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Strain identifier

BacDive ID: 10892

Type strain: Yes

Species: Rhodococcus marinonascens

Strain Designation: 3438W

Strain history: DSM 43752 <-- H. Weyland 3438W.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11249

BacDive-ID: 10892

DSM-Number: 43752

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Rhodococcus marinonascens 3438W is a mesophilic bacterium that was isolated from marine sediment.

NCBI tax id

NCBI tax idMatching level
1219020strain
38311species

strain history

doi: 10.13145/bacdive10892.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Rhodococcus
  • species: Rhodococcus marinonascens
  • full scientific name: Rhodococcus marinonascens Helmke and Weyland 1984

@ref: 11249

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus marinonascens

full scientific name: Rhodococcus marinonascens Helmke and Weyland 1984

strain designation: 3438W

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 96.305

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11249ARTIFICIAL SEA WATER – TRYPTONE SOY BROTH MEDIUM (ASW TSB MEDIUM) (DSMZ Medium 1691)yeshttps://mediadive.dsmz.de/medium/1691Name: Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium) (DSMZ Medium 1691) Composition: NaCl 27.2 g/l Agar 20.0 g/l Trypticase peptone 17.0 g/l MgSO4 x 7 H2O 11.88 g/l MgCl2 x 6 H2O 9.06 g/l Soy peptone 3.0 g/l CaCl2 x 2 H2O 2.6 g/l D-Glucose 2.5 g/l KCl 1.28 g/l Na2HPO4 x 2 H2O 0.02 g/l NH4NO3 0.0042 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Double distilled water Distilled water
11249LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381; with strain-specific modifications) Composition: NaCl 30.0 g/l Agar 20.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
11249SEA WATER AGAR (DSMZ Medium 246)yeshttps://mediadive.dsmz.de/medium/246Name: SEA WATER AGAR (DSMZ Medium 246) Composition: Agar 20.0 g/l Peptone 10.0 g/l Beef extract 10.0 g/l Tap water Sea water
34041Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)

culture temp

@refgrowthtypetemperaturerange
11249positivegrowth28mesophilic
11249positivegrowth18psychrophilic
34041positivegrowth18psychrophilic
67770positivegrowth18psychrophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 69.558

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 90.328

Isolation, sampling and environmental information

isolation

@refsample type
11249marine sediment
67770Marine sediment

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_6550.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_181;97_445;98_509;99_6550&stattab=map
  • Last taxonomy: Rhodococcus marinonascens
  • 16S sequence: X80617
  • Sequence Identity:
  • Total samples: 1517
  • soil counts: 1055
  • aquatic counts: 143
  • animal counts: 192
  • plant counts: 127

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112491Risk group (German classification)
195841Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218R.marinonanscens 16S rRNA gene (ATCC 35653T)X819331315ena38311
20218Rhodococcus marinonascens 16S rRNA gene, strain DSM43752TX806171474ena38311

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Rhodococcus marinonascens NBRC 143631219020.3wgspatric1219020
66792Rhodococcus marinonascens NBRC 143632867152395draftimg1219020
67770Rhodococcus marinonascens NBRC 14363 NBRC 14363GCA_001894885contigncbi1219020
66792Klebsiella pneumoniae SIKP188GCA_004833185contigpatric573

GC content

@refGC-contentmethod
1124964.9
6777064.9thermal denaturation, midpoint method (Tm)

External links

@ref: 11249

culture collection no.: DSM 43752, ATCC 35653, IFO 14363, JCM 6241, NBRC 14363, NCMB 2246, BCRC 13696, CECT 4621, CGMCC 4.1726, CIP 104177, KCTC 9809, NCIMB 2246, NRRL B-16940, VKM Ac-1182

straininfo link

@refpassport
20218http://www.straininfo.net/strains/111020
20218http://www.straininfo.net/strains/111019
20218http://www.straininfo.net/strains/154614
20218http://www.straininfo.net/strains/154615
20218http://www.straininfo.net/strains/335151
20218http://www.straininfo.net/strains/168632

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11249Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43752)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43752
19584Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43752.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34041Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16057
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)