Strain identifier

BacDive ID: 10493

Type strain: Yes

Species: Neisseria animaloris

Strain Designation: CL 191/78, Vandamme R-24680, D 8251

Strain history: CIP <- 1990, ATCC <- CDC: strain D 8251 <- Wisconsin State Lab. Hyg., USA <- C.H. Altshuler

NCBI tax ID(s): 326522 (species)

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General

@ref: 15856

BacDive-ID: 10493

DSM-Number: 21642

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Neisseria animaloris CL 191/78 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from thumb wound.

NCBI tax id

  • NCBI tax id: 326522
  • Matching level: species

strain history

@refhistory
15856<- LMG {2008} <- P. Vandamme, Univ. Ghent, Belgium; Vandamme R-24680 (CDC group EF-4a) <- NCTC <- B. Holmes <- CDC, Atlanta, USA <- Wisconsin State Lab. Hyg. <- C.H. Altshuler
116394CIP <- 1990, ATCC <- CDC: strain D 8251 <- Wisconsin State Lab. Hyg., USA <- C.H. Altshuler

doi: 10.13145/bacdive10493.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria animaloris
  • full scientific name: Neisseria animaloris Vandamme et al. 2006

@ref: 15856

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria animaloris

full scientific name: Neisseria animaloris Vandamme et al. 2006

strain designation: CL 191/78, Vandamme R-24680, D 8251

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31732negativerod-shapedno
69480negative99.99
116394negativerod-shapedno

pigmentation

  • @ref: 31732
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15856COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
34264MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116394CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116394CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
15856positivegrowth37mesophilic
31732positivegrowth18-37
31732positiveoptimum18-22psychrophilic
34264positivegrowth30mesophilic
116394positivegrowth25-41
116394nogrowth15psychrophilic
116394nogrowth45thermophilic

culture pH

@refabilitytypepH
31732positivegrowth7
31732positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31732facultative anaerobe
116394obligate aerobe

spore formation

@refspore formationconfidence
31732no
69481no100
69480no99.998

observation

  • @ref: 31732
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3173216947citrate+carbon source
3173217632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
11639416947citrate-carbon source
1163944853esculin-hydrolysis
11639417234glucose+fermentation
116394606565hippurate+hydrolysis
11639417716lactose-fermentation
11639417632nitrate+reduction
11639416301nitrite+reduction
116394132112sodium thiosulfate-builds gas from
11639417234glucose+degradation
11639417632nitrate+respiration
6837717634D-glucose+builds acid from
6837717306maltose-builds acid from
6837716199urea-hydrolysis

antibiotic resistance

  • @ref: 116394
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11639435581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11639415688acetoin-
11639417234glucose+

enzymes

@refvalueactivityec
31732catalase+1.11.1.6
31732gelatinase+
31732cytochrome oxidase+1.9.3.1
68377proline-arylamidase+3.4.11.5
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377beta-lactamase+3.5.2.6
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
116394oxidase+
116394beta-galactosidase-3.2.1.23
116394alcohol dehydrogenase+1.1.1.1
116394gelatinase-
116394catalase+1.11.1.6
116394gamma-glutamyltransferase+2.3.2.2
116394lysine decarboxylase-4.1.1.18
116394ornithine decarboxylase-4.1.1.17
116394phenylalanine ammonia-lyase-4.3.1.24
116394tryptophan deaminase-
116394urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116394-++--+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
15856+--+--+-+-----+/---+/---+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116394----+-----+/---------------------------------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
15856++--+----++-+
15856+++--+----++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116394+------------------+----------------------------++-------------------------+---++---------+---+---+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15856thumb woundWisconsinUSAUSANorth America
116394Human, Dog biteWisconsinUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Wound
#Host Body-Site#Limb#Hand

taxonmaps

  • @ref: 69479
  • File name: preview.99_4911.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_837;97_979;98_3677;99_4911&stattab=map
  • Last taxonomy: Neisseria animaloris subclade
  • 16S sequence: DQ006842
  • Sequence Identity:
  • Total samples: 1076
  • soil counts: 24
  • aquatic counts: 73
  • animal counts: 977
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
158562Risk group (German classification)
1163941Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15856
  • description: Neisseria animaloris strain LMG 23011 16S ribosomal RNA gene, partial sequence
  • accession: DQ006842
  • length: 1407
  • database: ena
  • NCBI tax ID: 326522

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria animaloris NCTC12228GCA_900637855completencbi326522
66792Neisseria animaloris DSM 21642GCA_002108605contigncbi326522
66792Neisseria animaloris strain DSM 21642326522.3wgspatric326522
66792Neisseria animaloris strain NCTC12228326522.7completepatric326522

GC content

@refGC-contentmethod
1585649.3high performance liquid chromatography (HPLC)
3173249.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno95.109yes
gram-positiveno98.411yes
anaerobicno98.731no
aerobicno91.002yes
halophileno69.499no
spore-formingno96.39no
thermophileno99.079yes
glucose-utilyes73.492no
motileno86.783yes
glucose-fermentno89.138yes

External links

@ref: 15856

culture collection no.: DSM 21642, ATCC 29858, CDC D8251, LMG 23011, NCTC 12228, CIP 103440

straininfo link

  • @ref: 79770
  • straininfo: 39764

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16902011Classification of Centers for Disease Control Group Eugonic Fermenter (EF)-4a and EF-4b as Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov., respectively.Vandamme P, Holmes B, Bercovier H, Coenye TInt J Syst Evol Microbiol10.1099/ijs.0.64142-02006Animals, Bites and Stings, DNA, Bacterial/genetics, Dogs, Humans, Molecular Sequence Data, Neisseria/*classification/genetics/metabolism, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Wounds and Injuries/microbiologyPathogenicity
Phylogeny34797758Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta).Busse HJ, Kampfer P, Szostak MP, Ruckert C, Spergser JInt J Syst Evol Microbiol10.1099/ijsem.0.0051142021Animals, Bacterial Typing Techniques, Base Composition, Cloaca/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Neisseriaceae/*classification/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Turtles/microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15856Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21642)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21642
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31732Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2800828776041
34264Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15238
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
79770Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39764.1StrainInfo: A central database for resolving microbial strain identifiers
116394Curators of the CIPCollection of Institut Pasteur (CIP 103440)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103440