTCC AA 209042, YIM 65754
straininfo link
- @ref: 80298
- straininfo: 398962
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 21642485 | Rhodococcus artemisiae sp. nov., an endophytic actinobacterium isolated from the pharmaceutical plant Artemisia annua L. | Zhao GZ, Li J, Zhu WY, Tian SZ, Zhao LX, Yang LL, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.031930-0 | 2011 | Artemisia annua/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Rhodococcus/*classification/genetics/isolation & purification, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Enzymology |
Phylogeny | 26310241 | Rhodococcus lactis sp. nov., an actinobacterium isolated from sludge of a dairy waste treatment plant. | Singh PK, Kumari A, Chawla N, Pinnaka AK, Korpole S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000565 | 2015 | Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, *Dairying, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodococcus/*classification/genetics/isolation & purification, Sequence Analysis, DNA, Sewage/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
16418 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45380) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45380 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30260 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26601 | 28776041 | |
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69390 | Wink, J. | https://cdn.dsmz.de/wink/DSM%2045380.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
80298 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID398962.1 | StrainInfo: A central database for resolving microbial strain identifiers |