Strain identifier

BacDive ID: 10760

Type strain: Yes

Species: Nocardia veterana

Strain Designation: M157222

Strain history: CIP <- 2001, R. M. Kroppenstedt, Germany <- V. Gürtler

NCBI tax ID(s): 1206743 (strain), 132249 (species)

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General

@ref: 11772

BacDive-ID: 10760

DSM-Number: 44445

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Nocardia veterana M157222 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from a patient in the Austin & Repatrriation Medial Centre .

NCBI tax id

NCBI tax idMatching level
132249species
1206743strain

strain history

@refhistory
11772<- V. Gürtler, A & RMC; M157222
67770NRRL B-24136 <-- V. Gürtler M157222.
123832CIP <- 2001, R. M. Kroppenstedt, Germany <- V. Gürtler

doi: 10.13145/bacdive10760.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia veterana
  • full scientific name: Nocardia veterana Gürtler et al. 2001

@ref: 11772

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Nocardia

species: Nocardia veterana

full scientific name: Nocardia veterana Gürtler et al. 2001 emend. Nouioui et al. 2018

strain designation: M157222

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
123832positiverod-shapedno
125438no90
125438positive92.2
125439no91
125439positive98.3

colony morphology

@refcolony colorincubation periodmedium used
19668Rose10-14 daysISP 2
19668Beige10-14 daysISP 3
19668Rose10-14 daysISP 4
19668Beige10-14 daysISP 5
19668Beige10-14 daysISP 6
19668Beige10-14 daysISP 7
123832

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19668yesAerial MyceliumWhiteISP 2
19668yesAerial MyceliumWhiteISP 3
19668noISP 4
19668yesAerial MyceliumISP 5
19668noISP 6
19668yesAerial MyceliumWhiteISP 7

multimedia

  • @ref: 11772
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44445.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11772TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11772GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19668ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19668ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19668ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19668ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19668ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19668ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33811MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
33047MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
123832CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123832CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
19668positiveoptimum28
11772positivegrowth28
33047positivegrowth30
33811positivegrowth37
57069positivegrowth37-42
67770positivegrowth28
123832positivegrowth25-45
123832nogrowth10

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
57069aerobe
123832obligate aerobe
125439obligate aerobe98.7

halophily

@refsaltgrowthtested relationconcentration
123832NaClpositivegrowth0-6 %
123832NaClnogrowth8 %
123832NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(II,III-H4, ω-cycl)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1966817234glucose+
1966822599arabinose-
1966817992sucrose-
1966818222xylose+
1966817268myo-inositol+
1966829864mannitol-
1966828757fructose-
1966826546rhamnose+
1966816634raffinose+
1966862968cellulose+
12383216947citrate-carbon source
1238324853esculin-hydrolysis
123832606565hippurate-hydrolysis
12383217632nitrate-reduction
12383216301nitrite-reduction
12383217632nitrate-respiration
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

antibiotic resistance

  • @ref: 123832
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12383235581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole test
12383215688acetoin-
12383217234glucose-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
123832oxidase-
123832beta-galactosidase+3.2.1.23
123832alcohol dehydrogenase-1.1.1.1
123832gelatinase-
123832amylase-
123832DNase-
123832caseinase-3.4.21.50
123832catalase+1.11.1.6
123832tween esterase-
123832gamma-glutamyltransferase-2.3.2.2
123832lecithinase-
123832lipase-
123832lysine decarboxylase-4.1.1.18
123832ornithine decarboxylase-4.1.1.17
123832phenylalanine ammonia-lyase-4.3.1.24
123832protease-
123832tryptophan deaminase-
123832urease-3.5.1.5
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382lipase (C 14)-
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19668+-+-++---++-+-++---
123832-++-+----++----+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19668-++++------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123832---------+/---------------+/------------------------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123832++----------------+++----------------------------+---------+---------------+---++-------+-+----++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11772a patient in the Austin & Repatrriation Medial Centre (A & RMC)MelbourneAustraliaAUSAustralia and Oceania
67770Bronchoscopic lavage of a 78-year-old manAustraliaAUSAustralia and Oceania
123832HumanAustraliaAUSAustralia and Oceania

isolation source categories

  • Cat1: #Infection
  • Cat2: #Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_1641.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_243;97_1088;98_1298;99_1641&stattab=map
  • Last taxonomy: Nocardia
  • 16S sequence: GQ376181
  • Sequence Identity:
  • Total samples: 4172
  • soil counts: 2461
  • aquatic counts: 606
  • animal counts: 719
  • plant counts: 386

