Strain identifier

BacDive ID: 10466

Type strain: Yes

Species: Neisseria cinerea

Strain Designation: 194

Strain history: CIP <- 1973, ATCC <- U. Berger: strain 194

NCBI tax ID(s): 546262 (strain), 483 (species)

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General

@ref: 1806

BacDive-ID: 10466

DSM-Number: 4630

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, coccus-shaped, human pathogen

description: Neisseria cinerea 194 is an obligate aerobe, Gram-negative, coccus-shaped human pathogen that was isolated from Nasopharynx.

NCBI tax id

NCBI tax idMatching level
546262strain
483species

strain history

@refhistory
1806<- NCTC <- U. Berger
118286CIP <- 1973, ATCC <- U. Berger: strain 194

doi: 10.13145/bacdive10466.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria cinerea
  • full scientific name: Neisseria cinerea (von Lingelsheim 1906) Murray 1939 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Micrococcus cinereus

@ref: 1806

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria cinerea

full scientific name: Neisseria cinerea (von Lingelsheim 1906) Murray 1939

strain designation: 194

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
118286negativecoccus-shapedno
69480negative100

colony morphology

@reftype of hemolysisincubation period
1806gamma1-2 days
118286

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1806COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
42051MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
118286CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperature
1806positivegrowth30
42051positivegrowth37
118286positivegrowth25-41

Physiology and metabolism

oxygen tolerance

  • @ref: 118286
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69480no90.67
69481no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11828615824D-fructose-degradation
11828617634D-glucose-degradation
11828617716lactose-degradation
11828617306maltose-degradation
11828617992sucrose-degradation
11828617632nitrate-builds gas from
11828617632nitrate-reduction
11828616301nitrite-builds gas from
11828616301nitrite+reduction
11828617632nitrate-respiration
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11828635581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
1806catalase+1.11.1.6
1806cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
118286oxidase+
118286beta-galactosidase-3.2.1.23
118286alcohol dehydrogenase-1.1.1.1
118286gelatinase-
118286amylase-
118286DNase-
118286caseinase-3.4.21.50
118286catalase+1.11.1.6
118286tween esterase-
118286gamma-glutamyltransferase-2.3.2.2
118286lecithinase-
118286lipase-
118286lysine decarboxylase-4.1.1.18
118286ornithine decarboxylase-4.1.1.17
118286urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118286--++-+----++---+----

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
1806+/---------+---
1806---------+---
1806+--------+---
1806----------+--
1806-+--------+--
1806-+/---------+--
1806----------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
1806NasopharynxHamburgGermanyDEUEurope
118286Human, Normal mucosa of nasopharynxHamburgGermanyDEUEurope1962

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways
  • Cat3: #Throat

taxonmaps

  • @ref: 69479
  • File name: preview.99_777.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_42;97_98;98_107;99_777&stattab=map
  • Last taxonomy: Neisseria
  • 16S sequence: AB904035
  • Sequence Identity:
  • Total samples: 903
  • soil counts: 4
  • aquatic counts: 7
  • animal counts: 891
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
1806yes, in single cases1Risk group (German classification)
1182861Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neisseria cinerea gene for 16S ribosomal RNA, partial sequence, strain: NCI_ATCC14685AB9040351085nuccore483
20218Neisseria cinerea 16S rRNA gene, partialAJ247236457nuccore483
124043Neisseria cinerea ATCC 14685 gene for 16S rRNA, partial sequence.LC752458588nuccore483

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria cinerea NCTC10294GCA_900475315completencbi483
66792Neisseria cinerea ATCC 14685GCA_000173895contigncbi546262
66792Neisseria cinerea ATCC 14685546262.4wgspatric546262
66792Neisseria cinerea strain FDAARGOS_871483.52completepatric483
66792Neisseria cinerea strain NCTC10294483.11completepatric483
66792Neisseria cinerea ATCC 146852562617169draftimg546262
66792Neisseria cinerea NCTC 102942814123399completeimg483

GC content

  • @ref: 1806
  • GC-content: 49.0-50.9

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingno100no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no94.678yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes54.594yes
69480spore-formingspore-formingAbility to form endo- or exosporesno90.67no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.982yes
69480flagellatedmotile2+Ability to perform flagellated movementno87.421no

External links

@ref: 1806

culture collection no.: DSM 4630, ATCC 14685, NCTC 10294, CIP 73.16, CCUG 2156, LMG 8380

straininfo link

  • @ref: 79743
  • straininfo: 55641

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1806Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4630)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4630
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42051Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10669
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
79743Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID55641.1StrainInfo: A central database for resolving microbial strain identifiers
118286Curators of the CIPCollection of Institut Pasteur (CIP 73.16)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.16
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy