Strain identifier

BacDive ID: 10437

Type strain: Yes

Species: Aquaspirillum arcticum

Strain Designation: Res-10

Strain history: CIP <- 2001, NCIMB <- W.E. Inniss: strain Res-10

NCBI tax ID(s): 87645 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2669

BacDive-ID: 10437

DSM-Number: 6444

keywords: 16S sequence, Bacteria, psychrophilic, Gram-negative, motile, rod-shaped

description: Aquaspirillum arcticum Res-10 is a psychrophilic, Gram-negative, motile bacterium that was isolated from sediment under snow and ice.

NCBI tax id

  • NCBI tax id: 87645
  • Matching level: species

strain history

@refhistory
2669<- ATCC <- W.E. Inniss, Res-10
67770IAM 14963 <-- ATCC 49402 <-- W. E. Inniss Res-10.
116476CIP <- 2001, NCIMB <- W.E. Inniss: strain Res-10

doi: 10.13145/bacdive10437.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Chromobacteriaceae
  • genus: Aquaspirillum
  • species: Aquaspirillum arcticum
  • full scientific name: Aquaspirillum arcticum Butler et al. 1990

@ref: 2669

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Chromobacteriaceae

genus: Aquaspirillum

species: Aquaspirillum arcticum

full scientific name: Aquaspirillum arcticum Butler et al. 1990

strain designation: Res-10

type strain: yes

Morphology

cell morphology

  • @ref: 116476
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2669TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
2669HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)yeshttps://mediadive.dsmz.de/medium/428Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
36602MEDIUM 14 - Williams and Rittenberg medium for Aquaspirillum genusyesDistilled water make up to (1000.000 ml);Agar (13.000 g);Yeast extract (3.000 g);Peptone (5.000 g);Beef extract (3.000 g)
116476CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116476CIP Medium 14yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=14

culture temp

@refgrowthtypetemperaturerange
2669positivegrowth4psychrophilic
36602positivegrowth6psychrophilic
67770positivegrowth5psychrophilic
116476positivegrowth5-15psychrophilic
116476nogrowth22psychrophilic
116476nogrowth30mesophilic
116476nogrowth37mesophilic
116476nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
116476NaClpositivegrowth0 %
116476NaClnogrowth2 %
116476NaClnogrowth4 %
116476NaClnogrowth6 %
116476NaClnogrowth8 %
116476NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11647616947citrate-carbon source
1164764853esculin-hydrolysis
11647617632nitrate-reduction
11647616301nitrite-reduction

antibiotic resistance

  • @ref: 116476
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116476
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11647615688acetoin-
11647617234glucose-

enzymes

@refvalueactivityec
116476oxidase+
116476beta-galactosidase-3.2.1.23
116476alcohol dehydrogenase-1.1.1.1
116476gelatinase-
116476amylase-
116476DNase-
116476caseinase-3.4.21.50
116476catalase+1.11.1.6
116476tween esterase-
116476gamma-glutamyltransferase-2.3.2.2
116476lecithinase-
116476lipase-
116476lysine decarboxylase-4.1.1.18
116476ornithine decarboxylase-4.1.1.17
116476phenylalanine ammonia-lyase-4.3.1.24
116476protease-
116476tryptophan deaminase-
116476urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116476-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116476---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2669sediment under snow and iceNorthwest Territory, ResoluteCanadaCANNorth America
67770Sediment under snow and iceResolute, Northwest TerritoryCanadaCANNorth America
116476Environment, Sediment, snow and iceNorthwest TerritoryCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Ice
#Environmental#Aquatic#Sediment

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
26691Risk group (German classification)
1164761Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Aquaspirillum arcticum gene for 16S rRNA, partial sequence
  • accession: AB074523
  • length: 1498
  • database: ena
  • NCBI tax ID: 87645

External links

@ref: 2669

culture collection no.: DSM 6444, ATCC 49402, JCM 21435, IAM 14963, CIP 107313, NCIMB 13152

straininfo link

  • @ref: 79718
  • straininfo: 42453

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2669Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6444)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6444
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36602Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4735
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
79718Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42453.1StrainInfo: A central database for resolving microbial strain identifiers
116476Curators of the CIPCollection of Institut Pasteur (CIP 107313)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107313