Strain identifier

BacDive ID: 1081

Type strain: Yes

Species: Sutcliffiella cohnii

Strain Designation: RSH

Strain history: CIP <- 1995, DSM <- NCIMB <- M.E. Chislett: strain RU 38, Bacillus circulans

NCBI tax ID(s): 1314751 (strain), 33932 (species)

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General

@ref: 2589

BacDive-ID: 1081

DSM-Number: 6307

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile

description: Sutcliffiella cohnii RSH is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
33932species
1314751strain

strain history

@refhistory
2589<- R. Spanka; RSH
67770DSM 6307 <-- R. Spanka strain RSH.
118329CIP <- 1995, DSM <- NCIMB <- M.E. Chislett: strain RU 38, Bacillus circulans

doi: 10.13145/bacdive1081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Sutcliffiella
  • species: Sutcliffiella cohnii
  • full scientific name: Sutcliffiella cohnii (Spanka and Fritze 1993) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus cohnii

@ref: 2589

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Sutcliffiella

species: Sutcliffiella cohnii

full scientific name: Sutcliffiella cohnii (Spanka and Fritze 1993) Gupta et al. 2020

strain designation: RSH

type strain: yes

Morphology

cell morphology

@refgram staincell widthmotilityflagellum arrangementconfidencecell shape
23300positive0.6-0.7 µmyesperitrichous
69480yes96.089
69480positive100
118329positiveyesrod-shaped

colony morphology

  • @ref: 23300
  • colony size: 1-2 mm
  • colony color: cream white
  • incubation period: 2 days
  • medium used: alkaline meat extract-peptone

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2589ALKALINE NUTRIENT AGAR (DSMZ Medium 31)yeshttps://mediadive.dsmz.de/medium/31Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
23300alkaline meat extract-peptoneyes
35231MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
118329CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
2589positivegrowth30mesophilic
23300positivemaximum47thermophilic
23300positiveminimum10psychrophilic
35231positivegrowth30mesophilic
67770positivegrowth30mesophilic
118329positivegrowth30-45
118329nogrowth10psychrophilic
118329nogrowth55thermophilic

culture pH

  • @ref: 118329
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 118329
  • oxygen tolerance: obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23300oval and terminally locatedendosporeyes
69481yes100
69480yes100
118329yes

halophily

@refsaltgrowthtested relationconcentration
23300NaClpositivegrowth5 %
23300NaClnogrowth10 %

murein

  • @ref: 2589
  • murein short key: A21.04
  • type: A4ß L-Orn-D-Asp

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2330028044phenylalanine-assimilation
2330016199urea-hydrolysis
2330019044-methylumbelliferone beta-d-glucuronide+hydrolysis
23300casein+hydrolysis
233005291gelatin+hydrolysis
23300606565hippurate+hydrolysis
2330027941pullulan+hydrolysis
2330028017starch+hydrolysis
2330053424tween 20+hydrolysis
2330053423tween 40+hydrolysis
2330053425tween 60+hydrolysis
2330053426tween 80+hydrolysis
2330017632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11832916947citrate-carbon source
11832917632nitrate+reduction
11832916301nitrite-reduction
11832917632nitrate+respiration

metabolite production

  • @ref: 118329
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 118329
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
23300catalase+1.11.1.6
23300cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
118329oxidase-
118329alcohol dehydrogenase-1.1.1.1
118329gelatinase+
118329amylase-
118329DNase+
118329caseinase+3.4.21.50
118329catalase+1.11.1.6
118329lecithinase+
118329lysine decarboxylase-4.1.1.18
118329ornithine decarboxylase-4.1.1.17
118329urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118329-+++-+-----+--+++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118329------------------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2589soilBraunschweigGermanyDEUEurope
23300Soil, horse meadow
67770Soil, horse meadowBraunschweigGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Mammals#Equidae (Horse)

taxonmaps

  • @ref: 69479
  • File name: preview.99_7611.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_567;97_659;98_5573;99_7611&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: X76437
  • Sequence Identity:
  • Total samples: 1009
  • soil counts: 313
  • aquatic counts: 147
  • animal counts: 252
  • plant counts: 297

