Name: Bacillus methanolicus PB1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1231

Type strain:

Species: Bacillus methanolicus

Strain Designation: PB1

Culture col. no.: DSM 16454, ATCC 51375, LMG 24730, NCIMB 13113

Strain history: <- NCIMB; NCIMB 13113 <- L. Dijkhuizen, Univ. Groningen; PB1

NCBI tax ID(s): 1471 (species)

SI-ID 8358

LMG 16799, ATCC 51375, NCIMB 13113, KCTC 3735, LMG 21757, DSM 16454, LMG 24730

Section

Bacillus methanolicus PB1 is a thermophilic bacterium that was isolated from Waste water treatment system.

thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus methanolicus**
	Full Scientific Name (LPSN)	Bacillus methanolicus Arfman et al. 1992

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6446]

Culture medium

MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)

[Ref.: #6446]

Culture medium growth

[Ref.: #6446]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6446]

Culture medium composition

expand / minimize

[Ref.: #6446]

Culture medium

BM MEDIUM (DSMZ Medium 1192)

[Ref.: #6446]

Culture medium growth

[Ref.: #6446]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6446]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #6446]			growth	45 °C

[Ref.: #6446]

Temperature range

thermophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6446]	Sample type/isolated from	Waste water treatment system
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Wastewater
#Engineered	#Waste	#Water treatment plant

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB112727 (>99% sequence identity) for Bacillus methanolicus subclade from Microbeatlas

Aquatic Samples	2912
Soil Samples	8068
Animal Samples	5786
Plant Samples	1430
Total Samples	18196

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6446]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus methanolicus gene for 16S rRNA, partial sequence, strain:NCIMB 13113T	AB112727	1505	ENA	997296 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus methanolicus PB1	GCA_000262755	contig	GenBank	997296 tax ID	*
[Ref.: #66792]	Bacillus methanolicus PB1	997296.3	wgs	PATRIC	997296 tax ID	*
[Ref.: #66792]	Bacillus methanolicus PB1	997296.5	plasmid	PATRIC	997296 tax ID	*
[Ref.: #66792]	Bacillus methanolicus PB1	2811995044	complete	JGI IMG/M	997296 tax ID	*

[Ref.: #6446]

GC-content

48-50 mol%

Availability in culture collections External links

[Ref.: #6446]

Culture collection no.

DSM 16454, ATCC 51375, LMG 24730, NCIMB 13113

[Ref.: #70887]

SI-ID 8358

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pradoshia eiseniae gen. nov., sp. nov., a spore-forming member of the family Bacillaceae capable of assimilating 3-nitropropionic acid, isolated from the anterior gut of the earthworm Eisenia fetida.	Saha T, Ranjan VK, Ganguli S, Thakur S, Chakraborty B, Barman P, Ghosh W, Chakraborty R	Int J Syst Evol Microbiol	10.1099/ijsem.0.003304	2019	*
Phylogeny	Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China.	Zhang WY, Hu J, Zhang XQ, Zhu XF, Wu M	Antonie Van Leeuwenhoek	10.1007/s10482-015-0497-0	2015	*
Enzymology	Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties.	Krog A, Heggeset TM, Muller JE, Kupper CE, Schneider O, Vorholt JA, Ellingsen TE, Brautaset T	PLoS One	10.1371/journal.pone.0059188	2013	*
Genetics	Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol.	Heggeset TM, Krog A, Balzer S, Wentzel A, Ellingsen TE, Brautaset T	Appl Environ Microbiol	10.1128/AEM.00703-12	2012	*
Phylogeny	Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough.	Bae SS, Lee JH, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63424-0	2005	*
Cultivation	Growth of Bacillus methanolicus in seawater-based media.	Komives CF, Cheung LY, Pluschkell SB, Flickinger MC	J Ind Microbiol Biotechnol	10.1007/s10295-004-0195-9	2005	*
Phylogeny	Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria.	Arfman N, Dijkhuizen L, Kirchhof G, Ludwig W, Schleifer KH, Bulygina ES, Chumakov KM, Govorukhina NI, Trotsenko YA, White D, et al.	Int J Syst Bacteriol	10.1099/00207713-42-3-439	1992	*

References

#6446

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16454

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70887

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8358.1 )

* These data were automatically processed and therefore are not curated

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