Strain identifier

BacDive ID: 10624

Type strain: Yes

Species: Gordonia phosphorivorans

Strain Designation: Ca8

Strain history: CIP <- 2011, P. Kämpfer, Giessen Univ., Giessen, Germany: strain Ca8

NCBI tax ID(s): 1056982 (species)

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General

@ref: 17921

BacDive-ID: 10624

DSM-Number: 45630

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Gordonia phosphorivorans Ca8 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from wastewater treatment bioreactor.

NCBI tax id

  • NCBI tax id: 1056982
  • Matching level: species

strain history

@refhistory
379342011, P. Kämpfer, Inst. Angewandte Mikrobiol., Giessen, Germany: strain Ca8
17921<- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; Ca8 <- J. Zimmermann, RWTH Aachen, Germany
67770CCM 7957 <-- P. Kämpfer; Justus-Liebig-Univ. Giessen, Germany; Ca8.
121809CIP <- 2011, P. Kämpfer, Giessen Univ., Giessen, Germany: strain Ca8

doi: 10.13145/bacdive10624.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Gordoniaceae
  • genus: Gordonia
  • species: Gordonia phosphorivorans
  • full scientific name: Gordonia phosphorivorans Kämpfer et al. 2013

@ref: 17921

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Gordoniaceae

genus: Gordonia

species: Gordonia phosphorivorans

full scientific name: Gordonia phosphorivorans Kämpfer et al. 2013

strain designation: Ca8

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30482positive1.25 µm1.25 µmcoccus-shaped
121809positiverod-shapedno

colony morphology

  • @ref: 62648
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37934MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
17921TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
121809CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328
121809CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17921positivegrowth28mesophilic
30482positivegrowth25-30mesophilic
30482positiveoptimum27.5mesophilic
37934positivegrowth30mesophilic
62648positivegrowth37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30482aerobe
62648microaerophile
121809obligate aerobe

spore formation

  • @ref: 30482
  • spore formation: no

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
30482370543-hydroxybutyrate+carbon source
3048230089acetate+carbon source
3048222599arabinose+carbon source
3048228757fructose+carbon source
3048228260galactose+carbon source
3048217234glucose+carbon source
3048215361pyruvate+carbon source
3048233942ribose+carbon source
3048217992sucrose+carbon source
12180917632nitrate+reduction
12180916301nitrite-reduction

metabolite production

  • @ref: 121809
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
30482catalase+1.11.1.6
30482cytochrome oxidase+1.9.3.1
121809oxidase-
121809catalase+1.11.1.6
121809urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121809-+++-+----++---++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
17921wastewater treatment bioreactorAachenGermanyDEUEurope
62648Waste water,bioreactor
67770Wastewater treatment bioreactorAechenGermanyDEUEurope
121809Environment, Wastewater bioreactorGermanyDEUEurope2009

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Waste#Wastewater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179211Risk group (German classification)
1218091Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17921
  • description: Gordonia phosphorivorans partial 16S rRNA gene, type strain Ca8T
  • accession: HE574551
  • length: 1372
  • database: ena
  • NCBI tax ID: 1056982

GC content

  • @ref: 17921
  • GC-content: 67

External links

@ref: 17921

culture collection no.: DSM 45630, CCM 7957, CCUG 61533, LMG 26648, JCM 30490, CIP 110338

straininfo link

  • @ref: 79896
  • straininfo: 378857

literature

  • topic: Phylogeny
  • Pubmed-ID: 22389287
  • title: Gordonia phosphorivorans sp. nov., isolated from a wastewater bioreactor with phosphorus removal.
  • authors: Kampfer P, Martin K, Dott W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.037093-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Gordonia Bacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phosphorus/*metabolism, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis, Waste Water/microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17921Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45630)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45630
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30482Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2681628776041
37934Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8186
62648Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61533)https://www.ccug.se/strain?id=61533
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
79896Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID378857.1StrainInfo: A central database for resolving microbial strain identifiers
121809Curators of the CIPCollection of Institut Pasteur (CIP 110338)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110338