Strain identifier

BacDive ID: 1006

Type strain: Yes

Species: Bacillus thuringiensis

Strain Designation: NRS-996

Strain history: CIP <- 1953, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain NRS-996 <- J.R. Porter <- O. Mattes

NCBI tax ID(s): 1428 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 688

BacDive-ID: 1006

DSM-Number: 2046

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Bacillus thuringiensis NRS-996 is a facultative anaerobe, spore-forming, mesophilic human pathogen that was isolated from mediterranean flour moth.

NCBI tax id

  • NCBI tax id: 1428
  • Matching level: species

strain history

@refhistory
688<- ATCC <- E.A. Steinhaus <- O. Mattes
67770IAM 12077 <-- ATCC 10792 <-- E. A. Steinhaus <-- O. Mattes.
123541CIP <- 1953, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain NRS-996 <- J.R. Porter <- O. Mattes

doi: 10.13145/bacdive1006.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus thuringiensis
  • full scientific name: Bacillus thuringiensis Berliner 1915 (Approved Lists 1980)

@ref: 688

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus thuringiensis

full scientific name: Bacillus thuringiensis Berliner 1915

strain designation: NRS-996

type strain: yes

Morphology

cell morphology

  • @ref: 123541
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123541
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
688NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36265MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123541CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123541CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
688positivegrowth30mesophilic
36265positivegrowth30mesophilic
67770positivegrowth30mesophilic
123541positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123541
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 123541
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12354117632nitrate+reduction
12354116301nitrite-reduction
12354117632nitrate+respiration
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen+fermentation

metabolite production

  • @ref: 123541
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
123541oxidase-
123541alcohol dehydrogenase+1.1.1.1
123541catalase+1.11.1.6
123541lysine decarboxylase-4.1.1.18
123541ornithine decarboxylase-4.1.1.17
123541urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
44998++++---++-+-+--+-+++

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123541-+++-+---+++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123541++-++--+-++----+++++--+--------+---+-------------++--+-----++--------------+----+-----+-+++---+-+-+

Isolation, sampling and environmental information

isolation

@refsample typehost species
688mediterranean flour moth
44998Mediterranean flour moth (Ephestia kuhniella)
67770Mediterranean flour moth (Ephestia kuehniella)Ephestia kuehniella
123541Mediterranean flour moth

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

taxonmaps

  • @ref: 69479
  • File name: preview.99_26.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_21;97_22;98_25;99_26&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: D16281
  • Sequence Identity:
  • Total samples: 4969
  • soil counts: 1920
  • aquatic counts: 446
  • animal counts: 1524
  • plant counts: 1079

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
688yes, in single casesyes1Risk group (German classification)
1235411Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus thuringiensis strain ATCC10792 16S ribosomal RNA gene, partial sequenceAF2905451482ena1428
20218Bacillus thuringiensis strain ATCC 10792 16S ribosomal RNA gene, partial sequenceGQ911556806ena1428
20218Bacillus thuringiensis partial 16S rRNA gene and ITS1, strain DSM 2046AJ841875542ena1428
20218B.thuringiensis DNA for 16S and 23S rRNA and spacer regionX898952978ena1428
20218Bacillus thuringiensis gene for 16S rRNA, partial sequenceAB006940277ena1428
20218Bacillus thuringiensis gene for 16S rRNA, partial sequence, strain: GTC 02829AB5925401363ena1428
20218Bacillus thuringiensis gene for 16S rRNA, partial sequence, strain: IAM 12077D162811486ena1428
20218Bacillus thuringiensis gene for 16S ribosomal RNA, partial sequence, strain: NBRC 101235AB4264791477ena1428
20218B.thuringensis 16S ribosomal RNAX550621450ena1428

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus thuringiensis serovar berliner ATCC 10792GCA_000161615chromosomencbi527031
66792Bacillus thuringiensis ATCC 10792GCA_002119445completencbi1428
66792Bacillus thuringiensis ATCC 10792GCA_002243685completencbi1428
66792Bacillus thuringiensis serovar berliner ATCC 10792527031.3wgspatric527031
66792Bacillus thuringiensis strain ATCC 107921428.847plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.425completepatric1428
66792Bacillus thuringiensis strain ATCC 107921428.841plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.478completepatric1428
66792Bacillus thuringiensis strain ATCC 107921428.845plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.842plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.848plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.844plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.849plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.840plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.846plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.843plasmidpatric1428
66792Bacillus thuringiensis strain ATCC 107921428.850plasmidpatric1428
66792Bacillus thuringiensis sv. berliner ATCC 107922916873227completeimg527031
66792Bacillus thuringiensis sv. berliner ATCC 107922912849059completeimg527031
66792Bacillus thuringiensis sv. berliner ATCC 10792643886085draftimg527031

