Strain identifier

BacDive ID: 10829

Type strain: Yes

Species: Nocardia artemisiae

Strain history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65623 <- J. Li, Yunnan Univ., Kunming

NCBI tax ID(s): 714130 (species)

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General

@ref: 16417

BacDive-ID: 10829

DSM-Number: 45379

keywords: 16S sequence, Bacteria, mesophilic

description: Nocardia artemisiae DSM 45379 is a mesophilic bacterium that builds an aerial mycelium and was isolated from healthy inner tissue of the stem of Artemisia annua, a traditional Chinese medicinal plant.

NCBI tax id

  • NCBI tax id: 714130
  • Matching level: species

strain history

  • @ref: 16417
  • history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65623 <- J. Li, Yunnan Univ., Kunming

doi: 10.13145/bacdive10829.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia artemisiae
  • full scientific name: Nocardia artemisiae Zhao et al. 2011

@ref: 16417

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Nocardia

species: Nocardia artemisiae

full scientific name: Nocardia artemisiae Zhao et al. 2011

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69389Fawn brown (8007)suter with tyrosine
69389Clay brown (8003)ISP 3
69389Clay brown (8003)ISP 4
69389Chocolate brown (8017)ISP 7
69389Brown beige (1011)ISP 6
69389Brown beige (1011)suter without tyrosine
69389Pale brown (8025)ISP 5
69389Saffron yellow (1017)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69389yesAerial myceliumCream (9001)ISP 2
69389noAerial myceliumISP 3
69389noAerial myceliumISP 4
69389yesAerial myceliumCream (9001)ISP 5
69389yesAerial myceliumPure white (9010)ISP 7
69389yesAerial myceliumBeige (1001)suter with tyrosine
69389yesAerial myceliumBeige (1001)suter without tyrosine

pigmentation

@refproductionnamecolor
69389yesMelanin
69389yessoluble pigmentClay brown (8003), ocher brown (8001)

multimedia

@refmultimedia contentcaptionintellectual property rights
69389DSM_45379_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69389DSM_45379_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

  • @ref: 16417
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

  • @ref: 16417
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69389
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2.5-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
6938922599arabinose-growth
6938962968cellulose-growth
6938928757fructose-growth
6938917234glucose+growth
6938917268inositol+/-growth
6938937684mannose-growth
6938916634raffinose-growth
6938926546rhamnose-growth
6938917992sucrose+/-growth
6938918222xylose-growth
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69389++-+--+-++---------

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
16417-+--------+----++---
69389++/-+/--+----++-+/-+/-++--+/-

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
16417+----+--+/---------++-

Isolation, sampling and environmental information

isolation

  • @ref: 16417
  • sample type: healthy inner tissue of the stem of Artemisia annua, a traditional Chinese medicinal plant
  • host species: Artemisia annua
  • geographic location: Yunnan
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Stem (Branch)

Safety information

risk assessment

  • @ref: 16417
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16417
  • description: Nocardia artemisiae strain YIM 65623 16S ribosomal RNA gene, partial sequence
  • accession: GU367157
  • length: 1511
  • database: ena
  • NCBI tax ID: 714130

GC content

  • @ref: 16417
  • GC-content: 69.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 16417

culture collection no.: DSM 45379, CCTCC AA 209038, YIM 65623

straininfo link

  • @ref: 80090
  • straininfo: 378832

literature

  • topic: Phylogeny
  • Pubmed-ID: 21278414
  • title: Nocardia artemisiae sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of Artemisia annua L.
  • authors: Zhao GZ, Li J, Zhu WY, Klenk HP, Xu LH, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029306-0
  • year: 2011
  • mesh: Artemisia annua/*microbiology, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nocardia/*classification/genetics/*isolation & purification/metabolism, Phylogeny, Plant Stems/*microbiology, RNA, Ribosomal, 16S/genetics, Sodium Chloride/metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
16417Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45379)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45379
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69389Wink, J.https://cdn.dsmz.de/wink/DSM%2045379.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
80090Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID378832.1StrainInfo: A central database for resolving microbial strain identifiers