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
117722Risk group (German classification)
196681Risk group (German classification)
1238322Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Nocardia veterana strain DSM 44445 16S ribosomal RNA gene, complete sequenceAF4300551494nuccore132249
20218Nocardia veterana 16S ribosomal RNA gene, partial sequenceAY1710391471nuccore132249
20218Nocardia veterana strain DSM 44445T 16S ribosomal RNA gene, partial sequenceAY1912531395nuccore132249
20218Nocardia veterana strain DSM 44445 16S ribosomal RNA gene, partial sequenceAY756566606nuccore132249
20218Nocardia veterana strain DSM 44445 16S ribosomal RNA gene, partial sequenceDQ6599181391nuccore132249
20218Nocardia veterana 16S ribosomal RNA gene, partial sequenceGQ3761811441nuccore132249
20218Nocardia veterana strain NRRL B-24136 16S ribosomal RNA gene, partial sequenceJN041602462nuccore132249
11772Nocardia veterana 16S ribosomal RNA gene, partial sequenceAF2785721340nuccore132249
124043Nocardia veterana strain DSM 44445 16S ribosomal RNA gene, partial sequence.MW5638571364nuccore132249

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nocardia veterana NBRC 1003441206743.4wgspatric1206743
66792Nocardia veterana strain DSM 44445132249.3wgspatric132249
66792Nocardia veterana NBRC 1003442551306183draftimg1206743
67770Nocardia veterana NBRC 100344GCA_000308855contigncbi1206743
67770Nocardia veterana DSM 44445GCA_012396305contigncbi132249

GC content

  • @ref: 67770
  • GC-content: 68.2
  • method: genome sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe98.7
125439BacteriaNetgram_stainReaction to gram-stainingpositive98.3
125439BacteriaNetmotilityAbility to perform movementno91
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes88.5
125438gram-positivegram-positivePositive reaction to Gram-stainingyes92.2no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.917yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes63.238no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.86yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno96yes
125438motile2+flagellatedAbility to perform flagellated movementno90no

External links

@ref: 11772

culture collection no.: DSM 44445, CIP 107095, KCTC 19902, NRRL B-24136, CCUG 46118, JCM 11307, IFM 10086, NBRC 100344

straininfo link

  • @ref: 80028
  • straininfo: 50631

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411717Nocardia veterana sp. nov., isolated from human bronchial lavage.Gurtler V, Smith R, Mayall BC, Potter-Reinemann G, Stackebrandt E, Kroppenstedt RMInt J Syst Evol Microbiol10.1099/00207713-51-3-9332001Aged, Bronchoalveolar Lavage Fluid/*microbiology, DNA, Ribosomal/genetics, Fatty Acids/analysis, Fatty Acids, Nonesterified/analysis, Humans, Male, Molecular Sequence Data, Monosaccharides/metabolism, Nocardia/*classification/genetics/isolation & purification, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, VictoriaPathogenicity
Phylogeny32897850Nocardia aurantiaca sp. nov., isolated from soil in Thailand.Kanchanasin P, Yuki M, Kudo T, Ohkuma M, Phongsopitanun W, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0044322020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nocardia/*classification/isolation & purification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11772Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44445)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44445
19668Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44445.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33047Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19297
33811Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4939
57069Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46118)https://www.ccug.se/strain?id=46118
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80028Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50631.1StrainInfo: A central database for resolving microbial strain identifiers
123832Curators of the CIPCollection of Institut Pasteur (CIP 107095)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107095
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1