Strain identifier

BacDive ID: 10521

Type strain: No

Species: Aquaspirillum dispar

Strain Designation: 104

Strain history: CIP <- 2001, CCUG, Aquaspirillum dispar <- 1983, DSMZ <- M. Aragno <- NR. Krieg <- HW. Jannasch

NCBI tax ID(s): 57480 (species)

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General

@ref: 380

BacDive-ID: 10521

DSM-Number: 736

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Aquaspirillum dispar 104 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from fresh water.

NCBI tax id

  • NCBI tax id: 57480
  • Matching level: species

strain history

@refhistory
380<- M. Aragno <- N.R. Krieg <- H.W. Jannasch, 104
67770IAM 14943 <-- IFO 15328 <-- LMG 4329 <-- N. Krieg VPI 1.
116064CIP <- 2001, CCUG, Aquaspirillum dispar <- 1983, DSMZ <- M. Aragno <- NR. Krieg <- HW. Jannasch

doi: 10.13145/bacdive10521.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Chromobacteriaceae
  • genus: Aquaspirillum
  • species: Aquaspirillum dispar
  • full scientific name: Aquaspirillum dispar Hylemon et al. 1973 (Approved Lists 1980)

@ref: 380

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Chromobacteriaceae

genus: Microvirgula

species: Microvirgula aerodenitrificans

full scientific name: Microvirgula aerodenitrificans Patureau et al. 1998 emend. Cleenwerck et al. 2003

strain designation: 104

type strain: no

Morphology

cell morphology

  • @ref: 116064
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
380SPIRILLUM MEDIUM (DSMZ Medium 37)yeshttps://mediadive.dsmz.de/medium/37Name: SPIRILLUM MEDIUM (DSMZ Medium 37) Composition: Peptone 10.0 g/l (NH4)2SO4 1.0 g/l Succinic acid 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled water
33742MEDIUM 65 - for Prolinoborus fasciculusyesDistilled water make up to (1000.000 ml);Manganese II sulphate monohydrate(2.000 mg);Magnesium sulphate heptahydrate (1.000 g);Agar (15.000 g);Ammonium sulphate (1.000 g);Peptone (10.000 g);Succinic acid (1.680 g);Ferric III chloride hexahydrate (2.000mg)
116064CIP Medium 65yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=65

culture temp

@refgrowthtypetemperaturerange
380positivegrowth30mesophilic
33742positivegrowth30mesophilic
46012positivegrowth30mesophilic
67770positivegrowth30mesophilic
116064positivegrowth15-41
116064nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46012aerobe
116064obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
116064NaClpositivegrowth0-2 %
116064NaClnogrowth4 %
116064NaClnogrowth6 %
116064NaClnogrowth8 %
116064NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11606416947citrate+carbon source
1160644853esculin-hydrolysis
11606417234glucose-fermentation
11606417716lactose-fermentation
11606417632nitrate+reduction
11606416301nitrite+reduction
116064132112sodium thiosulfate-builds gas from
11606417632nitrate+respiration

antibiotic resistance

  • @ref: 116064
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116064
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11606415688acetoin-
11606417234glucose-

enzymes

@refvalueactivityec
116064oxidase+
116064beta-galactosidase-3.2.1.23
116064alcohol dehydrogenase+1.1.1.1
116064gelatinase-
116064amylase-
116064caseinase-3.4.21.50
116064catalase+1.11.1.6
116064tween esterase-
116064gamma-glutamyltransferase-2.3.2.2
116064lecithinase-
116064lipase-
116064lysine decarboxylase-4.1.1.18
116064ornithine decarboxylase-4.1.1.17
116064phenylalanine ammonia-lyase-4.3.1.24
116064tryptophan deaminase-
116064urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116064-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116064-------------------------------------------------++--+--------+--+------++-+++-+++-+--+-++++++-++--

Isolation, sampling and environmental information

isolation

@refsample type
380fresh water
46012Fresh water
67770Freshwater
116064Environment, Fresh water

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_5074.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_2474;97_3019;98_3787;99_5074&stattab=map
  • Last taxonomy: Microvirgula
  • 16S sequence: AB074526
  • Sequence Identity:
  • Total samples: 4775
  • soil counts: 416
  • aquatic counts: 2210
  • animal counts: 1828
  • plant counts: 321

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
3801Risk group (German classification)
1160641Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Aquaspirillum dispar gene for 16S rRNA, partial sequence
  • accession: AB074526
  • length: 1503
  • database: ena
  • NCBI tax ID: 57480

GC content

  • @ref: 380
  • GC-content: 65.0

External links

@ref: 380

culture collection no.: DSM 736, ATCC 27510, CCUG 13793, CIP 107296, JCM 21425, IAM 14943, IFO 15328, LMG 4329, NBRC 15328, VKM B-1401

straininfo link

  • @ref: 79797
  • straininfo: 893

literature

  • topic: Genetics
  • Pubmed-ID: 13130033
  • title: Aquaspirillum dispar Hylemon et al. 1973 and Microvirgula aerodenitrificans Patureau et al. 1998 are subjective synonyms.
  • authors: Cleenwerck I, De Wachter M, Hoste B, Janssens D, Swings J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02675-0
  • year: 2003
  • mesh: DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Neisseriaceae/*classification/genetics/metabolism, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
380Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 736)https://www.dsmz.de/collection/catalogue/details/culture/DSM-736
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33742Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4715
46012Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 13793)https://www.ccug.se/strain?id=13793
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
79797Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID893.1StrainInfo: A central database for resolving microbial strain identifiers
116064Curators of the CIPCollection of Institut Pasteur (CIP 107296)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107296