Archive BacDiveID:10.13145/bacdive10429.20230509.8.1

Strain identifier

BacDive ID: 10429

Type strain:

Species: Nautilia lithotrophica

Strain Designation: 525

Strain history: DSM 13520 <-- M. L. Miroshnichenko 525.

NCBI tax ID(s): 128945 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5072

BacDive-ID: 10429

DSM-Number: 13520

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Nautilia lithotrophica 525 is an anaerobe, thermophilic bacterium that was isolated from Alvinella pompejana .

NCBI tax id

NCBI tax id: 128945
Matching level: species

strain history

@ref	history
5072	<- M. L. Miroshnichenko, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; 525 <- M. L. Miroshnichenko {2001}
67770	DSM 13520 <-- M. L. Miroshnichenko 525.

doi: 10.13145/bacdive10429.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Epsilonproteobacteria
order: Nautiliales
family: Nautiliaceae
genus: Nautilia
species: Nautilia lithotrophica
full scientific name: Nautilia lithotrophica Miroshnichenko et al. 2002

@ref: 5072

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Nautiliales

family: Nautiliaceae

genus: Nautilia

species: Nautilia lithotrophica

full scientific name: Nautilia lithotrophica Miroshnichenko et al. 2002

strain designation: 525

type strain: yes

Culture and growth conditions

culture medium

@ref: 5072
name: NAUTILIA MEDIUM (DSMZ Medium 946)
growth: yes
link: https://mediadive.dsmz.de/medium/946
composition: Name: NAUTILIA MEDIUM (DSMZ Medium 946) Composition: Sulfur 9.8912 g/l MgCl2 x 6 H2O 5.44016 g/l Na-formate 2.96736 g/l Na2CO3 1.48368 g/l KCl 0.642928 g/l Na2S x 9 H2O 0.593472 g/l NH4Cl 0.326409 g/l KH2PO4 0.326409 g/l NaBr 0.098912 g/l MgSO4 x 7 H2O 0.0296736 g/l SrCl2 x 6 H2O 0.0148368 g/l Nitrilotriacetic acid 0.0148368 g/l Na3-citrate 0.0098912 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l Pyridoxine hydrochloride 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l KI 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
5072	positive	growth	55	thermophilic
67770	positive	growth	55	thermophilic

Physiology and metabolism

oxygen tolerance

@ref: 5072
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	host species	geographic location	country	latitude	longitude
5072	Alvinella pompejana (a deep-sea hydrothermal vent polychaete)	Alvinella pompejana	East Pacific Rise	International waters	12.8	-103.933
67770	Tubes of the deep-sea hydrothermal vent polychaete Alvinella pompejana	Alvinella pompejana	East Pacific Rise	Pacific Ocean

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Hydrothermal vent
#Host	#Invertebrates (Other)	#Annelida
#Condition	#Thermophilic (>45°C)

Safety information

risk assessment

@ref: 5072
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 5072
description: epsilon proteobacterium 525 16S rRNA gene
accession: AJ404370
length: 1464
database: ena
NCBI tax ID: 128945

GC content

@ref: 5072
GC-content: 34.7
method: high performance liquid chromatography (HPLC)

External links

@ref: 5072

culture collection no.: DSM 13520, JCM 15786

straininfo link

@ref: 79711
straininfo: 100804

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	12148643	Nautilia lithotrophica gen. nov., sp. nov., a thermophilic sulfur-reducing epsilon-proteobacterium isolated from a deep-sea hydrothermal vent.	Miroshnichenko ML, Kostrikina NA, L'Haridon S, Jeanthon C, Hippe H, Stackebrandt E, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1299	2002	Animals, DNA, Ribosomal/analysis, Epsilonproteobacteria/classification/growth & development/isolation & purification/ultrastructure, Hot Temperature, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, Polychaeta/microbiology, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, Sulfur/*metabolism	Stress
Phylogeny	18599701	Nautilia profundicola sp. nov., a thermophilic, sulfur-reducing epsilonproteobacterium from deep-sea hydrothermal vents.	Smith JL, Campbell BJ, Hanson TE, Zhang CL, Cary SC	Int J Syst Evol Microbiol	10.1099/ijs.0.65435-0	2008	Base Composition, DNA, Bacterial/chemistry, Epsilonproteobacteria/chemistry/classification/genetics/physiology, Fatty Acids/metabolism, Formates/metabolism, Molecular Sequence Data, Oceans and Seas, RNA, Ribosomal, 16S/genetics, Species Specificity, Sulfur/metabolism, Temperature, Water Microbiology	Metabolism

Reference

@id	authors	catalogue	doi/url	title
5072	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13520)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-13520
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
79711	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID100804.1	StrainInfo: A central database for resolving microbial strain identifiers