GC content

@refGC-contentmethod
68834.3Buoyant density centrifugation (BD)
68833.8thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes73.615no
gram-positiveyes80.961no
anaerobicno98.758no
halophileno94.339no
spore-formingyes91.735no
glucose-utilyes89.711no
aerobicyes79.98no
thermophileno99.836yes
motileyes60.496no
glucose-fermentno91.289no

External links

@ref: 688

culture collection no.: DSM 2046, ATCC 10792, CCM 19, NCIB 9134, CCUG 7429, JCM 20386, BCRC 14683, CCEB 457, CECT 197, CIP 53.137, CN 3624, HAMBI 252, HAMBI 478, IAM 12077, LBG B-4034, LMD 70.8, LMG 7138, NBRC 101235, NCAIM B.01292, NCCB 70008, NCDO 1146, NCFB 1146, NCIMB 9134, NCIMB 9207, NRRL HD-735, NRRL NRS-996, PCM 1853, VKM B-439, VKM B-454, VTT E-86245, WDCM 00221

straininfo link

  • @ref: 70679
  • straininfo: 389259

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1126915Production of molybdenum-coordinating compound by Bacillus thuringiensis.Ketchum PA, Owens MSJ Bacteriol10.1128/jb.122.2.412-417.19751975Amino Acids/analysis, Arginine/metabolism, Bacillus thuringiensis/*metabolism, Bacterial Proteins/analysis/*biosynthesis, Chromatography, Gel, Chromatography, Thin Layer, Culture Media, Electrophoresis, Polyacrylamide Gel, Hydroxylamines/analysis, Molecular Weight, Molybdenum/*metabolism, *Peptide Biosynthesis, Peptides/analysis, Spectrophotometry, Ultraviolet, StereoisomerismCultivation
Enzymology6885748Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis.Ikezawa H, Nakabayashi T, Suzuki K, Nakajima M, Taguchi T, Taguchi RJ Biochem10.1093/oxfordjournals.jbchem.a1343151983Bacillus thuringiensis/*enzymology, Isoelectric Point, Molecular Weight, Phosphatidylinositols, Phospholipases/*isolation & purification, Type C Phospholipases/*isolation & purificationPhylogeny
Phylogeny10103241Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice.Yamada S, Ohashi E, Agata N, Venkateswaran KAppl Environ Microbiol10.1128/AEM.65.4.1483-1490.19991999Amino Acid Sequence, Bacillus/*classification/*genetics, Bacillus cereus/genetics/*isolation & purification, Bacterial Typing Techniques, Base Sequence, Cloning, Molecular, DNA Gyrase, DNA Topoisomerases, Type II/*genetics, DNA, Ribosomal, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Alignment, Sequence Analysis, DNAEnzymology
Metabolism19824399Optimization of process parameters for maximum poly(-beta-)hydroxybutyrate (PHB) production by Bacillus thuringiensis IAM 12077.Pal A, Prabhu A, Kumar AA, Rajagopal B, Dadhe K, Ponnamma V, Shivakumar SPol J Microbiol2009Bacillus thuringiensis/classification/drug effects/*metabolism, Bacteriological Techniques, Bioreactors, Carbon/chemistry/pharmacology, Culture Media/chemistry, Fermentation, Hydroxybutyrates/*metabolism, Nitrogen/chemistry/pharmacology, Polyesters/*metabolism, Time FactorsBiotechnology
Phylogeny23846554New and old microbial communities colonizing a seventeenth-century wooden church.Lupan I, Ianc MB, Kelemen BS, Carpa R, Rosca-Casian O, Chiriac MT, Popescu OFolia Microbiol (Praha)10.1007/s12223-013-0265-32013Bacteria/*classification/*genetics/isolation & purification, *Biota, DNA, Bacterial/chemistry/genetics, DNA, Fungal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fungi/*classification/*genetics/isolation & purification, Humans, Molecular Sequence Data, Romania, Sequence Analysis, DNA, Wood/*microbiologyEnzymology
Metabolism24695235In vitro quenching of fish pathogen Edwardsiella tarda AHL production using marine bacterium Tenacibaculum sp. strain 20J cell extracts.Romero M, Muras A, Mayer C, Bujan N, Magarinos B, Otero ADis Aquat Organ10.3354/dao026972014Acyl-Butyrolactones/*metabolism, Bacterial Proteins/*metabolism, Edwardsiella tarda/*metabolism, Gene Expression Regulation, Bacterial/drug effects, Quorum Sensing/*drug effects, Tenacibaculum/*chemistry/classificationPathogenicity
27303383Replicates, Read Numbers, and Other Important Experimental Design Considerations for Microbial RNA-seq Identified Using Bacillus thuringiensis Datasets.Manga P, Klingeman DM, Lu TY, Mehlhorn TL, Pelletier DA, Hauser LJ, Wilson CM, Brown SDFront Microbiol10.3389/fmicb.2016.007942016