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
25891Risk group (German classification)
1183291Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218B.cohnii (DSM 6307 T) gene for 16S rRNAX764371506ena33932
20218Bacillus cohnii gene for 16S rRNA, partial sequence, strain: NBRC 15565AB6809031477ena33932

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sutcliffiella cohnii DSM 6307GCA_002250055completencbi33932
66792Bacillus cohnii NBRC 155651314751.3wgspatric1314751
66792Bacillus cohnii strain DSM 630733932.3completepatric33932
66792Sutcliffiella cohnii DSM 63072773857892completeimg33932
66792Bacillus cohnii NBRC 155652731957650draftimg1314751
67770Sutcliffiella cohnii NBRC 15565GCA_001591425contigncbi1314751

GC content

@refGC-contentmethod
258934.6
6777034.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes87.049yes
gram-positiveyes88.907no
anaerobicno98.436no
aerobicyes85.834no
halophileyes59.772no
spore-formingyes95.784yes
glucose-utilyes84.509no
thermophileno98.258no
motileyes88.603yes
glucose-fermentno92.806no

External links

@ref: 2589

culture collection no.: DSM 6307, ATCC 51227, CCM 4369, IFO 15565, NBRC 15565, JCM 12300, CGMCC 1.3671, CIP 104587, HAMBI 2098, KCTC 3572, LMG 16678, VTT E-011772

straininfo link

  • @ref: 70753
  • straininfo: 8356

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8323866Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall.Spanka R, Fritze DInt J Syst Bacteriol10.1099/00207713-43-1-1501993Amino Acid Sequence, Animals, Aspartic Acid/*analysis, Bacillus/chemistry/*classification/genetics/ultrastructure, Base Composition, Cell Wall/chemistry, DNA, Bacterial/analysis, Fatty Acids/analysis, Genotype, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Ornithine/*analysis, Phenotype, Sequence Homology, Nucleic Acid, Spores, Bacterial, Vitamin K/analogs & derivatives/analysis, *Vitamin K 2/*analogs & derivativesGenetics
Phylogeny15280316Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India.Suresh K, Prabagaran SR, Sengupta S, Shivaji SInt J Syst Evol Microbiol10.1099/ijs.0.03047-02004Anti-Bacterial Agents/pharmacology, Arsenic/*pharmacology, Bacillus/*classification/drug effects/*isolation & purification/physiology, DNA, Bacterial/analysis/chemistry/isolation & purification, DNA, Ribosomal/chemistry, Drug Resistance, Bacterial, Fatty Acids/analysis, Genes, rRNA, Gentian Violet, India, Molecular Sequence Data, Movement, Peptidoglycan/chemistry, Phenazines, Phylogeny, Pigments, Biological/biosynthesis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, *Soil Microbiology, Spores, Bacterial/cytology, Vitamin K 2/analysis, Water MicrobiologyEnzymology
Phylogeny18984705Bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.Lee JC, Lee GS, Park DJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.65733-02008Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Korea, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Silicon Dioxide, *Soil Microbiology, Species SpecificityGenetics
Phylogeny28744555Bacillus catenulatus sp. nov., an alkalitolerant bacterium isolated from a soda lake.Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VRArch Microbiol10.1007/s00203-017-1413-y2017*Bacillus/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition/genetics, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, India, Lakes/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylethanolamines/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/analysis, Spores, Bacterial/physiologyTranscriptome
Phylogeny35195511Sutcliffiella deserti sp. nov., isolated from desert soil.Bai P, Zhang S, Chen Y, Ping W, Pang H, Du J, Li Y, Zhang JInt J Syst Evol Microbiol10.1099/ijsem.0.0052592022*Bacillaceae/classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Mongolia, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2589Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6307)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6307
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23300Roland Spanka, Dagmar Fritze10.1099/00207713-43-1-150Bacillus cohnii sp. nov., a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell WallIJSEM 43: 150-156 19938323866
35231Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16512
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70753Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8356.1StrainInfo: A central database for resolving microbial strain identifiers
118329Curators of the CIPCollection of Institut Pasteur (CIP 104587)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104587