Strain identifier

BacDive ID: 10485

Type strain: Yes

Species: Neisseria canis

Strain Designation: H6

Strain history: CIP <- 1989, NCTC <- 1962, U. Berger: strain H6

NCBI tax ID(s): 493 (species)

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General

@ref: 7227

BacDive-ID: 10485

DSM-Number: 18000

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, human pathogen

description: Neisseria canis H6 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from oral mucosa of dog.

NCBI tax id

  • NCBI tax id: 493
  • Matching level: species

strain history

@refhistory
7227<- CIP <- NCTC <- U. Berger, Hamburg; H6
117139CIP <- 1989, NCTC <- 1962, U. Berger: strain H6

doi: 10.13145/bacdive10485.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria canis
  • full scientific name: Neisseria canis Berger 1962 (Approved Lists 1980)

@ref: 7227

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria canis

full scientific name: Neisseria canis Berger 1962

strain designation: H6

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.996
117139negativecoccus-shapedno

colony morphology

  • @ref: 117139

multimedia

  • @ref: 7227
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_18000.jpg
  • caption: Neisseria canis showing the typical yellowish colour when cultivated on Columbia Agar + 5% sheep blood
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7227BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
7227COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
40604MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
117139CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
7227positivegrowth37mesophilic
40604positivegrowth37mesophilic
61085positivegrowth37mesophilic
117139positivegrowth25-41

Physiology and metabolism

oxygen tolerance

  • @ref: 61085
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11713915824D-fructose-degradation
11713917634D-glucose-degradation
11713916024D-mannose-degradation
11713917716lactose-degradation
11713917306maltose-degradation
11713917992sucrose-degradation
117139606565hippurate+hydrolysis
11713917632nitrate-builds gas from
11713917632nitrate+reduction
11713916301nitrite-builds gas from
11713916301nitrite-reduction
11713917632nitrate-respiration
6837717634D-glucose+builds acid from
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11713935581indoleno
117139polysaccharidesno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68377tryptophan deaminase-4.1.99.1
68377proline-arylamidase+3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
117139oxidase+
117139beta-galactosidase-3.2.1.23
117139alcohol dehydrogenase-1.1.1.1
117139gelatinase-
117139amylase-
117139DNase-
117139caseinase-3.4.21.50
117139catalase+1.11.1.6
117139tween esterase-
117139gamma-glutamyltransferase-2.3.2.2
117139lecithinase-
117139lipase-
117139lysine decarboxylase-4.1.1.18
117139ornithine decarboxylase-4.1.1.17
117139protease-
117139urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
44499-+++-+--------------
117139-+++-+----++---+----

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
7227-+--------++/--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117139---------------------------------------------------------------------------+--------------+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
7227oral mucosa of dogHamburgGermanyDEUEurope
44499Dog,oral mucosaGermanyDEUEurope
61085Dog oral mucosaHamburgGermanyDEUEurope1962
117139Animal, Dog, oral mucosaHamburgGermanyDEUEurope1962

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Oral cavity and airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_796.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_484;97_553;98_636;99_796&stattab=map
  • Last taxonomy: Levilactobacillus
  • 16S sequence: EF120367
  • Sequence Identity:
  • Total samples: 29434
  • soil counts: 2112
  • aquatic counts: 2988
  • animal counts: 22485
  • plant counts: 1849

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7227yes, in single cases1Risk group (German classification)
1171391Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neisseria canis 16S rRNA gene, partialAJ247235457ena493
20218Lactobacillus brevis strain ATCC 14687 16S ribosomal RNA gene, partial sequenceEF1203671551ena1580
20218Moraxella canis strain CCUG 2153 16S-23S ribosomal RNA intergenic spacer, complete sequenceEU014607625ena90239
7227Neisseria canis strain ATCC 14687 16S ribosomal RNA gene, partial sequenceL061701487ena493

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria canis NCTC10296GCA_900636765completencbi493
66792Neisseria canis ATCC 14687GCA_002108495contigncbi493
66792Neisseria canis strain ATCC 14687493.4wgspatric493
66792Neisseria canis strain NCTC10296493.7completepatric493
66792Neisseria canis NCTC102962877225389completeimg493

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno96.108no
gram-positiveno98.372no
anaerobicno98.821no
aerobicno80.936yes
halophileno74.575no
spore-formingno96.904no
thermophileno98.806no
glucose-utilyes62.886no
motileno91.776no
glucose-fermentno91.132no

External links

@ref: 7227

culture collection no.: DSM 18000, ATCC 14687, CCUG 2153, CIP 103347, LMG 8383, NCTC 10296, CCUG 56775

straininfo link

  • @ref: 79762
  • straininfo: 3628

literature

  • topic: Phylogeny
  • Pubmed-ID: 30798492
  • title: Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae).
  • authors: Zhang G, Yang J, Lai XH, Lu S, Jin D, Pu J, Bai X, Luo X, Xiong Y, Huang Y, Chen C, Xu J
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-019-01234-2
  • year: 2019
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Lagomorpha/*microbiology, Neisseria/classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Rectum/*microbiology, Tibet
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7227Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18000)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18000
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40604Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15135
44499Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2153)https://www.ccug.se/strain?id=2153
61085Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56775)https://www.ccug.se/strain?id=56775
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
79762Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3628.1StrainInfo: A central database for resolving microbial strain identifiers
117139Curators of the CIPCollection of Institut Pasteur (CIP 103347)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103347