Metabolism29771325Native bacteria promote plant growth under drought stress condition without impacting the rhizomicrobiome.Armada E, Leite MFA, Medina A, Azcon R, Kuramae EEFEMS Microbiol Ecol10.1093/femsec/fiy0922018Asteraceae/*growth & development/microbiology, Bacillus thuringiensis/*metabolism, Biomass, Droughts, Ecosystem, Fungi/*metabolism, Lavandula/growth & development/*microbiology, Mycorrhizae/*growth & development, Plant Development/physiology, Plant Roots/*growth & development/microbiology, Plants/microbiology, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 18S/genetics, Rhizosphere, Soil, Soil Microbiology, Thymus Plant/*growth & development/microbiologyStress
Metabolism30326263New perspectives on Mega plasmid sequence (poh1) in Bacillus thuringiensis ATCC 10792 harbouring antimicrobial, insecticidal and antibiotic resistance genes.Chelliah R, Wei S, Park BJ, Park JH, Park YS, Kim SH, Jin YG, Oh DHMicrob Pathog10.1016/j.micpath.2018.10.0132018Animals, Anti-Bacterial Agents/*metabolism, Anti-Infective Agents/*metabolism/pharmacology, Bacillus thuringiensis/*genetics/*metabolism, Bacteria/drug effects, Bacterial Toxins/genetics, Bacteriocins/genetics/metabolism, Base Sequence, Computational Biology, DNA, Bacterial, Drug Resistance, Microbial/drug effects/*genetics, Genome, Bacterial, Insecta/drug effects, Insecticides/*metabolism/pharmacology, Microbial Sensitivity Tests, Molecular Sequence Annotation, Nisin/metabolism, Peptides/genetics/metabolism, Plasmids/*genetics, Protein Kinases/genetics, Pyrazines/metabolism, Tetracycline Resistance/genetics, Whole Genome SequencingPathogenicity
Genetics30753888Whole genome sequence of Bacillus thuringiensis ATCC 10792 and improved discrimination of Bacillus thuringiensis from Bacillus cereus group based on novel biomarkers.Chelliah R, Wei S, Park BJ, Rubab M, Banan-Mwine Dalirii E, Barathikannan K, Jin YG, Oh DHMicrob Pathog10.1016/j.micpath.2019.02.0142019Bacillus cereus/classification/*genetics, Bacillus thuringiensis/*classification/*genetics/isolation & purification, Bacterial Proteins/*genetics, Gene Expression Profiling, *Genetic Markers, *Genome, Bacterial, Molecular Sequence Annotation, Plasmids/analysis, Sequence Analysis, DNA, Vegetables/microbiology, *Whole Genome SequencingPhylogeny
Pathogenicity31046581Determination of microbiological contamination, antibacterial and antioxidant activities of natural plant hazelnut (Corylus avellana L.) pollen.Nikolaieva N, Kacaniova M, Gonzalez JC, Grygorieva O, Nozkova JJ Environ Sci Health B10.1080/03601234.2019.16037562019Anti-Bacterial Agents/*pharmacology, Antioxidants/*pharmacology, Bacteria/drug effects, Corylus/chemistry/*microbiology, Microbial Sensitivity Tests, Microbiota, Plant Extracts/chemistry/*pharmacology, Pollen/*microbiology, Slovakia, Urbanization
Pathogenicity32600016Biological properties of sea buckthorn (Hippophae rhamnoides L.) derived products.Ivanisova E, Blaskova M, Terentjeva M, Grygorieva O, Vergun O, Brindza J, Kacaniova MActa Sci Pol Technol Aliment10.17306/J.AFS.08092020Anti-Bacterial Agents/analysis/*pharmacology, Antioxidants/analysis/*pharmacology, Bacteria/drug effects, Biphenyl Compounds/metabolism, Carotenoids/analysis/*pharmacology, Chromatography, High Pressure Liquid, Flavonoids/analysis/*pharmacology, Hippophae/*chemistry, Humans, Microbial Sensitivity Tests, Molybdenum/metabolism, Phenols/analysis/pharmacology, Phytochemicals/analysis/pharmacology, Picrates/metabolism, Plant Components, Aerial/chemistry, Plant Extracts/chemistry/*pharmacology, Plant Oils/chemistry, Polyphenols/analysis/*pharmacologyMetabolism
35479535Chlorinated metabolites with antibacterial activities from a deep-sea-derived Spiromastix fungus.Niu S, Liu D, Shao Z, Huang J, Fan A, Lin WRSC Adv10.1039/d1ra05736g2021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
688Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2046)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2046
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36265Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16789
44998Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7429)https://www.ccug.se/strain?id=7429
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70679Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389259.1StrainInfo: A central database for resolving microbial strain identifiers
123541Curators of the CIPCollection of Institut Pasteur (CIP 53.137)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.137